scholarly journals Ether treatment of type B influenza virus antigen for the hemagglutination inhibition test.

1981 ◽  
Vol 13 (1) ◽  
pp. 54-57 ◽  
Author(s):  
A S Monto ◽  
H F Maassab
Keyword(s):  
Influenza Virus ◽  
Hemagglutination Inhibition ◽  
Virus Antigen ◽  
Inhibition Test ◽  
Type B ◽  
Hemagglutination Inhibition Test ◽  
Ether Treatment ◽  
Type B Influenza Virus

scholarly journals DISSOCIATION OF HEMAGGLUTINATING AND ANTIBODY-MEASURING CAPACITIES OF INFLUENZA VIRUS

1947 ◽  
Vol 85 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Thomas Francis
Keyword(s):  
Influenza Virus ◽  
Specific Antibody ◽  
Serum Antibody ◽  
Allantoic Fluid ◽  
Virus Antigen ◽  
Normal Serum ◽  
Type B ◽  
Heat Stable ◽  
Labile Component ◽  
Type B Influenza Virus

Preparations of Type B influenza virus, propagated in the embryonated egg and obtained in the form of allantoic fluid, were found after heating at 56°C. for 30 minutes to retain the capacity to agglutinate erythrocytes but no longer measured specific antibody when used as antigen in titrations of serum antibody. The dissociation of the two activities suggests the presence in such virus preparations of a complex virus antigen comprising, (1) a heat-stable component which agglutinates erythrocytes and reacts primarily with specific antibody; (2) a heat-labile component reacting with a factor of normal serum which ordinarily tends to inhibit the hemagglutinating activity of influenza virus. The relation of the reagents to other known serological activities of influenza virus is being studied.

scholarly journals Measurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test

2006 ◽  
Vol 132 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
Adam Meijer ◽  
Arnold Bosman ◽  
Esther E.H.M. van de Kamp ◽  
Berry Wilbrink ◽  
Mirna Du Ry van Beest Holle ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Influenza Virus A ◽  
Hemagglutination Inhibition Test

Preparation of stable noninfective influenza virus antigens for typing by hemagglutination–inhibition

1969 ◽  
Vol 15 (2) ◽  
pp. 203-207 ◽  
Author(s):  
John R. Polley
Keyword(s):  
Influenza Virus ◽  
Gamma Irradiation ◽  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Risk Of Infection ◽  
Diagnostic Laboratory ◽  
Hemagglutination Inhibition Test ◽  
Significant Difference ◽  
Formaldehyde Treatment ◽  
Virus Antigens

For use in the hemagglutination–inhibition test, influenza virus antigens in the form of (a) untreated and inactivated allantoic fluids and (b) untreated and inactivated purified virus suspensions were compared. The inactivation procedures used were formaldehyde treatment and gamma irradiation. It was found that there was no significant difference in the potency and specificity of the purified virus antigens but that the formaldehyde-treated allantoic fluids lost potency during this process. The inactivated antigens can be lyophilized for stable storage and, after reconstitution, can be used in the diagnostic laboratory without risk of infection or interference with isolation procedures. For ease and speed of treatment, gamma irradiation is superior to treatment with formaldehyde.

Influence of albumin on rubella virus hemagglutination and the hemagglutination-inhibition test

1975 ◽  
Vol 21 (8) ◽  
pp. 1172-1177 ◽  
Author(s):  
J. B. Campbell ◽  
T. Grunberger ◽  
Mary L. Ellins
Keyword(s):  
Rubella Virus ◽  
Hemagglutination Inhibition ◽  
Virus Antigen ◽  
Inhibition Test ◽  
Human Group ◽  
Inhibitory Effects ◽  
Hemagglutination Inhibition Test ◽  
Antibody Titers ◽  
Commercial Sources

The HEPES–saline–albumin–gelatin (HSAG) diluent found optimal for agglutination of fowl erythrocytes by rubella virus antigen is also optimal for agglutination of trypsin-treated human group O cells. Albumins from different commercial sources, however, can have varying inhibitory effects on rubella hemagglutination titers. This can have a significant effect on the hemagglutination-inhibition test since antibody titers measured by this procedure are related to the amount of antigen used.

scholarly journals Serological analysis reveals circulation of influenza C viruses, Brazil

2000 ◽  
Vol 34 (2) ◽  
pp. 204-205 ◽  
Author(s):  
Fernando C Motta ◽  
Maristela O Luiz ◽  
José NSS Couceiro
Keyword(s):  
Influenza Virus ◽  
Hemagglutination Inhibition ◽  
Rio De Janeiro ◽  
Inhibition Test ◽  
Serological Analysis ◽  
Standard Methodology ◽  
Hemagglutination Inhibition Test

The circulation of influenza C viruses in Rio de Janeiro, Brazil, was studied when significant levels of antibodies were detected (56.7%) with hemagglutination inhibition test, used as a standard methodology for influenza virus studies.

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