Optimization of conditions for in vitro antigenic stimulation of dissociated mouse spleen cells for the production of monoclonal antibodies against peptide hormones

1985 ◽  
Vol 78 (2) ◽  
pp. 227-237 ◽  
Author(s):  
D.A. Rathjen ◽  
P.A. Underwood
Keyword(s):  
Monoclonal Antibodies ◽  
Peptide Hormones ◽  
Antigenic Stimulation ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Stimulation Of

scholarly journals In Vitro Immunization of Mouse Spleen Cells and the Production of Monoclonal Antibodies.

1983 ◽  
Vol 37b ◽  
pp. 647-648 ◽  
Author(s):  
Carl A. K. Borrebaeck ◽  
Liselotte Nielsen ◽  
William H. Pirkle ◽  
Ulla Björkroth ◽  
Lu Yi-An ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
In Vitro Immunization

scholarly journals Specific elimination of cytotoxic effector cells. I. Adsorptive behavior of effectors and their precursors and spleen cell monolayers.

1976 ◽  
Vol 144 (4) ◽  
pp. 996-1008 ◽  
Author(s):  
J R Neefe ◽  
D H Sachs
Keyword(s):  
Mouse Spleen ◽  
Spleen Cells ◽  
Effector Cells ◽  
Cell Monolayers ◽  
Specific Suppression ◽  
Normal Spleen ◽  
Adsorptive Behavior ◽  
Cytotoxic Effector ◽  
Primed Mouse

Monolayers formed of normal mouse spleen cells attached to polystyrene coated with poly-L-lysine were tested for their ability to bind specifically antigen-reactive cells in normal or primed mouse spleen. 88 to greater than 98% of the activity of cytotoxic populations was removed by a single adsorption. However, normal spleen cells or spleen cells previously primed in vitro could not be depleted of their capacity to be sensitized, even when adsorption effectively removed all residual cytotoxic activity from the same previously primed population. In fact, exposure to an immunoadsorbent augmented the ultimate cytotoxicity generated in a nonspecific fashion. This augmentation was especially dramatic in the case of a previously primed population and may have reflected the removal of a nonspecific suppressor. If antigen-reactive precursors cannot be removed efficiently by adsorption, other approaches to the generation of tolerant lymphoid populations, such as specific suppression of precursor differentiation must be sought.

Monoclonal antibodies against surface antigens of schistosomula of Schistosoma mansoni

Parasitology ◽  
1982 ◽  
Vol 84 (1) ◽  
pp. 65-82 ◽  
Author(s):  
D. W. Taylor ◽  
A. F. Butterworth
Keyword(s):  
Monoclonal Antibodies ◽  
Gel Electrophoresis ◽  
Primary Infection ◽  
Polyacrylamide Gel Electrophoresis ◽  
Apparent Molecular Weight ◽  
Surface Antigens ◽  
Soft Agar ◽  
Surface Proteins ◽  
Spleen Cells

SUMMARYMonoclonal antibodies have been produced after fusion of NS-1 murine myeloma cells with spleen cells from mice immunized either by chronic primary infection or with irradiated cercariae: in both cases, animals were challenged with live cercariae 7 days before fusion. The initial cultures were screened for anti-schistosomular antibodies both by a radioimmunoassay with whole schistosomulum extracts and by immunofluorescence. There was no correlation between the two techniques and subsequent screening was carried out by immunofluorescence. Cloning was carried out in soft agar and 7 cloned cell lines, from 5 initial cultures, were selected for detailed study. Products of 6 of these 7 lines were monoclonal, as judged by isoelectricfocusing of [35S]methionine-labelled supernatant fluids, and their binding to live schistosomula was specific. None of the antibodies showed detectable activity in mediating eosinophil- or complement-dependent damage to schistosomula in vitro. However, 2 antibodies were successfully used to isolate surface proteins with an apparent molecular weight of 24000 on SDS-polyacrylamide gel electrophoresis.

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