Isolation of cowpox virus a-type inclusions and characterization of their major protein component

Virology ◽  
1986 ◽  
Vol 149 (2) ◽  
pp. 174-189 ◽  
Author(s):  
Dhavalkumar D. Patel ◽  
David J. Pickup ◽  
Wolfgang K. Joklik
Keyword(s):  
Protein Component ◽  
Major Protein ◽  
Cowpox Virus

Characterization of the Major Protein Component from Aleurone Cells of Barley (Hordeum vulgareL.)

1990 ◽  
Vol 41 (4) ◽  
pp. 385-392 ◽  
Author(s):  
T. YUPSANIS ◽  
S. R. BURGESS ◽  
P. J. JACKSON ◽  
P. R. SHEWRY
Keyword(s):  
Protein Component ◽  
Major Protein ◽  
Aleurone Cells

The Immuno-Electron Microscopic Localization of the Mo-Fe Protein Component of Nitrogenase in Cells of Azotobacter Vinelandii

1973 ◽  
Vol 31 ◽  
pp. 574-575
Author(s):  
J. T. Stasny ◽  
R. C. Burns ◽  
R. W. F. Hardy
Keyword(s):  
Cellular Localization ◽  
Direct Evidence ◽  
Azotobacter Vinelandii ◽  
Intracellular Localization ◽  
Protein Component ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Fe Protein ◽  
Intracytoplasmic Membranes

Structure-functlon studies of biological N2-fixation have correlated the presence of the enzyme nitrogenase with increased numbers of intracytoplasmic membranes in Azotobacter. However no direct evidence has been provided for the internal cellular localization of any nitrogenase. Recent advances concerned with the crystallizatiorTand the electron microscopic characterization of the Mo-Fe protein component of Azotobacter nitrogenase, prompted the use of this purified protein to obtain antibodies (Ab) to be conjugated to electron dense markers for the intracellular localization of the protein by electron microscopy. The present study describes the use of ferritin conjugated to goat antitMo-Fe protein immunoglobulin (IgG) and the observations following its topical application to thin sections of N2-grown Azotobacter.

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