Sprouting of intact motor neurons induced by neuronal lesion in the absence of denervated muscle fibers and degenerating axons

1978 ◽  
Vol 155 (2) ◽  
pp. 354-356 ◽  
Author(s):  
Shlomo Rotshenker
Keyword(s):  
Motor Neurons ◽  
Muscle Fibers ◽  
Denervated Muscle ◽  
Degenerating Axons

scholarly journals Development of a human neuromuscular tissue-on-a-chip model on a 24-well-plate-format compartmentalized microfluidic device

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Kazuki Yamamoto ◽  
Nao Yamaoka ◽  
Yu Imaizumi ◽  
Takunori Nagashima ◽  
Taiki Furutani ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Microfluidic Device ◽  
Motor Neurons ◽  
Muscle Fibers ◽  
Three Dimensional ◽  
Tissue Model ◽  
Skeletal Muscle Fibers

A three-dimensional human neuromuscular tissue model that mimics the physically separated structures of motor neurons and skeletal muscle fibers is presented.

scholarly journals A three-dimensional culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction and disease modeling

2018 ◽  
Author(s):  
Mohsen Afshar Bakooshli ◽  
Ethan S Lippmann ◽  
Ben Mulcahy ◽  
Nisha R Iyer ◽  
Christine T Nguyen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Neuromuscular Junction ◽  
Motor Neuron ◽  
Motor Neurons ◽  
Human Skeletal Muscle ◽  
Disease Modeling ◽  
De Novo ◽  
Muscle Fibers ◽  
Three Dimensional ◽  
Adult Human

SummaryTwo-dimensional (2D) human skeletal muscle fiber cultures are ill equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron endplates. Here we describe a three-dimensional (3D) co-culture method whereby human muscle progenitors mixed with human pluripotent stem cell-derived motor neurons self-organize to form functional NMJ connections within two weeks. Functional connectivity between motor neuron endplates and muscle fibers is confirmed with calcium transient imaging and electrophysiological recordings. Notably, we only observed epsilon acetylcholine receptor subunit protein upregulation and activity in 3D co-culture. This demonstrates that the 3D co-culture system supports a developmental shift from the embryonic to adult form of the receptor that does not occur in 2D co-culture. Further, 3D co-culture treatments with myasthenia gravis patient sera shows the ease of studying human disease with the system. This work delivers a simple, reproducible, and adaptable method to model and evaluate adult human NMJ de novo development and disease in culture.

scholarly journals Denervated muscle fibers induce mitochondrial peroxide generation in neighboring innervated fibers: Role in muscle aging

2017 ◽  
Vol 112 ◽  
pp. 84-92 ◽  
Author(s):  
Natalie Pollock ◽  
Caroline A. Staunton ◽  
Aphrodite Vasilaki ◽  
Anne McArdle ◽  
Malcolm J. Jackson
Keyword(s):  
Muscle Fibers ◽  
Denervated Muscle ◽  
Muscle Aging

scholarly journals Breathing: Motor Control of Diaphragm Muscle

Physiology ◽  
2018 ◽  
Vol 33 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Matthew J. Fogarty ◽  
Carlos B. Mantilla ◽  
Gary C. Sieck
Keyword(s):  
Neural Network ◽  
Motor Control ◽  
Motor Unit ◽  
Motor Neurons ◽  
Muscle Fibers ◽  
Motor Units ◽  
Diaphragm Muscle ◽  
Final Output ◽  
Unit Organization

Breathing occurs without thought but is controlled by a complex neural network with a final output of phrenic motor neurons activating diaphragm muscle fibers (i.e., motor units). This review considers diaphragm motor unit organization and how they are controlled during breathing as well as during expulsive behaviors.

INFLUENCES OF HYPOXIA ON HISTOCHEMICAL PROPERTIES OF THE SOLEUS MUSCLE FIBERS AND MOTOR NEURONS IN RAT

1980 ◽  
Vol 21 (Supplement) ◽  
pp. S62
Author(s):  
S. Taguchi ◽  
K. Itoh ◽  
M. Roh ◽  
Y. Hata ◽  
T. Moritani ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Soleus Muscle ◽  
Muscle Fibers
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