Rapid determination of hepatitis B surface antigen (HBsAg) by latex agglutination using an integrating sphere turbidimetric assay

1993 ◽  
Vol 158 (2) ◽  
pp. 251-256
Author(s):  
Masayoshi Yamashiki ◽  
Akira Nishimura ◽  
Hiroshi Kishioka ◽  
Kojiro Takase ◽  
Yoshitane Kosaka
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Rapid Determination ◽  
Hepatitis B Surface Antigen ◽  
Latex Agglutination ◽  
Integrating Sphere ◽  
Turbidimetric Assay

Results of the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen

1976 ◽  
Vol 4 (1) ◽  
pp. 32-39
Author(s):  
R N Taylor ◽  
K M Fulford
Keyword(s):  
Hepatitis B ◽  
Disease Control ◽  
Proficiency Testing ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Latex Agglutination ◽  
Lower Sensitivity ◽  
Passive Hemagglutination ◽  
Testing Program ◽  
Efficiency And Effectiveness

The changes in the types and numbers of tests used by participants in the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen from the beginning of the program in 1971 until October 1975 are analyzed; the implications of these changes are discussed. Changes in the use of agar gel diffusion, rheophoresis, counterelectrophoresis, complement fixation, reverse passive latex agglutination, radioimmunoassay, and reverse passive hemagglutination tests are reviewed. The performance of the participants for 1975 is reported, and factors related to performance (type or combinations of tests used, procedures used to confirm specificity, etc.) are discussed. The increase in the use of third-generation tests in place of tests with lower sensitivity and the decrease in the use of multiple tests undoubtedly represent increased efficiency and effectiveness.

scholarly journals Detection of hepatitis B surface antigen in whole blood by coupled particle light scattering (Copalis™)

1997 ◽  
Vol 43 (9) ◽  
pp. 1764-1770 ◽  
Author(s):  
Michael J Benecky ◽  
Diane R Post ◽  
Susan M Schmitt ◽  
Manish S Kochar
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Whole Blood ◽  
Direct Detection ◽  
Hepatitis B Surface Antigen ◽  
Light Scatter ◽  
Blood Samples ◽  
Cell Components ◽  
Multiple Analytes

Abstract Coupled particlelight scattering (Copalis™) is a homogeneous immunoassay technology that permits simultaneous determination of multiple analytes in serum, plasma, or whole blood. Copalis differentiates monomeric latex microparticles from latex aggregates and cells on the basis of their unique light scatter properties. Copalis readily discriminates small (∼0.1 μm) differences in latex microparticle size. Therefore, multiple simultaneous assays are configured by the use of mixtures of different-size latex microparticles. The Copalis research immunoassay for hepatitis B surface antigen (HBsAg) is configured in a sandwich format where the extent of light scatter histogram broadening due to HBsAg-mediated binding of colloidal gold to latex provides the basis for antigen quantification. Simultaneous Copalis forward- and wide-angle light scatter measurements allow discrimination of latex microparticles from the cell components of whole blood. Consequently, direct detection of HBsAg in unprocessed whole-blood samples by Copalis is feasible.

Determination of the affinity of antibodies to hepatitis B surface antigen in human sera

1984 ◽  
Vol 72 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Sheila E. Brown ◽  
Colin R. Howard ◽  
Arie J. Zuckerman ◽  
Michael W. Steward
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Human Sera

Differential genetic determination of immune responsiveness to hepatitis B surface antigen and to hepatitis A virus: a vaccination study in twins

The Lancet ◽  
2002 ◽  
Vol 360 (9338) ◽  
pp. 991-995 ◽  
Author(s):  
Thomas Höhler ◽  
Esther Reuss ◽  
Nina Evers ◽  
Evi Dietrich ◽  
Christian Rittner ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hepatitis B Surface Antigen ◽  
Immune Responsiveness ◽  
Genetic Determination
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