Production of a potent complement-fixing murine leukemia virus-anti-serum from the rabbit and its reactions with various types of tissue culture cells

Virology ◽  
1968 ◽  
Vol 35 (2) ◽  
pp. 323-328 ◽  
Author(s):  
W. Schäfer ◽  
E. Seifert
Keyword(s):  
Tissue Culture ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Murine Leukemia

Induction of Immunity in Mice with Tissue Culture Cells Chronically Infected with Rauscher Murine Leukemia Virus

1968 ◽  
Vol 128 (4) ◽  
pp. 1088-1092 ◽  
Author(s):  
S. A. Mayyasi ◽  
H. F. Foster ◽  
L. M. Bulfone ◽  
B. S. Wright ◽  
G. P. Shibley
Keyword(s):  
Tissue Culture ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Murine Leukemia

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

scholarly journals THE USE OF MILLIPORE FILTERS IN ULTRASTRUCTURAL STUDIES OF CELL CULTURES AND VIRUSES

1968 ◽  
Vol 36 (1) ◽  
pp. 231-243 ◽  
Author(s):  
Robert M. McCombs ◽  
Matilda Benyesh-Melnick ◽  
J. Pierre Brunschwig
Keyword(s):  
Tissue Culture ◽  
Plasma Membranes ◽  
Fibroblast Cells ◽  
Virus Particles ◽  
Ultrastructural Studies ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Satellite Viruses ◽  
Murine Leukemia ◽  
Millipore Filters

A technique is described for embedding tissue culture cells that have been adsorbed or grown on Millipore filters. The acetone used during the embedding process rendered the filters transparent so that specific areas or cells could be chosen with the aid of the light microscope. Lymphoblastoid cells processed on the filters possessed well-defined plasma membranes and microvilli which were rarely present in cells from parallel cultures that were prepared by pelleting in the centrifuge. Fibroblast cells grown on filters retained their elongated appearance, in contrast to the rounded cells in pelleted preparations. Millipore filters were also used as a means of embedding virus pellets for sectioning. Preparations containing as few as 4 x 108 virus particles were suitable for study by the filter technique. Crude tissue-culture harvests of vaccinia virus and purified preparations of Rauscher murine leukemia and adeno-satellite viruses were successfully examined.

scholarly journals Isolation of Naturally Occurring Viruses of the Murine Leukemia Virus Group in Tissue Culture

1969 ◽  
Vol 3 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Janet W. Hartley ◽  
Wallace P. Rowe ◽  
Worth I. Capps ◽  
Robert J. Huebner
Keyword(s):  
Tissue Culture ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Group ◽  
Naturally Occurring ◽  
Murine Leukemia
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