[3] Large-scale preparation of rat liver mitochondria in high yield

Author(s):  
Sidney Fleischer ◽  
J. Oliver McIntyre ◽  
Juan C. Vidal
1958 ◽  
Vol 36 (1) ◽  
pp. 1115-1119
Author(s):  
R. O. Hurst

Isolation of deoxyribonucleate from a concentrated solution of thymonucleoprotein has been achieved by disruption of the protein nucleate bond with potassium thiocyanate, adsorption of the protein on celite, and alcohol precipitation of the deoxyribonucleate from aqueous salt solution. Highly polymerized nucleate can be obtained readily from 1 lb of calf thymus tissue in high yield.


1958 ◽  
Vol 36 (11) ◽  
pp. 1115-1119 ◽  
Author(s):  
R. O. Hurst

Isolation of deoxyribonucleate from a concentrated solution of thymonucleoprotein has been achieved by disruption of the protein nucleate bond with potassium thiocyanate, adsorption of the protein on celite, and alcohol precipitation of the deoxyribonucleate from aqueous salt solution. Highly polymerized nucleate can be obtained readily from 1 lb of calf thymus tissue in high yield.


1953 ◽  
Vol 84 (3) ◽  
pp. 674-677 ◽  
Author(s):  
R. F. Witter ◽  
W. J. Pories ◽  
M. A. Cottone

2021 ◽  
Vol 16 (4) ◽  
pp. 1934578X2110076
Author(s):  
Cheng Le-qin ◽  
Ye An-qi ◽  
Zhang Hao-ran ◽  
Zhang Yue-wei ◽  
Li Ling

Ginsenoside Rg5 is a rare secondary ginsenoside with good pharmacological activity. However, preparation of Rg5 is time consuming, gives a low yield and has poor selectivity and efficiency. Therefore, the current study describes an efficient method of preparing ginsenoside Rg5 through conversion of Protopanaxadiol (PPD) type saponins in the presence of a molecular sieve and hydrochloric acid. The PPD type saponins were transformed on the surface of a molecular sieve to obtain ginsenoside Rg5 and most of the generated secondary ginsenoside was dissociates into the reaction solution, avoiding further decomposition. In addition, the optimal experimental conditions were identified to be as follows; 0.5 ml of absolute ethanol, 0.2 mol/L of hydrochloric acid, 12.5 mg of a 4A molecular sieve, a reaction time of 2.5 hours and a reaction temperature of 70 ℃. Under these experimental conditions, yields of upto 63.2% were obtained for ginsenoside Rg5. Moreover, the hydrochloric acid-catalyzed preparation of ginsenoside Rg5 effectively inhibited its decomposition in the presence of a molecular sieve. The method is therefore suitable for laboratory and industrial production with the advantages of large-scale preparation and high-yield.


1977 ◽  
Vol 80 (2) ◽  
pp. 401-408 ◽  
Author(s):  
Ernesto Bustamante ◽  
John W. Soper ◽  
Peter L. Pedersen

2019 ◽  
Vol 33 (32) ◽  
pp. 1950385
Author(s):  
Y. Z. Zhao ◽  
D. X. Du ◽  
Y. H. Wang

We reported a simple, high yield and large-scale preparation method for silver nanoparticles. Silver nanoparticles with 8–13 nm in diameter were successfully synthesized by silver nitrate as raw material, sodium borohydride as reducing agent, dispersan-5040 (polycarboxylate sodium salt) as surface modifier in aqueous solution. The effects of sodium borohydride and dispersan-5040 on the morphologies of silver nanoparticles were investigated. Further, the conductive inks were prepared with as-synthesized silver nanoparticles as conductive fillers in the presence of deionized (DI) water and additives. The effects of conductive fillers and additives on the pH, conductivity, surface tension and viscosity of the inks were investigated. The conductive patterns were fabricated by inkjet printer and their performances were studied. The sheet resistances of the printed pattern with seven-layer was 1.2 [Formula: see text] when the printed pattern was heat-treated at 120[Formula: see text]C for 60 min. We succeeded in printing a simple conductive circuit on photographic paper, which can light three 0.06 W LED beads. The successful fabrication of the functional circuit proves the feasibility of the ink and provides some ideas for future paper-based circuits.


Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.


1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.


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