Nuclear protein synthesis at permissive and non-permissive temperatures in a Chinese hamster ovary cell line with a temperature-sensitive defect in cytoplasmic non-mitochondrial protein synthesis

1979 ◽  
Vol 121 (1) ◽  
pp. 191-207 ◽  
Author(s):  
K.M. Anderson ◽  
B. Bass ◽  
M. Rubenstein ◽  
T. Saclarides
Keyword(s):  
Protein Synthesis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Nuclear Protein ◽  
Mitochondrial Protein ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Temperature Sensitive ◽  
Mitochondrial Protein Synthesis ◽  
Ovary Cell Line

Discrete electrophoretic products of mitochondrial protein synthesis in the Chinese hamster ovary cell line

1977 ◽  
Vol 55 (10) ◽  
pp. 1064-1074 ◽  
Author(s):  
R. W. Yatscoff ◽  
K. B. Freeman
Keyword(s):  
Protein Synthesis ◽  
Cell Line ◽  
Electrophoretic Mobility ◽  
Chinese Hamster Ovary ◽  
Mitochondrial Protein ◽  
Chinese Hamster ◽  
Mitochondrial Proteins ◽  
Mitochondrial Protein Synthesis ◽  
Cho Cell ◽  
Cho Cell Line

Mitochondrial proteins labelled with [35S]methionine for 1 h in whole Chinese hamster ovary (CHO) cells in the presence of cycloheximide or emetine, known inhibitors of cytosolic protein synthesis, have been enumerated and characterized by their electrophoretic mobility in sodium dodecyl sulfate slab gel electrophoresis. Ten distinct electrophoretic bands were observed. The components were relatively stable during a 2 h postlabelling period. The same 10 bands were also seen with the CHO cell line tsH1, labelled at 40 °C, a temperature at which cytosolic but not mitochondrial protein synthesis is inhibited in this cell line, and with isolated mitochondria labelled in the presence of cycloheximide. An 11th band was present when [3H]leucine but not [35S] methionine was used for labelling. The width of the major band suggested that it consists of two components making a total of at least 12 proteins synthesized in mitochondria. The molecular weights of these mitochondrial proteins ranged from 5000 to 50000 and there was a sixfold difference in the relative molar amounts synthesized in a 1-h period in the presence of [3H]leucine or [3SS] methionine.No differences in number or electrophoretic mobility of the mitochondrially synthesized proteins were found among the seven CHO cell lines examined. These results suggest the stability of the mitochondrial genome in the CHO cell line.

Lipid Requirements of a Recombinant Chinese Hamster Ovary Cell Line (CHO)

2006 ◽  
pp. 205-207 ◽  
Author(s):  
Jeffrey T. Mcgrew ◽  
Cheryl L. Richards ◽  
Pauline Smidt ◽  
Bradley Dell ◽  
Virginia Price
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Ovary Cell Line
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