Specific binding of [3H]ouabain to heart is inhibited by long-chain fatty acids and alcohols

1980 ◽  
Vol 12 (9) ◽  
pp. 847-856 ◽  
Author(s):  
R Kim
Keyword(s):  
Fatty Acids ◽  
Specific Binding ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Fatty Acids And Alcohols ◽  
Chain Fatty Acids

Fit-for-purpose biomarker LC–MS/MS qualification for the quantitation of very long chain fatty acids in human cerebrospinal fluid

Bioanalysis ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 143-158
Author(s):  
John Williams ◽  
Kan Zhu ◽  
Eric Crampon ◽  
André Iffland
Keyword(s):  
Fatty Acids ◽  
Cerebrospinal Fluid ◽  
Specific Binding ◽  
Long Chain Fatty Acids ◽  
Absolute Quantitation ◽  
Peroxisomal Disorders ◽  
Healthy Human ◽  
Fit For Purpose ◽  
Long Chain ◽  
Chain Fatty Acids

Aim: Very long chain fatty acids (VLCFAs) have been identified as biomarkers for several peroxisomal disorders necessitating the need for reliable biomarker assays in particular C20, C22, C24, C26 in cerebrospinal fluid (CSF). Until now no absolute quantitation assay for total VLCFAs in CSF has been successfully developed and qualified for clinical use. Methodology: A quantitative LC–MS/MS assay for total VLCFA in human CSF was developed. Derivatization tag and coupling chemistry were optimized for sensitivity. CSF contamination by blood, non-specific binding of VLCFA to surfaces and exogenous VLCFA contamination was minimized. Discussion/conclusion: This fit for purpose biomarker assay was used to measure baseline healthy human VLCFA levels across multiple subjects to establish an understanding of concentration ranges and feasibility.

Permeation of long-chain fatty acids and alcohols in rat intestine.

1979 ◽  
Vol 236 (6) ◽  
pp. E721
Author(s):  
V L Sallee
Keyword(s):  
Fatty Acids ◽  
Bile Salt ◽  
Saturated Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Salt Solutions ◽  
Lipid Concentration ◽  
Uptake Rates ◽  
Long Chain ◽  
Fatty Acids And Alcohols ◽  
Chain Fatty Acids

Uptake of long-chain fatty acids and alcohols from micellar bile salt solutions has been studied with everted sacs of rat jejunum. These unidirectional uptake rates are linearly related to lipid concentration if bile salt concentration is constant, but are essentially independent of lipid concentration over a 2.5-fold concentration range if lipid and bile salt concentrations are maintained at a constant ratio. Uptake of lipid from various micellar bile salt solutions was directly related to the experimentally varied monomer activity of solute, thus allowing conversion of uptake rates to permeation coefficients. Natural logarithm of permeation coefficients (ln P) increased linearly for saturated fatty acids of 12--18 carbons, equivalent to an incremental free energy of -695 cal.mol-1 per --CH2--. Alcohols of 10- to 14-carbon chain lengths had a similar relationship on ln P to number of carbons. Previously determined permeation coefficients for fatty acids of 2--10 carbons are now seen to be a nonlinear portion of the curve for ln P versus chain length for all saturated fatty acids.

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