A solid-phase enzyme immunoassay for the common and subtypic determinants of hepatitis B surface antigen with monoclonal antibodies

1986 ◽  
Vol 87 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Sadakazu Usuda ◽  
Fumio Tsuda ◽  
Tohru Gotanda ◽  
Katsumi Tachibana ◽  
Motozumi Nomura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
The Common

Development of an enzyme immunoassay for the detection of hepatitis B surface antigen employing monoclonal antibodies

1993 ◽  
Vol 7 (6) ◽  
pp. 324-328 ◽  
Author(s):  
Emilena Toplikar ◽  
Amadeo Carlomagno ◽  
L. Federico Rojkin ◽  
Rosana Gariglio ◽  
Luis E. Lorenzo
Keyword(s):  
Monoclonal Antibodies ◽  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen

scholarly journals Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

1976 ◽  
Vol 29 (10) ◽  
pp. 873-879 ◽  
Author(s):  
G Wolters ◽  
L Kuijpers ◽  
J Kacaki ◽  
A Schuurs
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen

Solid-phase enzyme immunoassay for hepatitis B surface antigen.

1977 ◽  
Vol 23 (5) ◽  
pp. 813-815 ◽  
Author(s):  
R Wei ◽  
G J Knight ◽  
D H Zimmerman ◽  
H E Bond
Keyword(s):  
Hepatitis B ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Room Temperature ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Solid Support ◽  
Controlled Pore Glass ◽  
Pore Glass

Abstract A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.

A monoclonal-antibody enzyme immunoassay for detection of hepatitis B surface antigen with use of a biotin-avidin system.

1985 ◽  
Vol 31 (2) ◽  
pp. 202-205 ◽  
Author(s):  
Y S Liu ◽  
A Green
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Standard Procedure ◽  
Complex Form ◽  
Antibody Complex ◽  
Covalently Linked ◽  
Viral Determinants

Abstract In this solid-phase two-site enzyme immunoassay for hepatitis B surface antigen (HBsAg), three monoclonal anti-HBs are used: 5D3 (IgM) is immobilized on plastic beads; 5C3 (IgG2a) and 5C 11 (IgG1), labeled with biotin, are used as the first conjugate. Horseradish peroxidase covalently linked to avidin is the second conjugate. First, serum or plasma is incubated with the antibody-coated bead and biotin-labeled antibodies, simultaneously, at 45 degrees C for 1 h ("stat" procedure), 3 h ("standard" procedure), or 18 h ("overnight" procedure), during which HBsAg forms a complex with the solid-phase antibody and the biotinylated antibodies. The enzyme-conjugated avidin is then bound to the biotin on the antigen-antibody complex at 45 degrees C for 15 min ("stat") or 30 min (standard and overnight procedures). The beads are incubated with enzyme-substrate solution (H2O2 and o-phenylenediamine). Color developed is measured at 492 nm. All procedures satisfied third-generation HBsAg tests required by the FDA Office of Biologics, being sensitive both to ad and ay subtypes in subnanogram amounts. The assay is reactive with adw2, adw4, adr, ayw2, ayw3, and ayr subtypes and can detect viral determinants in HBsAg-anti-HBs immune complex form. Thus it provides a sensitive, simple, and reproducible alternative to radioimmunoassay.

Synthesis of hepatitis B surface antigen pre-S2 region fragments and studies on their immunogenicity

1988 ◽  
Vol 53 (11) ◽  
pp. 2952-2956 ◽  
Author(s):  
Bernard Lammek ◽  
Zbigniew Maćkiewicz ◽  
Izabela Derdowska ◽  
Hanna Świderska ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Antigenic Determinants ◽  
Phase Method ◽  
Peptide Fragments ◽  
Humoral Immune

Two peptide fragments of hepatitis B surface antigen pre-S2 region were synthesized by the solid phase method. The peptides were purified by gel filtration or ion-exchange chromatography on Sephadex SP-C-25. Both peptides induced a cellular and humoral immune response in rabbits. The results showed that fragment 14-22 of pre-S2 region contains one of the antigenic determinants.

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