Thrombin-antithrombin III complexes and fibrin degradation products following orthotopic liver transplantation

SummaryIt is still not clear whether disseminated intravascular coagulation (DIC) contributes to the hemostatic disturbances in orthotopic liver transplantation (OLT). Theoretically the lack of hepatic clearance of procoagulant factors during the anhepatic period and the release of thromboplastic material from the graft might trigger DIC. During heterotopic liver transplantation (HLT) the host liver is left in situ and procoagulant factors may still be cleared; DIC, if present, may not occur until after reperfusion. The aim of the present study was to gain more insight into the underlying mechanism of the coagulation changes during liver transplantation by comparison of OLT and HLT. Thrombin-antithrombin-III complexes (TAT), and indicator of thrombin generation, fibrin degradation products (FbDP) and routine clotting times were assayed in 12 OLTs, 18 HLTs and in a control group of 10 partial hepatic resections (PHR).TAT increased dramatically after reperfusion to 136 μg/l in OLT and to 94 μg/l in HLT (p n.s.). In contrast, FbDP levels increased only in OLT, to a maximum of 13.8 μg/ml. Routine clotting times changed mildly and similarly in both OLT and HLT.Conclusions: Graft reperfusion triggers excessive thrombin formation, but there are no other signs of subsequent DIC. Any thrombin formed is probably rapidly inhibited by antithrombin-III. The rise in FbDP during OLT is the result of increased fibrinolysis, which occurred only in OLT and not in HLT.

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Is Quantitative Determination of Fibrin(ogen) Degradation Products and Thrombin-Antithrombin III Complexes Useful to Diagnose Deep Venous Thrombosis in Outpatients?

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647113 ◽

1989 ◽

Vol 62 (04) ◽

pp. 1043-1045 ◽

Cited By ~ 12

Author(s):

Paul F M M van Bergen ◽

Eduard A R Knot ◽

Jan J C Jonker ◽

Auke C de Boer ◽

Moniek P M de Maat

Keyword(s):

Quantitative Determination ◽

Venous Thrombosis ◽

Deep Venous Thrombosis ◽

Antithrombin Iii ◽

Degradation Products ◽

Family Doctor ◽

Diagnostic Value ◽

Impedance Plethysmography ◽

Fibrin Degradation Products

SummaryWe studied the diagnostic value of recently introduced ELISA’s for the determination of thrombin-antithrombin III (TAT) complexes, fibrin degradation products (FbDP), fibrinogen degradation products (FgDP) and total degradation products (TDP) for deep venous thrombosis (DVT) in plasma of 239 consecutive outpatients, suspected for DVT by their family doctor. DVT was confirmed by impedance plethysmography in 60 patients. Using the 95th percentile range of 42 healthy volunteers the sensitivity for the detection of DVT was: 37% for TAT, 95% for TDP, 92% for FbDP and 90% for FgDP. Specificity was: 88% for TAT, 16% for TDP, 20% for FbDP and 25% for FgDP.We conclude that these assays are of little value in the diagnosis of DVT in outpatients.

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Elevation of circulating thrombin–antithrombin III complex and fibrin degradation products in urticaria: A laboratory finding unrelated to intravascular coagulopathy

The Journal of Dermatology ◽

10.1111/j.1346-8138.2008.00474.x ◽

2008 ◽

Vol 35 (5) ◽

pp. 308-310 ◽

Cited By ~ 21

Author(s):

Kimio FUJII ◽

Akiko USUKI ◽

Yuko KAN-NO ◽

Noriko OHGOU

Keyword(s):

Antithrombin Iii ◽

Degradation Products ◽

Laboratory Finding ◽

Fibrin Degradation Products ◽

Intravascular Coagulopathy ◽

Thrombin Antithrombin Iii Complex

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Thrombin antithrombin complex and IL-18 serum levels in stroke patients

Neurology International ◽

10.4081/ni.2010.e1 ◽

2010 ◽

Vol 2 (1) ◽

pp. 1 ◽

Cited By ~ 6

Author(s):

Ornella Piazza ◽

Giuliana Scarpati ◽

Simona Cotena ◽

Maria Lonardo ◽

Rosalba Tufano

Keyword(s):

Antithrombin Iii ◽

Degradation Products ◽

Focal Cerebral Ischemia ◽

Serum Levels ◽

High Serum ◽

Fibrin Degradation Products ◽

Markers Of Inflammation ◽

Complex Picture ◽

Thrombin Antithrombin Iii Complex ◽

D Dimer

The complex picture of inflammation and coagulation alterations comes to life in acute stroke phases. Increasing evidence points to a strong interaction and extensive crosstalk between the inflammation and coagulation systems: the interest towards this relationship has increased since recent experimental research showed that the early administration of antithrombin III (ATIII) decreases the volume of ischemia in mice and might be neuroprotective, playing an antiinflammatory role. We aimed to establish the extent of the relationship among markers of inflammation (S100B and IL-18) and procoagulant and fibrinolytic markers (ATIII, thrombin-antithrombin III complex (TAT), Fibrin Degradation Products (FDP), D-dimer) in 13 comatose patients affected by focal cerebral ischemia. Plasma levels of TAT, D-dimer and FDP, IL18 and S100B were increased. IL-18 and S100B high serum levels in ischemic patients suggest an early activation of the inflammatory cascade in acute ischemic injury. The basic principles of the interaction between inflammatory and coagulation systems are revised, from the perspective that simultaneous modulation of both coagulation and inflammation, rather than specific therapies aimed at one of these systems could be more successful in stroke therapy.

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Evaluation of the thromboembolic risk in hereditary quantitative and qualitative antithrombin III (at III) deficiency by measurement of plasmatic fibrin degradation products (FDF) using a monoclonal anti-D-neo antibody - role of heparan sulphates in the antithrombotic action of at III

Thrombosis Research ◽

10.1016/0049-3848(86)91450-7 ◽

1986 ◽

Vol 41 ◽

pp. 53

Keyword(s):

Antithrombin Iii ◽

Degradation Products ◽

Thromboembolic Risk ◽

Fibrin Degradation Products ◽

At Iii

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Hemostatic Indices in Healthy Foals from Birth to One Month of Age

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063879500700314 ◽

1995 ◽

Vol 7 (3) ◽

pp. 380-385 ◽

Cited By ~ 32

Author(s):

Michelle Henry Barton ◽

Debra Deem Morris ◽

Natalie Crowe ◽

Chrysann Collatos ◽

Keith W. Prasse

Keyword(s):

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Protein C ◽

Antithrombin Iii ◽

Degradation Products ◽

Plasminogen Activator Inhibitor ◽

Plasminogen Activator Inhibitor 1 ◽

Fibrin Degradation Products ◽

Tissue Plasminogen ◽

Activator Inhibitor

Hemostatic indices were determined in 45 healthy light breed foals, from birth to 1 month of age, and in 20 healthy adult (>2 years of age) light breed horses. Blood samples were obtained from each foal at 4 ages: 1) < 24 hours, 2) 4-7 days, 3) 10-14 days, and 4) 25-30 days. The following hemostatic indices were determined: platelet count; prothrombin and activated partial thromboplastin times; activity concentrations of protein C, antithrombin III, plasminogen, alpha-2 antiplasmin, tissue plasminogen activator, and plasminogen activator inhibitor- 1; plasma protein C antigen and fibrinogen concentrations; and serum fibrin degradation products concentration. Prothrombin and activated partial thromboplastin times were significantly longer at birth than in older foals. The plasma concentrations of the following were significantly lower at birth than in older foals: antithrombin III, plasminogen and tissue plasminogen activator activities, protein C antigen, and fibrinogen. Concentrations of the following were significantly higher at birth than in older foals: protein C and plasminogen activator inhibitor-1 activities and fibrin degradation products. These results indicate that hemostatic indices of neonatal foals differ significantly from those of older foals and adults. With the exceptions of antithrombin III and tissue plasminogen activator activities, all hemostatic indices measured in foals at 1 month of age were equivalent to adult values.

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