scholarly journals A method for increasing the sensitivity of the hemagglutination-inhibition test with equine influenza virus

Virology ◽  
1963 ◽  
Vol 21 (4) ◽  
pp. 665-666 ◽  
Author(s):  
Byron S. Berlin ◽  
James L. McQueen ◽  
Elva Minuse ◽  
Fred M. Davenport
2014 ◽  
Vol 56 (6) ◽  
pp. 487-492 ◽  
Author(s):  
Lucas Gaíva E Silva ◽  
Alice Mamede Costa Marques Borges ◽  
Eliana Monteforte Cassaro Villalobos ◽  
Maria do Carmo Custodio Souza Hunold Lara ◽  
Elenice Maria Siquetin Cunha ◽  
...  

The prevalence of antibodies against Equine Influenza Virus (EIV) was determined in 529 equines living on ranches in the municipality of Poconé, Pantanal area of Brazil, by means of the hemagglutination inhibition test, using subtype H3N8 as antigen. The distribution and possible association among positive animal and ranches were evaluated by the chi-square test, spatial autoregressive and multiple linear regression models. The prevalence of antibodies against EIV was estimated at 45.2% (95% CI 30.2 - 61.1%) with titers ranging from 20 to 1,280 HAU. Seropositive equines were found on 92.0% of the surveyed ranches. Equine from non-flooded ranches (66.5%) and negativity in equine infectious anemia virus (EIAV) (61.7%) were associated with antibodies against EIV. No spatial correlation was found among the ranches, but the ones located in non-flooded areas were associated with antibodies against EIV. A negative correlation was found between the prevalence of antibodies against EIV and the presence of EIAV positive animals on the ranches. The high prevalence of antibodies against EIV detected in this study suggests that the virus is circulating among the animals, and this statistical analysis indicates that the movement and aggregation of animals are factors associated to the transmission of the virus in the region.


2006 ◽  
Vol 132 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
Adam Meijer ◽  
Arnold Bosman ◽  
Esther E.H.M. van de Kamp ◽  
Berry Wilbrink ◽  
Mirna Du Ry van Beest Holle ◽  
...  

1969 ◽  
Vol 15 (2) ◽  
pp. 203-207 ◽  
Author(s):  
John R. Polley

For use in the hemagglutination–inhibition test, influenza virus antigens in the form of (a) untreated and inactivated allantoic fluids and (b) untreated and inactivated purified virus suspensions were compared. The inactivation procedures used were formaldehyde treatment and gamma irradiation. It was found that there was no significant difference in the potency and specificity of the purified virus antigens but that the formaldehyde-treated allantoic fluids lost potency during this process. The inactivated antigens can be lyophilized for stable storage and, after reconstitution, can be used in the diagnostic laboratory without risk of infection or interference with isolation procedures. For ease and speed of treatment, gamma irradiation is superior to treatment with formaldehyde.


1977 ◽  
Vol 30 (2) ◽  
pp. 88-90
Author(s):  
YOSHIAKI NARUI ◽  
A. TADOKORO ◽  
Y. KIHARA ◽  
S. KIMURA ◽  
K. KUSAGAWA ◽  
...  

2000 ◽  
Vol 34 (2) ◽  
pp. 204-205 ◽  
Author(s):  
Fernando C Motta ◽  
Maristela O Luiz ◽  
José NSS Couceiro

The circulation of influenza C viruses in Rio de Janeiro, Brazil, was studied when significant levels of antibodies were detected (56.7%) with hemagglutination inhibition test, used as a standard methodology for influenza virus studies.


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