Genetic control of chalcone synthase activity in flowers of Antirrhinum majus

1982 ◽  
Vol 21 (9) ◽  
pp. 2231-2234 ◽  
Author(s):  
Regine Spribille ◽  
Gert Forkmann
Keyword(s):  
Genetic Control ◽  
Chalcone Synthase ◽  
Antirrhinum Majus

Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3′-Hydroxylase Activity in Antirrhinum majus (Snapdragon)

1981 ◽  
Vol 36 (5-6) ◽  
pp. 411-416 ◽  
Author(s):  
G. Forkmann ◽  
G. Stotz
Keyword(s):  
Genetic Control ◽  
Microsomal Fraction ◽  
Hydroxyl Group ◽  
Antirrhinum Majus ◽  
Clear Correlation ◽  
Soluble Enzyme ◽  
Hydroxylase Activity ◽  
Anthocyanin Pathway

Abstract In flower extracts of defined genotypes of Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation of naringenin and eriodictyol in the 3-position and of naringenin in the 3′-position. The 3-hydroxylase is a soluble enzyme and belongs according to its cofactor requirement to the 2-oxoglutarate-dependent dioxygenases. Investigations on different genotypes revealed a clear correlation between block of the anthocyanin pathway by recessive alleles of the gene inc and a complete lack of 3-hydroxylase activity. Chemogenetic studies on different genotypes suggested that the 3′-hydroxyl group of the B-ring of flavonoids is introduced at the stage of C15 intermediates. The corresponding 3′-hydroxylase was found to be localized in the microsomal fraction and required NADPH as cofactor. In confirmation of the chemogenetic studies, a strict correlation was found between 3′-hydroxylase activity and the gene eos which is known to control the hydroxylation of flavones, flavonols and anthocyanins in the 3′-position. These results are similar to those previously obtained with Matthioia incana.

Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus

Science ◽  
1990 ◽  
Vol 250 (4983) ◽  
pp. 931-936 ◽  
Author(s):  
Z. Schwarz-Sommer ◽  
P. Huijser ◽  
W. Nacken ◽  
H. Saedler ◽  
H. Sommer
Keyword(s):  
Genetic Control ◽  
Flower Development ◽  
Antirrhinum Majus ◽  
Homeotic Genes

Homeotic Genes in the Genetic Control of Flower Morphogenesis in Antirrhinum majus

Development ◽  
1992 ◽  
pp. 242-256 ◽  
Author(s):  
Zsuzsanna Schwarz-Sommer ◽  
Heinz Saedler ◽  
Hans Sommer
Keyword(s):  
Genetic Control ◽  
Antirrhinum Majus ◽  
Homeotic Genes ◽  
Flower Morphogenesis

The paramutagenic line niv-44 has a 5 kb insert, Tam 2, in the chalcone synthase gene of Antirrhinum majus

1985 ◽  
Vol 199 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Kailash C. Upadhyaya ◽  
Hans Sommer ◽  
Enno Krebbers ◽  
Heinz Saedler
Keyword(s):  
Chalcone Synthase ◽  
Antirrhinum Majus ◽  
Chalcone Synthase Gene

scholarly journals UV-inducible transient expression in parsley protoplasts identifies regulatory cis -elements of a chimeric Antirrhinum majus chalcone synthase gene

1988 ◽  
Vol 7 (13) ◽  
pp. 4027-4033 ◽  
Author(s):  
Susanne Lipphardt ◽  
Reinhold Brettschneider ◽  
Fritz Kreuzaler ◽  
Jeff Schell ◽  
Jeffery L. Dangl
Keyword(s):  
Transient Expression ◽  
Chalcone Synthase ◽  
Antirrhinum Majus ◽  
Cis Elements ◽  
Chalcone Synthase Gene

Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R.Br

1981 ◽  
Vol 36 (7-8) ◽  
pp. 619-624 ◽  
Author(s):  
R. Spribille ◽  
G. Forkmann
Keyword(s):  
Enzyme Activity ◽  
Genetic Control ◽  
Chalcone Synthase ◽  
Condensation Reaction ◽  
Chalcone Isomerase ◽  
Wild Type ◽  
Enzyme Preparations ◽  
Matthiola Incana ◽  
Anthocyanin Pathway ◽  
Malonyl Coa

Abstract Chalcone synthase activity was demonstrated in enzyme preparations from flowers of defined genotypes of Matthiola incana (stock). The product formed from 4-coumaroyl-CoA and malonyl-CoA was naringenin and not the isomeric chalcone, because chalcone isomerase was also present in the reaction mixture. Chalcone synthase activity could be detected only in flower extracts of genotypes with wild-type alleles at the locus f Thus, the interruption of the anthocyanin pathway in white flowering lines with recessive alleles (ff) of this gene is clearly due to a lack of this enzyme activity. Independent on the genetic state of the locus b which controls the formation of pelargonidin or cyanidin, respectively, in the flowers, 4-coumaroyl-CoA was the only suitable substrate for the condensation reaction.

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