Characterization of regenerated silk fibroin membrane for immobilizing peroxidase and construction of an amperometric hydrogen peroxide sensor employing phenazine methosulphate as electron shuttle

1995 ◽  
Vol 397 (1-2) ◽  
pp. 157-162 ◽  
Author(s):  
Jianghong Qian ◽  
Yongcheng Liu ◽  
Haiying Liu ◽  
Tongyin Yu ◽  
Jiaqi Deng
Keyword(s):  
Hydrogen Peroxide ◽  
Silk Fibroin ◽  
Electron Shuttle ◽  
Hydrogen Peroxide Sensor ◽  
Regenerated Silk Fibroin ◽  
Phenazine Methosulphate

Synthesis and Characterization of a New Cu (BHB)2/Fe-MIL-101-NH2 Composite: A Novel Hydrogen Peroxide Sensor based upon the Bimetallic Complex

2021 ◽  
Vol 168 (1) ◽  
pp. 017508
Author(s):  
Kazem Karami ◽  
Parvaneh Bayat ◽  
Hossein Khosropour ◽  
Firouzeh Siadatnasab ◽  
Behzad Rezaei ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Synthesis And Characterization ◽  
Bimetallic Complex ◽  
Hydrogen Peroxide Sensor

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

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