scholarly journals Effect of H1 protein on in vitro ribosomal RNA synthesis

FEBS Letters ◽  
1974 ◽  
Vol 43 (1) ◽  
pp. 86-88 ◽  
Author(s):  
Andrew Travers ◽  
Regine Cukier-Kahn
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis

scholarly journals DIFFERENTIATION OF DROSOPHILA CELLS LACKING RIBOSOMAL DNA, IN VITRO

Genetics ◽  
1973 ◽  
Vol 73 (3) ◽  
pp. 429-434
Author(s):  
J James Donady ◽  
R L Seecof ◽  
M A Fox
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribosomal Dna ◽  
Rna Synthesis ◽  
Wild Type ◽  
Drosophila Cells

ABSTRACT Drosophila melanogaster embryos that lacked ribosomal DNA were obtained from appropriate crosses. Cells were taken from such embryos before overt differentiation took place and were cultured in vitro. These cells differentiated into neurons and myocytes with the same success as did wild-type controls. Therefore, ribosomal RNA synthesis is not necessary for the differentiation of neurons and myocytes in vitro.

Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro

Development ◽  
1974 ◽  
Vol 32 (2) ◽  
pp. 515-532
Author(s):  
A. Colman
Keyword(s):  
Xenopus Laevis ◽  
In Vitro Culture ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Salt Medium ◽  
Complex Media ◽  
18S Ribosomal Rna ◽  
Rna Precursor

RNA synthesis can be maintained in large oocytes of Xenopus laevis during periods of in vitro culture of at least 10 days. A simple salt medium, modified Barth's solution, is found to be as effective a culture medium for these oocytes as several other complex media. The newly synthesized RNA is characterized electrophoretically and shown to consist predominantly of ribosomal RNA precursor, 28S and 18S ribosomal RNA, and 4S RNA. The distribution of this RNA within the oocyte is detected autoradiographically, where it is found to be greatly concentrated over the nucleoli. No qualitative alterations in either of these parameters are found during culture, within the limits of sensitivity of the assay procedures.

Ribonucleic acid synthesis inPlasmodium knowlesimaintained bothin vivoandin vitro

Parasitology ◽  
1975 ◽  
Vol 71 (2) ◽  
pp. 199-209 ◽  
Author(s):  
P. I. Trigg ◽  
P. G. Shakespeare ◽  
Susan J. Burt ◽  
Sally I. Kyd
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Nuclear Division ◽  
Plasmodium Knowlesi ◽  
Acid Synthesis ◽  
Agarose Gel Electrophoresis ◽  
Trophozoite Stage ◽  
Late Trophozoite

RNA extracted from purified parasites ofPlasmodium knowlesiwas fractionated using agarose gel electrophoresis. Preparations from parasites grown bothin vivoandin vitrocontained species of RNA with sedimentation coefficients of 4·0S, 5·0S, 16·6S, 24·2S, 31·4S, 38·0S and 48·3S. There was less RNA present in parasites grownin vitrothan the equivalent stage parasites grownin vivobut the proportional amounts of the various species of RNA was similar in both cases. It is suggested that the 24·2S and 16·6S species of RNA are ribosomal and that the high molecular weight 31·4S, 38·0S and 48·0S species are ribosomal precursors. Ribosomal RNA synthesis occurs throughout the cell cycle during growth from the ring to the schizont stage; maximum incorporation of [H3]-adenosine occurs at the late trophozoite stage before nuclear division.

scholarly journals Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis

2019 ◽  
Vol 47 (12) ◽  
pp. 6488-6503 ◽  
Author(s):  
Yong-Heng Huang ◽  
Nelly Said ◽  
Bernhard Loll ◽  
Markus C Wahl
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Rna Folding ◽  
In Vitro Transcription ◽  
Structural Basis ◽  
Macromolecular Crystallography ◽  
Rna Transcription ◽  
Signal Element

AbstractRibosomal RNA synthesis in Escherichia coli involves a transcription complex, in which RNA polymerase is modified by a signal element on the transcript, Nus factors A, B, E and G, ribosomal protein S4 and inositol mono-phosphatase SuhB. This complex is resistant to ρ-dependent termination and facilitates ribosomal RNA folding, maturation and subunit assembly. The functional contributions of SuhB and their structural bases are presently unclear. We show that SuhB directly binds the RNA signal element and the C-terminal AR2 domain of NusA, and we delineate the atomic basis of the latter interaction by macromolecular crystallography. SuhB recruitment to a ribosomal RNA transcription complex depends on the RNA signal element but not on the NusA AR2 domain. SuhB in turn is required for stable integration of the NusB/E dimer into the complex. In vitro transcription assays revealed that SuhB is crucial for delaying or suppressing ρ-dependent termination, that SuhB also can reduce intrinsic termination, and that SuhB-AR2 contacts contribute to these effects. Together, our results reveal functions of SuhB during ribosomal RNA synthesis and delineate some of the underlying molecular interactions.

scholarly journals Mobility restriction in vivo of the heavy ribosomal subunit in a secretory cell.

1976 ◽  
Vol 70 (3) ◽  
pp. 573-580 ◽  
Author(s):  
U Lönn ◽  
J E Edström
Keyword(s):  
Endoplasmic Reticulum ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Ribosomal Subunit ◽  
Chironomus Tentans ◽  
Salivary Gland Cells ◽  
Unique Parameter ◽  
Labeled Rna

Analysis in insect (Chironomus tentans) salivary gland cells of labeled RNA as a function of time after precursor injection and its distance to the nuclear membrane, cytoplasmic zone analysis, could previously be used to demonstrate the presence of short-lasting gradients in newly labeled ribosomal RNA. Since the gradients were sensitive to puromycin, they are likely to be a result of diffusion restriction due to an engagement of the subunits into polysomes. In the present paper the possibility was explored of recording gradients that were caused by labeled subunits in puromycin-resistant associations, which, in all probability, involve the endoplasmic reticulum. It was found that labeled 28 S and 5 S RNA but not 18 S RNA were present in radioactivity gradients lasting for at least 2 days but less than 6 days. The gradients also remained during inhibition of RNA synthesis by actinomycin, and they were completely resistant to puromycin whether given in vivo or in vitro. The semipermanent gradients formed fhere offer a unique parameter for the in vivo study of conditions for formation and maintenance of heavy subunits in puromycin-resistant bonds. An explanation for these and previous results is that the light subunit, although restricted in movement by engagement to polysomes, is nevertheless free to exchange and spread between rounds of translation, whereas at least part of the heavy subunit population is bound to the endoplasmic reticulum and less free to spread. These results offer a good in vivo correlate to previous results on in vitro exchangeability of subunits.

scholarly journals In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

2013 ◽  
Vol 9 (1) ◽  
pp. 678 ◽  
Author(s):  
Michael C Jewett ◽  
Brian R Fritz ◽  
Laura E Timmerman ◽  
George M Church
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Ribosome Assembly

RNA SYNTHESIS IN THE RAT ANTERIOR HYPOTHALAMUS AND PITUITARY: RELATION TO NEONATAL ANDROGEN AND THE OESTROUS CYCLE

1970 ◽  
Vol 48 (1) ◽  
pp. 125-137 ◽  
Author(s):  
D. F. SALAMAN
Keyword(s):  
Ribosomal Rna ◽  
Anterior Pituitary ◽  
Rna Synthesis ◽  
Specific Activity ◽  
Gradient Centrifugation ◽  
Anterior Hypothalamus ◽  
Oestrous Cycle ◽  
Uridine Incorporation ◽  
Cyclic Changes

SUMMARY RNA from the anterior hypothalamus and anterior pituitary of rats has been labelled by incubation in vitro with [3H]uridine and characterized by density gradient centrifugation. A study of normal females during the oestrous cycle showed cyclic changes in [3H]uridine incorporation into rapidly labelled RNA (rl-RNA) both in the anterior pituitary and hypothalamus. In both tissues the specific activity of RNA was low at dioestrus and high at oestrus and metoestrus. In androgenized females, incorporation into hypothalamic rl-RNA was less than the oestrus—metoestrus level and similar to that at dioestrus, while incorporation into anterior pituitary rl-RNA was similar to the oestrus—metoestrus level and greater than at dioestrus. [3H]Uridine incorporation into ribosomal RNA (r-RNA) of anterior hypothalamus and pituitary was also demonstrated by incubation for 4 h. Under these conditions there was no effect of androgenization on hypothalamic r-RNA, but the specific activity of pituitary r-RNA was greater than normal.

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