The Effect of Foliar Thiamine and Roselle Extract on NPK Content and Some Chemical Parameters inof Mathiola Plant

The experiment was conducted in the Directorate of Agriculture in Najaf during the agricultural season 2020-2021 to know the effect of Foliar application of thiamine and Roselle extract on the chemical parameters of the Mathiola plant(Matthiola incana L).The experiment included two factors, the first is thiamine at four concentrations (90,60,30,0) mg.L-1. The second is Roselle extract at four concentrations (0,2,4,6) g.L-1, a factorial experiment was conducted consisting of three replicates, the experiment was conducted based on the Randomized Complete Block Design (R.C.B.D) 4×4×3=48, The results can be summarized that thiamine at a concentration of 90 mg.L-1 with Roselle extract at a concentration of 6.4 g.L-1 when sprayed on the plant led to a significant increase in the study parameters as the leaves content of total chlorophyll and nitrogen phosphorous potassium, boron and the total soluble carbohydrate content of the leaves and the anthocyanin content of the flowers were increased reached to 57.27 mg.100 g-1 and 2.45% and 0.29% and 2.96% and 13.72 mg.kg-1 and 18.10 mg.g-1 respectively.

Morphological And Molecular Identification of Double Flowered Stock (Matthiola Incana R. Br) Cultivars With High Fertility

Garden stock (Matthiola incana R. Br.) is a commercially important horticultural crop owing to its ornamental effect. There are different stock cultivars varied in color and shape, especially flowered phenotype is an essential index evaluating its commercial value, because double flowered cultivars have more brilliant flowers compared to single flowered one. The present work aimed: (1) to make superior cultivars with different colors, high fertility, being capable of early selecting only double flowered seedlings by leaf color and to investigate morphological characteristics and (2) to select RAPD and ISSR primers for the cultivar certification and identification to culture and produce good commercial stock cultivars. Here we obtained new double flowered stock cultivars with different colors including pink, pale pink and white, through outcrossing between “white” cultivar (high fertile but unable to select double flower phenotype) and “pink” cultivar (vice. versa). Among newly obtained stock cultivars, single and double flower seedlings are distinguishable from each other by leaf color, having about 70% of fertility. Moreover RAPD and ISSR markers selected in this study can be applied to identify different stock cultivars in seed production, culture and to establish cultivar certification system.

First report on clover proliferation phytoplasma related strain associated with Matthiola incana floral virescence in Uttar Pradesh, India

Matthiola incana R. Br. (Fam: Brassicaceae) is an ornamental, commonly known as hoary stock has an extremely fragrant flowers, which blooms in dense clusters in a large variety of colors. During a survey of flower nurseries in March 2019 at Indian Institute of Sugarcane Research campus, Lucknow, floral virescence (MiV) symptoms (Fig. 1 A, B) were observed in M. incana pots with an incidence of over 40%. Leaf yellows symptoms were also observed on a weed Acalypha indica (AiLY) in Matthiola nursery (Fig. 1 C). Nested PCR assays were carried out to detect and identify the possible association of phytoplasmas with MiV and AiLY symptoms. Three each of symptomatic MiV and AiLY samples and two non-symptomatic samples were collected and processed for DNA extraction from the leaf midrib by CTAB method. Hishimonus phycitis (HP) (Hemiptera: Cicadellidae) leafhopper feeding on MiV symptomatic plants was also collected and DNA was extracted. The DNA of 8 symptomatic and 4 non-symptomatic plants and from the 10 leafhopper was used as a template for PCR assays. Phytoplasma specific 16Sr RNA gene specific primers (P1/P7 and 3Far/3Rev; Schneider et al. 1995; Manimekalai et al. 2010) and multilocus genes’ specific primer pairs for secA (SecAfor1/SecArev3;SecAfo5r/SecARev2; Bekele et al. 2011), secY (SecYF1(VI)/SecYR1(VI);SecYF2(VI)/SecYR1(VI); Lee et al. 2010) and rp genes (rpFIC/rp(I)R1A; rp(VI)F2/ rp(VI)R2; Martini et al. 2007) were employed as previously described. Amplified products of ~1.3kb, ~600bp, ~1.7kb and ~1.0kb of 16S rRNA, secA, secY and rp genes of phytoplasma were consistently amplified in all the MiV and AiLY samples and in the HP leafhopper. No amplifications were achieved in any of the asymptomatic plant samples. Amplified products of all the four genes of MiV, AiLY and HP isolates were purified, sequenced and submitted in GenBank. Sequence comparison and phylogeny analysis of the sequences of the four genes of MiV, AiLY and HP isolates revealed 99% - 100% sequence identity and clustering with clover proliferation phytoplasma related strains (16SrVI group)(Fig.2 A,B,C and D). The virtual RFLP analysis of 17 restriction endonucleases corresponding to the 16S rDNA sequence of MiV, AiLY and HP phytoplasma strains by pDraw program, assigned them into a novel phytoplasma subgroup strain under 16SrVI group, since its HpaII restriction profile was different to earlier classified 16SrVI subgroups but was very close to16SrVI-E subgroup (GenBank acc. no. AY270156) (Fig 3). Earlier, peanut witches’ broom (16SrII-A) phytoplasma was identified associated with M. incana from Italy (Davino et al. 2007). However, the association of clover proliferation phytoplasma (16SrVI) related strain associated with virescence symptom of M. incana is the first report in world. The weed (A. indica) and HP leafhopper were also reported as additional hosts of 16SrVI subgroup related new strain in India, which needs further investigation. The report of a new host and new subgroup of clover proliferation phytoplasma related strain in India is having an epidemiological significance and warrants attention.

Cytotoxic, Antioxidant, and Enzyme Inhibitory Properties of the Traditional Medicinal Plant Matthiola incana (L.) R. Br.

Matthiola incana (L.) R. Br. (Brassicaceae) is widely cultivated for ornamental purposes and utilized as a medicinal plant. In the present work, the hydroalcoholic extract from the aerial parts of this species has been evaluated in different bioassays in order to detect potential pharmacological applications. The cytotoxic capacity against the human colorectal adenocarcinoma (CaCo-2) and breast cancer (MCF-7) cell lines was tested using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The extract was investigated as a neuroprotective inhibitor of central nervous system (CNS) enzymes such as monoamine oxidase A, tyrosinase, acetylcholinesterase, and as a natural enzyme inhibitor of α-glucosidase and lipase involved in some metabolic disorders such as obesity or type 2 diabetes. The antioxidant ability was also evaluated in an enzymatic system (xanthine/xanthine oxidase assay). Results showed that the M. incana extract displayed moderate to low cytotoxicity vs. CaCo-2 cells. The extract acted as a superoxide radical scavenger and enzymatic inhibitor of monoamine oxidase A, tyrosinase, α-glucosidase, and lipase. The best results were found in the α-glucosidase assay, as M. incana hydroalcoholic extract was able to inhibit the enzyme α-glucosidase up to 100% without significant differences, compared to the antidiabetic drug acarbose. Matthiola incana has been demonstrated to exert different biological properties. These are important in order to consider this species as a source of bioactive compounds.

Caracteres morfológicos e identificación de subfases durante la imbibición en semillas de alhelí (Matthiola incana)

Objetivo: Describir los atributos morfologicos y etapas de imbibición en semillas de tres accesiones de Matthiola incana. Diseño/metodología/aproximación: Fueron analizadas semillas de los genotipos Nieve, Rosita y Uva correspondientes genero Matthiola especie incana. En una primera evaluación se calculó el peso de mil semillas y, determino el color de las mismas. A través del procesamiento de imágenes digitales se determinó el área, perímetro, longitud (eje mayor), ancho, ángulo, elongación (longitud/ancho) y el grado de redondez; datos que fueron analizados en una prueba de comparación de medias por Tukey. En la imbibición se colocaron en remojo muestras de cincuenta semillas de cada genotipo y fueron evaluadas cada 24, 48, 72 y 164 h para conocer su dinámica de absorción y poder determinar el tiempo en que transcurren las sub y fases de imbibición. Resultados: La descripción física en semillas de Nieve muestra que son de color naranja moderado y el peso de mil semillas es de 0.218 g; en Rosita la semilla es de color amarillo anaranjado moderado y mil semillas pesan 0.1902; el color en semillas de Uva es grisáceo a café obscuro con un peso de 0.1272 g a mil semillas. En las dimensiones morfológicas Nieve muestra mayores dimensiones respecto a Rosita y Uva; pero entre los tres genotipos muestran semejanzas en el tipo de forma. Durante la prueba de imbibición cada genotipo sigue su ritmo hasta la fase tres, donde el comportamiento se sincroniza en la visión de la protuberancia radicular a las 164 h de iniciada la prueba. Limitaciones del estudio/implicaciones: Las semillas muestran diferente tamaño, por lo cual manipular muestras grandes se complica al momento de la imbibición. Hallazgos/conclusiones: De las tres accesiones se determina que semillas correspondientes a la especie incana, son de mayores atributos morfológicos que otras especies del mismo género. En la fase dos, se identificaron dos subfases, la primera reconocida como el momento de expulsión de solutos y la segunda como recuperación a los solutos perdidos.