Characterization of free and tightly bound lipoprotein in intima by thin layer isoelectric focusing

1979 ◽  
Vol 33 (3) ◽  
pp. 329-342 ◽  
Author(s):  
Elspeth B. Smith ◽  
Heather S. Dietz ◽  
Isobel B. Craig
Keyword(s):  
Thin Layer ◽  
Isoelectric Focusing

scholarly journals Comparison of polyacrylamide and agarose gel thin-layer isoelectric focusing for the characterization of beta-lactamases.

1983 ◽  
Vol 24 (2) ◽  
pp. 186-189 ◽  
Author(s):  
C Vecoli ◽  
F E Prevost ◽  
J J Ververis ◽  
A A Medeiros ◽  
G P O'Leary
Keyword(s):  
Thin Layer ◽  
Isoelectric Focusing ◽  
Agarose Gel ◽  
Beta Lactamases

Thin-layer chromatographic characterization of metabolites formed from an AMPA antagonist drug candidate

2001 ◽  
Vol 14 (4) ◽  
pp. 266-271 ◽  
Author(s):  
Katalin Migléczi ◽  
István Hazai ◽  
Katalin Jemnitz ◽  
Márta Pátfalusi
Keyword(s):  
Thin Layer ◽  
Drug Candidate ◽  
Ampa Antagonist ◽  
Chromatographic Characterization

scholarly journals Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

1990 ◽  
Vol 111 (4) ◽  
pp. 1639-1643 ◽  
Author(s):  
S C Ho ◽  
M Schindler ◽  
J L Wang
Keyword(s):  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Affinity Column ◽  
Carbohydrate Binding ◽  
Binding Affinities ◽  
Isolation And Characterization ◽  
Relative Binding ◽  
Mannose Derivatives ◽  
Derivatives Of

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.

scholarly journals Synthesis and Characterization of Hydrophobic Silica Thin Layer Derived from Methyltrimethoxysilane (MTMS)

2018 ◽  
Vol 299 ◽  
pp. 012041 ◽  
Author(s):  
Adi Darmawan ◽  
Riyadini Utari ◽  
Riza Eka Saputra ◽  
Suhartana ◽  
Yayuk Astuti
Keyword(s):  
Thin Layer ◽  
Synthesis And Characterization ◽  
Hydrophobic Silica
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