Theory of cell age distribution in suspension culture with feed and / or drain

1990 ◽  
Vol 99 (1) ◽  
pp. 141
Author(s):  
Hiroumi Funakoshi ◽  
Akio Yamada
1989 ◽  
Vol 13 (3) ◽  
pp. 283-289 ◽  
Author(s):  
A CUADRADO ◽  
M NAVARRETE ◽  
J CANOVAS

1977 ◽  
Vol 23 (8) ◽  
pp. 1409-1415 ◽  
Author(s):  
K B Woo ◽  
R D Enagonio

Abstract A quantitative relationship between tumor cell number and biologic marker concentration has been investigated and characterized by the previously developed discrete-time kinetic model for the study of cell kinetics. Here, this model is further expanded to cope with both cell cycle kinetics and biologic marker synthesis. A synchronized tumor-cell population is examined to determine the time course of the synthesis of markers in relation to cell cycle. The DNA content distributions, measured by flow microfluorometry, are analyzed by use of the model, and the cell age distribution is extracted. The average marker content per cell in cell cycle is measured for each time sequence in synchronized cell populations. The model, incorporating the cell age distribution and the average marker content per cell in cell cycle, enables one to generate the marker content distribution, from which the cell number is estimated from the marker concentration. The model performance is further evaluated with Chinese hamster ovary cells, and their polyamine content and the total polyamine concentration during synchronization is calculated and related to the total cell number.


2007 ◽  
Vol 212 (3-4) ◽  
pp. 347-357 ◽  
Author(s):  
Joaquín Martí ◽  
M. C. Santa-Cruz ◽  
Shirley A. Bayer ◽  
Bernardino Ghetti ◽  
José P. Hervás

1996 ◽  
Vol 37 (4) ◽  
pp. 556-556
Author(s):  
Tetsuo Murakami ◽  
Kumiko Takemori ◽  
Hajime Yoshizumi ◽  
Hiroyuki Ito

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