Synthesis of hepatitis B surface antigen pre-S2 region fragments and studies on their immunogenicity

1988 ◽  
Vol 53 (11) ◽  
pp. 2952-2956 ◽  
Author(s):  
Bernard Lammek ◽  
Zbigniew Maćkiewicz ◽  
Izabela Derdowska ◽  
Hanna Świderska ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Antigenic Determinants ◽  
Phase Method ◽  
Peptide Fragments ◽  
Humoral Immune

Two peptide fragments of hepatitis B surface antigen pre-S2 region were synthesized by the solid phase method. The peptides were purified by gel filtration or ion-exchange chromatography on Sephadex SP-C-25. Both peptides induced a cellular and humoral immune response in rabbits. The results showed that fragment 14-22 of pre-S2 region contains one of the antigenic determinants.

Conjugates of Hepatitis B Surface Antigen (14-32)Pre-S2 Region with N-Acetylmuramic Acid, N-Acetylnormuramic Acid, and Their L-Alanyl-D-Isoglutamine Derivatives: Synthesis and Studies on Immunogenicity

1992 ◽  
Vol 57 (1) ◽  
pp. 204-211
Author(s):  
Zbigniev Maćkiewicz ◽  
Hanna Świderska ◽  
Maria Kalmanowa ◽  
Zygfryd Smiatacz ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Phase Method ◽  
Virus Surface ◽  
Solid Phase Method ◽  
Cellular And Humoral Immunity ◽  
Cellular Immune

Four novel analogs of hepatitis B virus surface antigen (14-32)Pre-S2 region fragment attached covalently to N-acetylmuramic acid, N-acetylmuramyl-L-alanyl-D-isoglutamine, N-acetylnormuramic acid, and N-acetylnormuramyl-L-alanyl-D-isoglutamine were synthesized by the solid phase method. The ability of analogs to induce cellular and humoral immunity to native HBsAg was tested on rabbits. Cellular immune response occurred in vitro, and HBs antibodies were detected in all immunized animals. No additional adjuvants were used in the tests.

New technique for solid-phase immunoassay: application to hepatitis B surface antigen.

1979 ◽  
Vol 25 (1) ◽  
pp. 178-182 ◽  
Author(s):  
H Park
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Large Volume ◽  
Room Temperature ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
New Technique ◽  
Sandwich Assay ◽  
Communication Device ◽  
Flow Through

Abstract I describe a new technique for improving the sensitivity of the solid-phase "sandwich" assay, by using the through-passage receptacle in a novel flow-communication device. The technique allows a large volume of serum to flow through the antibody-coated receptacle repeatedly during the incubation and is thus termed the "flow-through large-volume incubation" method. Binding of 125I-labeled hepatitis B surface antigen to its corresponding antibody on a solid-phase by this method was more rapid and persistent than binding by the conventional method. When the method was applied to the first incubation of the sandwich assay, the test for the antigen was rendered four-, eight-, and 32-fold as sensitive as an accepted third-generation test for the antigen, by incubating 5-mL volumes of serum at (a) room temperature for 18 h, (b) 45 degrees C for 8 h, or (c) room temperature for seven days, respectively.

scholarly journals Effect of solid phase radioimmunoassay-bead method to detect hepatitis B surface antigen.

RADIOISOTOPES ◽  
1984 ◽  
Vol 33 (12) ◽  
pp. 887-890
Author(s):  
Akio YOSHIMOTO ◽  
Yoshikatsu SAKAGISHI ◽  
Jean Dean LIU ◽  
Seiei TANAKA
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Solid Phase Radioimmunoassay ◽  
Bead Method

scholarly journals Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

1976 ◽  
Vol 29 (10) ◽  
pp. 873-879 ◽  
Author(s):  
G Wolters ◽  
L Kuijpers ◽  
J Kacaki ◽  
A Schuurs
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen

scholarly journals Immobilization of Antibodies on Ultraflat Polystyrene Surfaces

2000 ◽  
Vol 46 (9) ◽  
pp. 1456-1463 ◽  
Author(s):  
Weiping Qian ◽  
Danfeng Yao ◽  
Fang Yu ◽  
Bin Xu ◽  
Rong Zhou ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Uniform Distribution ◽  
Surface Antigen ◽  
Solid Phase ◽  
Physical Adsorption ◽  
Hepatitis B Surface Antigen ◽  
Wafer Surface ◽  
Force Microscopy ◽  
Immobilized Antibodies ◽  
Solid Phase Immunoassay

Abstract Background: Functional antibody surfaces were prepared on ultraflat polystyrene surfaces by physical adsorption, and the uniform distribution of monoclonal antibodies against hepatitis B surface antigen (anti-HBs) on such surfaces and the presence of dense hepatitis B surface antigen (HBsAg) particles captured by immobilized antibodies were identified. Methods: A model polystyrene film was spin-coated directly onto a silicon wafer surface. Atomic force microscopy was used to directly monitor the immobilization of anti-HBs antibodies and their specific molecular interaction with HBsAg. Enzyme immunoassay was also used to characterize functional antibody surfaces. Results: A mean roughness of 2 Å for areas of 25 μm2 was produced. We found a uniform distribution of anti-HBs antibodies on ultraflat polystyrene surfaces and the presence of dense HBsAg particles bound to such anti-HBs surfaces after incubation with HBsAg. Conclusions: This study confirmed the potential of preparing dense, homogeneous, highly specific, and highly stable antibody surfaces by immobilizing antibodies on polystyrene surfaces with controlled roughness. It is expected that such biofunctional surfaces could be of interest for the development of new solid-phase immunoassay techniques and biosensor techniques.

Solid phase reverse passive hemadsorption test for hepatitis B surface antigen

1987 ◽  
Vol 18 (2-3) ◽  
pp. 107-112 ◽  
Author(s):  
G. Duverlie ◽  
M. Driencourt ◽  
C. Roussel ◽  
P. Daniel
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen
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