Effect of n-hexane inhalation on the monooxygenase system in MICE liver microsomes

1974 ◽  
Vol 8 (1) ◽  
pp. 11-18 ◽  
Author(s):  
A. Krämer ◽  
Hj. Staudinger ◽  
V. Ullrich
Keyword(s):  
Liver Microsomes ◽  
Monooxygenase System ◽  
Mice Liver

Mice liver microsomes stabilize erythrocytes with respect to lysis induced by trifluoroperazine

1996 ◽  
Vol 30 (11) ◽  
pp. 671-674
Author(s):  
N. K. Meirelles ◽  
S. V. P. Maleiros ◽  
A. S. Rugeiro ◽  
N. K. Tatarskaya ◽  
G. E. Zaikov ◽  
...  
Keyword(s):  
Liver Microsomes ◽  
Mice Liver

Monooxygenase System and NO Metabolism in Liver Microsomes of Rats with Toxic Hepatitis and the Effect of Sesquiterpene Lactones

2021 ◽  
Vol 172 (2) ◽  
pp. 133-136
Author(s):  
N. V. Tursunova ◽  
V. N. Syrov ◽  
Z. A. Khushbaktova ◽  
Yu. V. Tornuev ◽  
M. G. Klinnikova
Keyword(s):  
Toxic Hepatitis ◽  
Sesquiterpene Lactones ◽  
Liver Microsomes ◽  
Monooxygenase System

A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P450-Mediated O-Demethylation Using a Novel Coumarin Analog as Substrate

2000 ◽  
Vol 55 (11-12) ◽  
pp. 915-922 ◽  
Author(s):  
René Roser ◽  
Helmut Thomas
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Fluorometric Assay ◽  
Excitation Wavelength ◽  
Monooxygenase System ◽  
Rat Liver Microsomes ◽  
Monooxygenase Activity ◽  
Continuous Increase ◽  
Highly Sensitive

A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (λA = 380 nm, λF = 480 nm ) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl2and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 480 nm and a fluorescence/em ission w avelength of 480 nm, the fluorescence of this substance (λA = 338 nm, λF = 422 nm ) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrom e P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.

The presence of a monooxygenase system in human fetal liver microsomes

Life Sciences ◽  
1970 ◽  
Vol 9 (20) ◽  
pp. 1189-1200 ◽  
Author(s):  
S.J. Yaffe ◽  
A. Rane ◽  
F. Sjöqvist ◽  
L.-O. Boréus ◽  
S. Orrenius
Keyword(s):  
Fetal Liver ◽  
Liver Microsomes ◽  
Monooxygenase System ◽  
Human Fetal Liver

Sex difference in responsiveness to aztreonam of monooxygenase system in liver microsomes from rats

1987 ◽  
Vol 36 (7) ◽  
pp. 1053-1057 ◽  
Author(s):  
Tohru Horie ◽  
Mitsukazu Kitada ◽  
Yoshio Tanabe ◽  
Yoshio Kanakubo
Keyword(s):  
Sex Difference ◽  
Liver Microsomes ◽  
Monooxygenase System
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