Alphafetoprotein (AFP), concanavalin A non-reactive AFP and specific acetylcholinesterase in amniotic fluid from pathological pregnancies. Predictive values for open spina bifida

1983 ◽  
Vol 16 (1) ◽  
pp. 9-18 ◽  
Author(s):  
N. van Regemorter ◽  
V. Defleur ◽  
D. Delbeke ◽  
E. Vamos ◽  
F. Rodesch
Keyword(s):  
Spina Bifida ◽  
Amniotic Fluid ◽  
Concanavalin A ◽  
Predictive Values ◽  
Open Spina Bifida

Concanavalin A binding of alpha-fetoprotein in amniotic fluid as an aid in the diagnosis of neural tube defects.

1981 ◽  
Vol 27 (10) ◽  
pp. 1658-1660 ◽  
Author(s):  
P K Buamah ◽  
P Taylor ◽  
A M Ward
Keyword(s):  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Concanavalin A ◽  
Gestational Age ◽  
Alpha Fetoprotein ◽  
Normal Outcome ◽  
Fetal Malformations ◽  
Open Spina Bifida

Abstract Concanavalin A nonreactive alpha-fetoprotein was determined in samples of amniotic fluid from 16 abnormal pregnancies complicated by anencephaly (7), open spina bifida (6), intra-uterine death (1), anencephaly with exomphalos (1), or open spina bifida with exomphalos (1), and in amniotic fluid from 50 normal pregnancies with gestational age between 13 and 24 weeks. In all 16 cases with fetal malformations, the proportion of nonreactive alpha-fetoprotein was significantly decreased (median 5.3%) as compared with amniotic fluid from pregnancies with a normal outcome (median 39.7%). The results confirm that this measurement is useful in the diagnosis of neural tube defects, especially when the concentration of alpha-fetoprotein in amniotic fluid is normal or only slightly above normal and gestational age is uncertain.

scholarly journals Affinity Chromatography of Amniotic Fluid Alphafetoprotein Using Concanavalin A-Sepharose

1981 ◽  
Vol 18 (6) ◽  
pp. 350-352 ◽  
Author(s):  
Jennifer A Noyes ◽  
P J Wood
Keyword(s):  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defect ◽  
Concanavalin A ◽  
Con A ◽  
Potential Value ◽  
Significant Difference ◽  
Normal Outcome ◽  
Chromatography Columns

We measured Concanavalin A (Con A) non-binding alphafetoprotein in amniotic fluid from 21 normal pregnancies and 20 abnormal pregnancies (complicated by anencephaly, spina bifida, or exomphalos) using small Con A-sepharose chromatography columns. There was a highly significant difference between percentage non-binding alphafetoprotein levels for pregnancies with a normal outcome (mean result 32%; range 18–47%) and pregnancies complicated by a neural tube defect or exomphalos (mean 14%; range 7–20%). The test is therefore of potential value in cases where there is uncertainty over the interpretation of total amniotic fluid alphafetoprotein levels.

scholarly journals Neurone Specific Enolase in Amniotic Fluid: A Potential Marker of Anencephaly

1988 ◽  
Vol 25 (1) ◽  
pp. 85-88 ◽  
Author(s):  
K Spencer ◽  
P Carpenter
Keyword(s):  
Prenatal Diagnosis ◽  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Normal Values ◽  
Potential Marker ◽  
Neuronal Proteins ◽  
Non Gaussian ◽  
Open Spina Bifida

Normal values for neurone specific enolase in amniotic fluid have been found to follow a non gaussian distribution with a 1–99 centile range of 1·10–4·32 μg/L. Neurone specific enolase levels have been shown to be raised in the amniotic fluid of pregnancies complicated by anencephaly, although not those complicated by open spina bifida. Neurone specific enolase measured by radioimmunoassay is capable of totally discriminating between normal pregnancies and those complicated by anencephaly. The study demonstrates the possible value of investigating other neuronal proteins which may find value as adjuncts to amniotic fluid Alpha fetoprotein levels in the prenatal diagnosis of Neural Tube Defects.

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