Electrical activity of rat brain as a correlate of primary drive

1962 ◽  
Vol 14 (2) ◽  
pp. 233-243 ◽  
Author(s):  
William G. Steiner
Keyword(s):  
Electrical Activity ◽  
Rat Brain ◽  
Primary Drive

scholarly journals CRYOPRESERVATION OF RAT BRAIN SLICES WITH DIFFERENT DURATION AND RESTORATION OF THEIR ELECTRICAL ACTIVITY

Biomeditsina ◽  
2019 ◽  
pp. 98-106
Author(s):  
A. A. Mokrushin
Keyword(s):  
Action Potential ◽  
Electrical Activity ◽  
Rat Brain ◽  
Brain Slices ◽  
Wistar Rat ◽  
Lateral Olfactory Tract ◽  
Olfactory Tract

Effects of cryopreservation of male Wistar rat brain slices with different duration (4, 8, 10, 23, and 90 days) on changes in their electrical activity was investigated. The amplitudes of AMPA- and NMDA-dependent glutamatergic ionotropic mechanisms were measured, as well as the action potential of the lateral olfactory tract (AP LOT) at a temperature of –20°C and subsequent warming to +37°C. After cryopreservation, these mechanisms were preserved and restored. We used the method of electrophysiological registration of the AMPA and NMDA potentials and the total AP LOT. After cryopreservation, the AMPA-dependent mechanisms and the activity of conductive LOT fi bers were restored to normothermal values. On the contrary, the recovery of NMDA-dependent mechanisms was incomplete and averaged 34% compared with the normothermal values. The results indicate that, after cryopreservation, the activity of basic ionotropic glutamatergic mechanisms in rat brain slices is restored.

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