Influence of age and sex on the metabolism of oestrogens in mouse liver: in vitro perfusion of radioactive oestrone and oestradiol-17β

SummaryHeparin is known to affect platelet function in vitro, but little is known about the effect of heparin on the interaction of platelets with polymer surfaces in general, and vascular graft materials in particular. For this reason, the effect of heparin vs. citrate anticoagulation on the interaction of platelets with the vascular graft materials expanded polytetrafluoroethylene (ePTFE), Dacron Bionit (DB) and preclotted Dacron Bionit (DB/PC) was studied in a recirculating, in vitro perfusion system. Platelet activation, as shown by a decrease in platelet count, an increase in platelet release and a decrease in platelet aggregation, was observed for all vascular graft materials tested using heparin and was greater for Dacron and preclotted Dacron than for ePTFE. Significant differences between heparin and citrate anticoagulation were seen for platelet release, platelet aggregation and the relative ranking of material platelet-reactivity. However, the trends and time course of platelet activation were similar with both heparin and citrate for the materials tested.

Download Full-text

Der Effekt von Hypoxie auf die plazentare Leptinsynthese in der dualen in-vitro Perfusion humaner Plazenten

Zeitschrift für Geburtshilfe und Neonatologie ◽

10.1055/s-2003-818188 ◽

2004 ◽

Vol 207 (S 2) ◽

Author(s):

S Kuno ◽

K May ◽

R Trollmann ◽

J Dötsch ◽

N Siebert ◽

...

Keyword(s):

In Vitro Perfusion

Download Full-text

Apoprotein C effect on triglyceride transport in tandem-perfused rat hind end and liver

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1984.247.3.g305 ◽

1984 ◽

Vol 247 (3) ◽

pp. G305-G310

Author(s):

W. J. Kortz ◽

J. R. Nashold ◽

M. R. Greenfield ◽

H. Hilderman ◽

S. H. Quarfordt

Keyword(s):

Fatty Acid ◽

Free Fatty Acid ◽

Fatty Acyl ◽

Liver Fat ◽

Molar Ratio ◽

Perfusion System ◽

In Vitro Perfusion ◽

Arterial Inflow ◽

High Concentrations

The metabolism of double-labeled triglyceride in a synthetic emulsion was defined in an in vitro perfusion system of rat hind end and liver described previously [Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G106-G112, 1983]. The metabolism of [3H]glycerol-[14C]triolein was defined in the absence of added apoproteins and with additions of human CII and both CII and CIII. Without apoprotein, a pronounced lipolysis of the triglyceride was recognized by high concentrations of radiolabeled glycerol and free fatty acid in the perfusate. The removal of an aliquot of hind-end venous effluent 5 min after adding the labeled triglyceride emulsion to the arterial inflow demonstrated a brisk lipolysis of the substrate when incubated outside the perfusion system. The addition of CII protein to the emulsion before its introduction into the tandem system eliminated perfusate lipolysis, both within the perfusion system and in incubations of aliquots withdrawn from the system. Intravascular lipolysis was not seen with triglyceride emulsions containing both CII and CIH or when an aliquot of hind-end venous effluent was incubated with triglycerides that had not been exposed to the perfusion system. The intravascular lipolysis observed for the [14C]triglyceride added to the tandem system without apoproteins was associated with relatively greater recoveries of 14C-fatty acyl in liver, fat, and muscle and relatively greater recoveries of 14CO2 than when CII alone or both CII and CIII were added with the triglyceride. The addition of CIII to CII in a 1:1 molar ratio increased the recovery of 14C-fatty acyl in muscle and the recovery as 14CO2.(ABSTRACT TRUNCATED AT 250 WORDS)

Download Full-text

In Vitro Production of Erythropoietin by Kidneys Perfused With a Serum-free Solution

Blood ◽

10.1182/blood.v44.1.77.77 ◽

1974 ◽

Vol 44 (1) ◽

pp. 77-85 ◽

Cited By ~ 57

Author(s):

Allan J. Erslev

Keyword(s):

Tissue Culture ◽

Atmospheric Pressure ◽

Culture Medium ◽

Kidney Tissue ◽

In Vitro Production ◽

In Vitro Perfusion ◽

Serum Free ◽

Enzymatic Activation ◽

Vitro Production

Abstract Normal rabbits exposed to 0.4 atmospheric pressure for 3 hr will generate about 40-60 U of erythropoietin during a subsequent 3-hr period. If the kidneys were removed from 3-hr hypoxic animals, washed carefully, and perfused for 3 hr by recirculation with a serum-tissue culture mixture, each kidney generated about 14 U of erythropoietin in vitro. Perfusion of normal kidneys did not result in the production of erythropoietin, and only small amounts were generated if the perfusate contained Puromycin. Three-hour hypoxic kidneys perfused for 3 hr with a serum-free tissue culture medium were found to generate about 8 U of erythropoietin per kidney and similar kidneys perfused with saline about 1 U. These results indicate that erythropoietin is synthesized by kidney tissue and not produced by enzymatic activation of a plasma substrate.

Download Full-text