Cell surface charge, sialic acid content and metabolic behaviour of two tumour sublines. A comparative study

1977 ◽  
Vol 13 (10) ◽  
pp. 1145-1150 ◽  
Author(s):  
B. Bohn ◽  
C. Thies ◽  
R. Brossmer
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Comparative Study ◽  
Surface Charge ◽  
Acid Content ◽  
Sialic Acid Content ◽  
Cell Surface Charge ◽  
Metabolic Behaviour

Human X- and Y-bearing sperm differ in cell surface sialic acid content

1984 ◽  
Vol 124 (3) ◽  
pp. 950-955 ◽  
Author(s):  
Satoru Kaneko ◽  
Shigeru Oshio ◽  
Toshifumi Kobayashi ◽  
Rihachi Iizuka ◽  
Hideo Mohri
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Acid Content ◽  
Sialic Acid Content

scholarly journals Sialic acid content and surface hydrophobicity of group B streptococci

1993 ◽  
Vol 110 (1) ◽  
pp. 87-94 ◽  
Author(s):  
L. A. Teixeira ◽  
A. M. S. Figueiredo ◽  
B. T. Ferreira ◽  
V. M. L. Alves ◽  
P. E. Nagao ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Acid Content ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Gas Liquid Chromatography ◽  
Group B Streptococci ◽  
Sialic Acid Content ◽  
Asymptomatic Patients ◽  
Group B

SUMMARYThe sialic acid content and the cell-surface hydrophobicity index of 40 group B streptococci (GBS) strains were assessed. GBS isolated from invasive infections (virulent strains) presented an increased level of sialic acid content (1.4%) when compared with GBS isolated from asymptomatic patients (0.53%). Treatment of GBS strain 85634 with neuraminidase resulted in a decrease (about 25%) in the net negative surface charge as assessed by cell electrophoresis. This finding suggests that sialic acid residues are important anionogenic groups exposed on GBS cell surface.N-acetylneuraminic acid was the only sialic acid derivative characterized in the strain 85634 as evaluated by gas-liquid chromatography. GBS from different serotypes presented a hydrophobic index mean value of 0.9. Even though the sialic acid contributed effectively to the negative charge on GBS cell surface, no difference was observed in the hydrophobic index when virulent and avirulent strains were compared.

Role of sialic acid in insulin action and the insulin resistance of diabetes mellitus

1988 ◽  
Vol 255 (2) ◽  
pp. E173-E179 ◽  
Author(s):  
A. I. Salhanick ◽  
J. M. Amatruda
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Sialic Acid ◽  
Cell Surface ◽  
Acid Content ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Hepatic Insulin Resistance ◽  
Sialic Acid Content ◽  
Insulin Responsiveness

Adipocytes treated with neuraminidase show markedly reduced responsiveness to insulin without any alteration in insulin binding. In addition, several studies have separately demonstrated both insulin resistance and decreases in membrane sialic acid content and associated biosynthetic enzymes in diabetes mellitus. In the present study, we investigated the role that sialic acid residues may play in insulin action and in the hepatic insulin resistance associated with nonketotic diabetes. Primary cultures of hepatocytes from normal rats treated with neuraminidase demonstrated a dose-dependent decrease in insulin-stimulated lipogenesis. At a concentration of neuraminidase that decreases insulin action by 50%, 23% of total cellular sialic acid content was released. Neuraminidase-releasable sialic acid was significantly decreased in hepatocytes from diabetic rats and this was associated with significant insulin resistance. Treatment of hepatocytes from diabetic rats with cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NANA) enhanced insulin responsiveness 39%. The enhanced insulin responsiveness induced by CMP-NANA was blocked by cytidine 5'-monophosphate (CMP) suggesting that the CMP-NANA effect was catalyzed by a cell surface sialyltransferase. CMP reduced neuraminidase-releasable [14C]sialic acid incorporation into hepatocytes by 43%. The data demonstrate a role for cell surface sialic acid residues in hepatic insulin action and support a role for decreased cell surface sialic acid residues in the insulin resistance of diabetes mellitus.

Target of X Irradiation and Dislocation of Sialic Acid in Decrease of Cell Surface Charge of Erythrocytes

1977 ◽  
Vol 69 (2) ◽  
pp. 367 ◽  
Author(s):  
Chikako Sato ◽  
Kiyohide Kojima ◽  
Kimiko Nishizawa
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Charge ◽  
X Irradiation

Erythrocyte membrane bound and plasma sialic acid during aging

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Dileep Kumar ◽  
Syed Rizvi
Keyword(s):  
Sialic Acid ◽  
Surface Charge ◽  
Erythrocyte Membrane ◽  
Acid Content ◽  
Acute Phase Proteins ◽  
Sialic Acid Content ◽  
Negative Surface ◽  
Negative Surface Charge ◽  
Membrane Bound ◽  
Bulk Charge

AbstractSialic acid, a nine-carbon sugar, is an acetylated derivative of neuraminic acid predominantly found in vertebrates, a few higher invertebrates, and certain types of bacteria. Red blood cells (RBCs) have a net negative surface charge and this bulk charge is due to ionized sialic acid. Decreased surface charge and sialic acid content have been reported in older erythrocytes, and it is postulated that the decreased electro-negativity may be related to cell senescence. In the present study we report the RBC and plasma sialic acid content during aging in rats. Our results show a significant decrease in RBC sialic acid content and increase in plasma sialic acid as a function of rat aging. The decreased sialic acid in erythrocyte membrane with increasing rat age presents a good biomarker of the aging process. The elevated plasma sialic acid may be a manifestation of several factors including increased expression of acute phase proteins and increased damage to various organs.

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