An improved method for isolation of Heliothis zea DNA-dependent RNA polymerase: Separation and characterization of a form III RNA polymerase activity

1981 ◽  
Vol 11 (2) ◽  
pp. 149-154 ◽  
Author(s):  
M.A. Grula ◽  
Robert F. Weaver
Keyword(s):  
Rna Polymerase ◽  
Heliothis Zea ◽  
Polymerase Activity ◽  
Improved Method ◽  
Rna Polymerase Activity

scholarly journals COMPARISON AND CHARACTERIZATION OF NUCLEAR ISOLATION PROCEDURES AS APPLIED TO CHICK OVIDUCT

1973 ◽  
Vol 59 (3) ◽  
pp. 685-695 ◽  
Author(s):  
John T. Knowler ◽  
Harold L. Moses ◽  
Thomas C. Spelsberg
Keyword(s):  
Citric Acid ◽  
Rna Polymerase ◽  
Polymerase Activity ◽  
Isolation Procedure ◽  
High Yield ◽  
Rna Integrity ◽  
Nuclear Rna ◽  
Rna Polymerase Activity ◽  
Nuclear Isolation

A number of methods for the preparation of chick oviduct nuclei have been compared. Nuclei have been isolated in hypertonic sucrose and citric acid and the product has been characterized with respect to cleanliness, ultrastructure, RNA polymerase activity, RNA integrity, and chromatin composition. The study demonstrates that the choice of oviduct nuclear isolation procedure will depend markedly on the purpose for which the nuclei are required. Thus, nuclei prepared entirely in high-molarity sucrose retain the highest levels of RNA polymerase. Those prepared rapidly in the presence of citric acid retain nuclear RNA in an essentially undegraded state. Finally, a bulk preparation is described which, because of its adaptability and high yield of morphologically intact nuclei using large amounts of tissue, is ideal for use in preparing chromatin. Conditions are described by which isolated nuclei can be stored for up to 6 months and retain their morphology, chemical characteristics, and RNA polymerase activity.

Isolation and characterization of macronuclei of Paramecium caudatum infected with the macronucleus-specific bacterium Holospora obtusa

1985 ◽  
Vol 73 (1) ◽  
pp. 389-398
Author(s):  
M. Freiburg
Keyword(s):  
Rna Polymerase ◽  
Polymerase Activity ◽  
Proteinase Activity ◽  
Paramecium Caudatum ◽  
Isolation And Characterization ◽  
Rna Polymerase Activity

Macronuclei from Paramecium caudatum infected with Holospora obtusa may be isolated on sucrose step gradients. Macronuclei containing primarily infectious forms can be separated from those bearing predominantly reproductive forms. RNA polymerase activity in infected macronuclei is greater by a factor of 5 than that in uninfected macronuclei. Proteinase activity is also significantly higher.

Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding

Hepatology ◽  
1999 ◽  
Vol 29 (4) ◽  
pp. 1227-1235 ◽  
Author(s):  
Koji Ishii ◽  
Yoshinobu Tanaka ◽  
Chan-Choo Yap ◽  
Hideki Aizaki ◽  
Yoshiharu Matsuura ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Rna Binding ◽  
Polymerase Activity ◽  
Protein Characterization ◽  
Rna Polymerase Activity ◽  
Ns5b Protein
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