Non-uniform staining of myofibrila bands by a monoclonal antibody to skeletal muscle myosin S1 heavy chain

1983 ◽  
Vol 15 (3) ◽  
pp. 341-349 ◽  
Author(s):  
P. Wachsberger ◽  
L. Lampson ◽  
F.A. Pepe
1989 ◽  
Vol 37 (10) ◽  
pp. 1539-1543 ◽  
Author(s):  
J A Sawchak ◽  
J H Sher ◽  
S A Shafiq

We report the characterization of monoclonal antibody (MAb) ALD 180, prepared against the myosin of slow avian muscle, for studies of human muscle development and disease. With the use of radioimmunoassays, Western immunoblots of native and denatured myosins, and epifluorescent indirect immunocytochemistry, we show that ALD 180 is specific for an epitope in human prenatal skeletal muscle myosin heavy chain (MHC), which is expressed in diminishing abundance in fetal fibers from at least 19-22 weeks' gestation to term and also in regenerating muscle fibers seen in diseased muscles from both children and adults. ALD 180 recognizes an epitope apparently different from those reacting with anti-prenatal human myosin MAb previously described, and therefore affords a complementary reagent for use in future studies of human myosin isoform expression and regulation.


2004 ◽  
Vol 96 (4) ◽  
pp. e103-e110 ◽  
Author(s):  
Youri E.C. Taes ◽  
Marijn Speeckaert ◽  
Evelien Bauwens ◽  
Marc R. De Buyzere ◽  
Johan Libbrecht ◽  
...  

2016 ◽  
Vol 37 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Kevin A. Murach ◽  
James R. Bagley ◽  
Kathryn A. McLeland ◽  
Jose A. Arevalo ◽  
Anthony B. Ciccone ◽  
...  

2002 ◽  
Vol 87 (2) ◽  
pp. 182-186 ◽  
Author(s):  
Espen Spangenburg ◽  
Robert Talmadge ◽  
Timothy Musch ◽  
Pfeifer P. ◽  
Richard McAllister ◽  
...  

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