Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli

1971 ◽  
Vol 62 (2) ◽  
pp. 411-414 ◽  
Author(s):  
G. Stöffler ◽  
L. Daya ◽  
K.H. Rak ◽  
R.A. Garrett
Keyword(s):  
Escherichia Coli ◽  
Protein Binding ◽  
Binding Sites ◽  
Ribosomal Proteins ◽  
Specific Protein ◽  
Protein Binding Sites

EVALUATION OF TISSUE STEROID BINDING IN VITRO

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S223-S246 ◽  
Author(s):  
C. R. Wira ◽  
H. Rochefort ◽  
E. E. Baulieu
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Experimental System ◽  
Specific Protein ◽  
Coupling Mechanism ◽  
Target Tissue ◽  
Protein Binding Sites ◽  
Definition Of ◽  
Hormone Specificity

ABSTRACT The definition of a RECEPTOR* in terms of a receptive site, an executive site and a coupling mechanism, is followed by a general consideration of four binding criteria, which include hormone specificity, tissue specificity, high affinity and saturation, essential for distinguishing between specific and nonspecific binding. Experimental approaches are proposed for choosing an experimental system (either organized or soluble) and detecting the presence of protein binding sites. Techniques are then presented for evaluating the specific protein binding sites (receptors) in terms of the four criteria. This is followed by a brief consideration of how receptors may be located in cells and characterized when extracted. Finally various examples of oestrogen, androgen, progestagen, glucocorticoid and mineralocorticoid binding to their respective target tissues are presented, to illustrate how researchers have identified specific corticoid and mineralocorticoid binding in their respective target tissue receptors.

scholarly journals Maximal Activity of an Erythroid-specific Enhancer Requires the Presence of Specific Protein Binding Sites in Linked Promoters

1998 ◽  
Vol 273 (22) ◽  
pp. 13593-13598 ◽  
Author(s):  
Persis J. Amrolia ◽  
Wesley Gabbard ◽  
John M. Cunningham ◽  
Stephen M. Jane
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Maximal Activity ◽  
Specific Protein ◽  
Protein Binding Sites

scholarly journals Identification of regulatory sequences and protein-binding sites in the liver-type promoter of a gene encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

1991 ◽  
Vol 11 (2) ◽  
pp. 1099-1106 ◽  
Author(s):  
F P Lemaigre ◽  
S M Durviaux ◽  
G G Rousseau
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Specific Protein ◽  
Regulatory Sequences ◽  
Alternative Promoters ◽  
Gene Encoding ◽  
Protein Binding Sites ◽  
Cis Acting ◽  
Dnase I Footprinting ◽  
Nuclear Extracts

The liver-type and muscle-type isozymes of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase are encoded by one gene that uses two alternative promoters. We have identified cis-acting sequences and protein-binding sites on the liver-type promoter. Transfection assays with deleted promoters showed that maximal promoter activity is contained within 360 bp upstream of the cap site. DNase I footprinting experiments with liver and spleen nuclear extracts and with purified proteins revealed several protein-binding sites in this region. These included four binding sites for nuclear factor I, one site that contains an octamer consensus but showed a liver-specific footprint pattern, two liver-specific protein-binding sites, and one poly(dG)-containing binding site. Transfection of cells of hepatic origin suggested that all these sites except one are involved in transcriptional regulation. The region between -360 and -2663 contained an element that functioned as a silencer in a nonhepatic cell line. We conclude that in liver transcription from the liver-type promoter of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene is controlled by ubiquitous and tissue-specific factors and involves activating and derepressing mechanisms.

PROTEIN-RNA INTERACTIONS IN BACTERIAL RIBOSOMES

1973 ◽  
Vol 74 (Suppl) ◽  
pp. S75-S94 ◽  
Author(s):  
R. A. Garrett ◽  
H. G. Wittmann
Keyword(s):  
Protein Binding ◽  
Structural Properties ◽  
Conformational Changes ◽  
Binding Sites ◽  
Ribosomal Proteins ◽  
Structural Factors ◽  
Optimal Conditions ◽  
Protein Binding Sites ◽  
E Coli ◽  
Experimental Approaches

ABSTRACT There are several ribosomal proteins which can bind specifically to the three ribosomal RNA's of E. coli. Some structural properties of these proteins and of the RNA are described together with the optimal conditions for binding. Evidence for conformational changes occurring in some proteins, and in the RNA's during binding, is also discussed. The partial localisation of protein binding sites on the 16S and 5S RNA's, and for one protein on the 23S RNA has been attained recently. However, relatively little is known about the regions of the protein which interact with the RNA. Although the nature of the specificity of the protein-RNA interactions is not yet understood, some experimental approaches which are in progress to elucidate the basis of this specificity are mentioned together with a discussion of the structural factors which may be important.

scholarly journals Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli

1975 ◽  
Vol 2 (2) ◽  
pp. 279-302 ◽  
Author(s):  
R.A. Zimmermann ◽  
G.A. Mackie ◽  
A. Muto ◽  
R.A. Garrett ◽  
E. Ungewickell ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Binding ◽  
Ribosomal Protein ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
16S Rna
Sign in / Sign up

Export Citation Format

Share Document