scholarly journals Detection of hepatitis B surface antigen in whole blood by coupled particle light scattering (Copalis™)

1997 ◽  
Vol 43 (9) ◽  
pp. 1764-1770 ◽  
Author(s):  
Michael J Benecky ◽  
Diane R Post ◽  
Susan M Schmitt ◽  
Manish S Kochar
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Whole Blood ◽  
Direct Detection ◽  
Hepatitis B Surface Antigen ◽  
Light Scatter ◽  
Blood Samples ◽  
Cell Components ◽  
Multiple Analytes

Abstract Coupled particlelight scattering (Copalis™) is a homogeneous immunoassay technology that permits simultaneous determination of multiple analytes in serum, plasma, or whole blood. Copalis differentiates monomeric latex microparticles from latex aggregates and cells on the basis of their unique light scatter properties. Copalis readily discriminates small (∼0.1 μm) differences in latex microparticle size. Therefore, multiple simultaneous assays are configured by the use of mixtures of different-size latex microparticles. The Copalis research immunoassay for hepatitis B surface antigen (HBsAg) is configured in a sandwich format where the extent of light scatter histogram broadening due to HBsAg-mediated binding of colloidal gold to latex provides the basis for antigen quantification. Simultaneous Copalis forward- and wide-angle light scatter measurements allow discrimination of latex microparticles from the cell components of whole blood. Consequently, direct detection of HBsAg in unprocessed whole-blood samples by Copalis is feasible.

scholarly journals Hepatitis B and Malaria Among Nepalese Blood Donors

1970 ◽  
Vol 5 (5) ◽  
pp. 81-84 ◽  
Author(s):  
Prakash Ghimire ◽  
Bishnu Bhakta Dhungyel ◽  
Bishnu Raj Tiwari
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Surface Antigen ◽  
Disease Burden ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Malarial Parasite ◽  
Blood Samples ◽  
Blood Banks ◽  
Donated Blood

Viral hepatitis and malaria, both are the diseases with noticeable disease burden in Nepal. Malaria is seasonal with high disease burden during post rainy season. Severe malaria also shows similar symptoms to viral hepatitis. This is collaborative study has been conducted during June - September 2006 with the objective of determining the prevalence of hepatitis B and malaria in Nepalese blood donors to find out the need of routine malaria testing in each pint of donated blood. During the study period, screening of malaria and hepatitis B surface antigen (HBsAg) were done in 1200 blood samples collected from blood donors at Kathmandu, Nepalgunj and Biratnagar Blood Banks. Malaria diagnosis was done using one drop of blood from each unit of blood using Giemsa stained thick and thin smear microscopy, while hepatitis B surface antigen (HBsAg) was detected using commercial ELISA. Of the total 1200 blood samples analyzed, 600 were collected at Kathmandu and 300 each at Nepalgunj and Biratnagar blood banks, situated in malaria endemic southern districts. Among the total 1200 samples, 1% (12) of the sample was found reactive for HBsAg; while only 0.33 % (4) samples were positive for malarial parasite. 1.33% (8) samples from Kathmandu and 1.33% (4) samples from Nepalgunj were positive for HBV. 1% (3) samples from Nepalgunj and 0.33% (1) sample from Biratnagar were found to be positive for malarial parasite. None of the samples from Biratnagar were positive for HBsAg, while none of the samples from Kathmandu were positive for malarial parasite. All of the malaria cases were due to Plasmodium vivax. All the infected cases were male. Co-infection of malaria and HBV was not observed during the entire period of study. However more extensive study is needed in other endemic areas of Nepal, the study indicated that the continuation of screening each point of donated blood for Hepatitis B, while screening of malarial parasites in donors of endemic terai districts may be useful in preventing transmission of malaria through transfusion. Key words: Hepatitis; Malaria; ELISA; Blood donors; Blood transfusion. DOI: 10.3126/sw.v5i5.2661 Scientific World, Vol. 5, No. 5, July 2007 81-84

Determination of the affinity of antibodies to hepatitis B surface antigen in human sera

1984 ◽  
Vol 72 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Sheila E. Brown ◽  
Colin R. Howard ◽  
Arie J. Zuckerman ◽  
Michael W. Steward
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Human Sera

Differential genetic determination of immune responsiveness to hepatitis B surface antigen and to hepatitis A virus: a vaccination study in twins

The Lancet ◽  
2002 ◽  
Vol 360 (9338) ◽  
pp. 991-995 ◽  
Author(s):  
Thomas Höhler ◽  
Esther Reuss ◽  
Nina Evers ◽  
Evi Dietrich ◽  
Christian Rittner ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hepatitis B Surface Antigen ◽  
Immune Responsiveness ◽  
Genetic Determination

scholarly journals Prevention of mother-to-child transmission of hepatitis B virus in Burkina Faso: Screening, vaccination and evaluation of post-vaccination antibodies against hepatitis B surface antigen in newborns

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Edwige T. Yelemkoure ◽  
Albert T. Yonli ◽  
Carla Montesano ◽  
Abdoul Karim Ouattara ◽  
Birama Diarra ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Pregnant Women ◽  
Vertical Transmission ◽  
Surface Antigen ◽  
Transmission Rate ◽  
Hepatitis B Surface Antigen ◽  
Venous Blood ◽  
Blood Samples ◽  
B Virus

The low rate of screening for hepatitis B virus (HBV) in pregnant women is a highrisk factor for its vertical transmission. The objectives of this study were: i) to screen pregnant women for HBV infection; ii) vaccinate all children from birth against HBV regardless their mother HBV status; and iii) evaluate after 7 months of birth the level of their AbHBs among babies who received HBV vaccine at birth. Serological markers of HBV (HBsAg, HBeAg, AbHBs, AbHBe, and AbHBc) were determined on venous blood samples from 237 pregnant women and their children using the Abon Biopharm Kit. One hundred and two (102) children received the three doses of the EUVAX B® vaccine respectively at birth, two months and four months of life. Seven months after delivery, venous blood samples were collected from mothers and their children. Antibodies against hepatitis B surface antigen (AbHBs) were measured in vaccinated children using the ELISA Kit AbHBs Quantitative EIA. DNA extraction was performed on samples from HBV-seropositive mothers and their children using the Ribo Virus (HBV Real-TM Qual) Kit and for Real Time PCR, the HBV Real-TM Qual Kit was used. Serological diagnosis in pregnant women revealed 22 (9.28%) hepatitis B surface antigen (HBsAg) positive samples of which 21 were positive for viral DNA by real-time PCR. Among the 22 HBsAg+ women, five (05) transmitted the virus to their children with a vertical transmission rate of 22.73%. A transmission rate of 23.81% (5/21) was found with the PCR method. Analysis of AbHBs levels revealed that 98.31% of the children had an average concentration of 218.07 ± 74.66 IU/L, which is well above the minimum threshold for protection (11 IU/L). This study has confirmed that vertical transmission of HBV is a reality in Burkina Faso and that vaccination at birth would significantly reduce this transmission.

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