Intrinsic contractile dysfunction in a surgical model of muscle hypertrophy

Synergistic ablation (SA) is widely used to induce muscle hypertrophy in rodent studies. However, it has been demonstrated that SA-induced compensatory hypertrophy induces increases in maximum isometric force that are smaller in magnitude than the increase in muscle cross-sectional area, suggesting a reduction in the specific force production due to intrinsic contractile dysfunction in the hypertrophied fibers. Here, by using the mechanical skinned fibers, we investigated the mechanisms behind the reduction in specific force in the compensatory hypertrophied muscles. Rats had unilateral surgical ablation of the gastrocnemius and soleus muscles to induce the compensatory hypertrophy in the plantaris muscles. Two wk after surgery, the mean fiber diameter was increased by 19% in the SA group compared with the contralateral control (CNT) group. In contrast, compared with the CNT group, both the depolarization-induced force (−51%) and the Ca2+-activated maximum specific force (−32%) were markedly reduced in skinned fibers from the SA group. These deleterious functional alterations were accompanied by decreases in the amount of DHPRα1, RYR, junctophilin 1, and SH3 and cysteine-rich domain 3 (STAC3) in SA muscles. Thus, these data clearly show that SA induces not only an increase in skeletal muscle fiber hypertrophy but also leads to a reduction in the intrinsic contractile dysfunction due to the excitation–contraction uncoupling and impaired force-generating capacity.

Resting Tendon Cross-Sectional Area Underestimates Biceps Brachii Tendon Stress: Importance of Measuring During a Contraction

Force produced by the muscle during contraction is applied to the tendon and distributed through the cross-sectional area (CSA) of the tendon. This ratio of force to the tendon CSA is quantified as the tendon mechanical property of stress. Stress is traditionally calculated using the resting tendon CSA; however, this does not take into account the reductions in the CSA resulting from tendon elongation during the contraction. It is unknown if calculating the tendon stress using instantaneous CSA during a contraction significantly increases the values of in vivo distal biceps brachii (BB) tendon stress in humans compared to stress calculated with the resting CSA. Nine young (22 ± 1 years) and nine old (76 ± 4 years) males, and eight young females (21 ± 1 years) performed submaximal isometric elbow flexion tracking tasks at force levels ranging from 2.5 to 80% maximal voluntary contraction (MVC). The distal BB tendon CSA was recorded on ultrasound at rest and during the submaximal tracking tasks (instantaneous). Tendon stress was calculated as the ratio of tendon force during contraction to CSA using the resting and instantaneous measures of CSA, and statistically evaluated with multi-level modeling (MLM) and Johnson–Neyman regions of significance tests to determine the specific force levels above which the differences between calculation methods and groups became statistically significant. The tendon CSA was greatest at rest and decreased as the force level increased (p < 0.001), and was largest in young males (23.0 ± 2.90 mm2) followed by old males (20.87 ± 2.0 mm2) and young females (17.08 ± 1.54 mm2) (p < 0.001) at rest and across the submaximal force levels. Tendon stress was greater in the instantaneous compared with the resting CSA condition, and young males had the greatest difference in the values of tendon stress between the two conditions (20 ± 4%), followed by old males (19 ± 5%), and young females (17 ± 5%). The specific force at which the difference between the instantaneous and resting CSA stress values became statistically significant was 2.6, 6.6, and 10% MVC for old males, young females, and young males, respectively. The influence of using the instantaneous compared to resting CSA for tendon stress is sex-specific in young adults, and age-specific in the context of males. The instantaneous CSA should be used to provide a more accurate measure of in vivo tendon stress in humans.

Investigation of Lateral Confinement, Roller Aspect Ratio and Wear Condition on HPGR Performance Using DEM-MBD-PRM Simulations

It has been known that the performance of high-pressure grinding rolls (HPGR) varies as a function of the method used to laterally confine the rolls, their diameter/length (aspect) ratio as well as their condition, if new or worn. However, quantifying these effects through direct experimentation in machines with reasonably large dimensions is not straightforward, given the challenge, among others, of guaranteeing that the feed material remains unchanged. The present work couples the discrete element method (DEM) to multibody dynamics (MBD) and a novel particle replacement model (PRM) to simulate the performance of a pilot-scale HPGR grinding pellet feed. It shows that rotating side plates, in particular when fitted with studs, will result in more uniform forces along the bed, which also translates in a more constant product size along the rolls as well as higher throughput. It also shows that the edge effect is not affected by roll length, leading to substantially larger proportional edge regions for high-aspect ratio rolls. On the other hand, the product from the center region of such rolls was found to be finer when pressed at identical specific forces. Finally, rolls were found to have higher throughput, but generate a coarser product when worn following the commonly observed trapezoidal profile. The approach often used in industry to compensate for roller wear is to increase the specific force and roll speed. It has been demonstrated to be effective in maintaining product fineness and throughput, as long as the minimum safety gap is not reached.

Concurrent validity and reliability of a functional electromechanical dynamometer to assess isometric mid-thigh pull performance

The aim of this study was to examine the concurrent validity and reliability of a functional electromechanical dynamometer (FEMD) to assess the isometric mid-thigh pull (IMTP) kinetic variables: peak force (PF), rate of force development (RFD), and time-specific force values (50-, 100-, 150-, and 200-ms). Twenty-seven male collegiate athletes (age: 22.9 ± 1.9 years; stature: 184.8 ± 10.4 cm; body mass: 84.2 ± 11.8 kg) performed four IMTP trials simultaneously on a force platform and the FEMD. The PF variables calculated from performance of the IMTP on both devices were reliable (CV < 3%; ICC > 0.90) and valid (bias < 13.9 N; random error < 52.1 N; r = 1.00), but they showed heteroscedasticity of the errors ( R2 > 0.23). The RFD (CV > 10.88%; ICC < 0.76) and initial force (CV > 8.81%; ICC < 0.76) variables did not reach an acceptable reliability for any device, but they showed strong associations between them ( r range = 0.53–0.69). These results indicate that the FEMD is an acceptable alternative to assess athlete’s maximal force production (i.e. PF), but the data collected with FEMD and force plates should not be used interchangeably.

False cue influence on motion cue quality for 10 motion cueing algorithms

Motion simulators are becoming increasingly popular for many applications in which human sensation is important to replicate and optimize target motions. For the emulation of the perceived human acceleration, motion cueing algorithms (MCAs) have been proposed in the literature that mimics the motion sensation by a combination of actual acceleration and tilted gravity effects, termed g-force or specific force. However, their relative performance has not yet been analyzed. This paper reviews existing families of MCAs and compares their performance for a simple offline S-shaped planar test trajectory featuring only lateral acceleration. The comparison is carried out both numerically using two previously published objective measures, the “performance indicator” of Pouliot, Gosselin, and Nahon, and the “good criterion” of Schmidt, as well as subjectively by a preliminary passenger rating on a real motion platform—Robocoaster testbed. The results show that (a) the novel optimizing MCA group exploits more effectively the workspace of the motion platform than the traditional MCA group for reducing false cue with small scale error and shape errors, (b) path-dependent tuning of MCA parameters may improve motion sensation, (c) average subjective ratings can be made to correlate well with the “good criterion” when expanded with a penalty for false angular velocity cues, and (d) the scale error of specific force seems to play the most important role to the evaluation of test subject on the motion cue quality. However, still a strong variance in subjective ratings was observed, making further research necessary.

Contractile Properties of MHC I and II Fibers From Highly Trained Arm and Leg Muscles of Cross-Country Skiers

IntroductionLittle is known about potential differences in contractile properties of muscle fibers of the same type in arms and legs. Accordingly, the present study was designed to compare the force-generating capacity and Ca2+ sensitivity of fibers from arm and leg muscles of highly trained cross-country skiers.MethodSingle muscle fibers of m. vastus lateralis and m. triceps brachii of eight highly trained cross-country skiers were analyzed with respect to maximal Ca2+-activated force, specific force and Ca2+ sensitivity.ResultThe maximal Ca2+-activated force was greater for myosin heavy chain (MHC) II than MHC I fibers in both the arm (+62%, P &lt; 0.001) and leg muscle (+77%, P &lt; 0.001), with no differences between limbs for each MHC isoform. In addition, the specific force of MHC II fibers was higher than that of MHC I fibers in both arms (+41%, P = 0.002) and legs (+95%, P &lt; 0.001). The specific force of MHC II fibers was the same in both limbs, whereas MHC I fibers from the m. triceps brachii were, on average, 39% stronger than fibers of the same type from the m. vastus lateralis (P = 0.003). pCa50 was not different between MHC I and II fibers in neither arms nor legs, but the MHC I fibers of m. triceps brachii demonstrated higher Ca2+ sensitivity than fibers of the same type from m. vastus lateralis (P = 0.007).ConclusionComparison of muscles in limbs equally well trained revealed that MHC I fibers in the arm muscle exhibited a higher specific force-generating capacity and greater Ca2+ sensitivity than the same type of fiber in the leg, with no such difference in the case of MHC II fibers. These distinct differences in the properties of fibers of the same type in equally well-trained muscles open new perspectives in muscle physiology.