Efficient production of native, biologically active human cystatin C by Escherichia coli

FEBS Letters ◽  
1988 ◽  
Vol 236 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Magnus Abrahamson ◽  
Henrik Dalbøge ◽  
Isleifur Olafsson ◽  
Søren Carlsen ◽  
Anders Grubb
Keyword(s):  
Escherichia Coli ◽  
Cystatin C ◽  
Biologically Active ◽  
Efficient Production ◽  
Human Cystatin C ◽  
Human Cystatin

scholarly journals piggyBac Transposition and the Expression of Human Cystatin C in Transgenic Chickens

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1554
Author(s):  
Seo Woo Kim ◽  
Jeong Hyo Lee ◽  
Ji Seon Han ◽  
Seung Pyo Shin ◽  
Tae Sub Park
Keyword(s):  
Cystatin C ◽  
Mass Production ◽  
Animal Cell ◽  
Egg White ◽  
Efficient Production ◽  
Animal Cells ◽  
Bioactive Substances ◽  
Human Cystatin C ◽  
Transgenic Chickens ◽  
Human Cystatin

A bioreactor can be used for mass production of therapeutic proteins and other bioactive substances. Although various methods have been developed using microorganisms and animal cells, advanced strategies are needed for the efficient production of biofunctional proteins. In microorganisms, post-translational glycosylation and modification are not performed properly, while animal cell systems require more time and expense. To overcome these problems, new methods using products from transgenic animals have been considered, such as genetically modified cow’s milk and hen’s eggs. In this study, based on a non-viral piggyBac transposition system, we generated transgenic bioreactor chickens that produced human cystatin C (hCST3). There were no differences in the phenotype or histochemical structure of the wild-type and hCST3-expressing transgenic chickens. Subsequently, we analyzed the hCST3 expression in transgenic chickens, mainly in muscle and egg white, which could be major deposition warehouses for hCST3 protein. In both muscle and egg white, we detected high hCST3 expression by ELISA and Western blotting. hCST3 proteins were efficiently purified from muscle and egg white of transgenic chickens using a His-tag purification system. These data show that transgenic chickens can be efficiently used as a bioreactor for the mass production of bioactive materials.

High-level expression of active human cystatin C in Escherichia coli

Gene ◽  
1989 ◽  
Vol 79 (2) ◽  
pp. 325-332 ◽  
Author(s):  
H. Dalbøge ◽  
E.Bech Jensen ◽  
H. Tøttrup ◽  
A. Grubb ◽  
M. Abraharmson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cystatin C ◽  
High Level Expression ◽  
Human Cystatin C ◽  
High Level ◽  
Human Cystatin

Affinity Purification and Elimination of Methionine Oxidation in Recombinant Human Cystatin C

1997 ◽  
Vol 11 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Paul J. Berti ◽  
Irena Ekiel ◽  
Peter Lindahl ◽  
Magnus Abrahamson ◽  
Andrew C. Storer
Keyword(s):  
Cystatin C ◽  
Affinity Purification ◽  
Methionine Oxidation ◽  
Human Cystatin C ◽  
Human Cystatin

scholarly journals Application of amide hydrogen/deuterium exchange mass spectrometry for epitope mapping in human cystatin C

Amino Acids ◽  
2016 ◽  
Vol 48 (12) ◽  
pp. 2809-2820 ◽  
Author(s):  
Martyna Prądzińska ◽  
Izabela Behrendt ◽  
Juan Astorga-Wells ◽  
Aleksandr Manoilov ◽  
Roman A. Zubarev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cystatin C ◽  
Epitope Mapping ◽  
Hydrogen Deuterium Exchange ◽  
Amide Hydrogen ◽  
Exchange Mass Spectrometry ◽  
Human Cystatin C ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Human Cystatin
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