[3] Micropore filter methods for leukocyte chemotaxis

Author(s):  
Peter C. Wilkinson
1975 ◽  
Vol 141 (6) ◽  
pp. 1437-1441 ◽  
Author(s):  
S R Turner ◽  
J A Campbell ◽  
W S Lynn

Polymorphonuclear leukocyte chemotaxis has been elicited by oxidized arachidonic acid and other oxidized polyenoic lipids in the Boyden micropore filter assay system. This chemotactic activity was observed in the absence of serum and chemotactic proteins. The esterfied arachidonic acid present in plasma membranes may be a precursor of chemotactic messages as well as prostaglandins in vivo.


Blood ◽  
1975 ◽  
Vol 45 (6) ◽  
pp. 863-870 ◽  
Author(s):  
JI Gallin ◽  
JA Klimerman ◽  
GA Padgett ◽  
SM Wolff

Abstract Chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic Boyden chamber technique and a new 51-Cr-labeled mononuclear radioassay with a double micropore filter system. Significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle Chediak-Higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. A similar Chediak-Higashi mononuclear leukocyte defect was noted in humans when kallikrein or dialyzable transfer factor were used as the chemotactic stimulus. Studies using smaller pore filters in the chemotactic chamber exaggerated the chemotactic defect. Serum from Chediak-Higashi subjects had normal chemotactic activity. Additional studies on the spontaneous (random) locomotion of Chediak-Higashi mononuclear leukocytes revealed normal results when a capillary tube assay system was used, but abnormal results were obtained when a Boyden chamber micropore filter assay was used, demonstrating fundamental differences in these two assays of random locomotion. It is clear from these studies that defective mononuclear leukocyte chemotaxis is another feature of the imparied host defenses in the Chediak-Higashi syndrome that may contribute to the marked susceptibility to pyogenic infections so characteristic of this dease.


Blood ◽  
1975 ◽  
Vol 45 (6) ◽  
pp. 863-870 ◽  
Author(s):  
JI Gallin ◽  
JA Klimerman ◽  
GA Padgett ◽  
SM Wolff

Chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic Boyden chamber technique and a new 51-Cr-labeled mononuclear radioassay with a double micropore filter system. Significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle Chediak-Higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. A similar Chediak-Higashi mononuclear leukocyte defect was noted in humans when kallikrein or dialyzable transfer factor were used as the chemotactic stimulus. Studies using smaller pore filters in the chemotactic chamber exaggerated the chemotactic defect. Serum from Chediak-Higashi subjects had normal chemotactic activity. Additional studies on the spontaneous (random) locomotion of Chediak-Higashi mononuclear leukocytes revealed normal results when a capillary tube assay system was used, but abnormal results were obtained when a Boyden chamber micropore filter assay was used, demonstrating fundamental differences in these two assays of random locomotion. It is clear from these studies that defective mononuclear leukocyte chemotaxis is another feature of the imparied host defenses in the Chediak-Higashi syndrome that may contribute to the marked susceptibility to pyogenic infections so characteristic of this dease.


Author(s):  
Reinhold Steinacker

AbstractTime series with a significant trend, as is now being the case for the temperature in the course of climate change, need a careful approach for statistical evaluations. Climatological means and moments are usually taken from past data which means that the statistics does not fit to actual data anymore. Therefore, we need to determine the long-term trend before comparing actual data with the actual climate. This is not an easy task, because the determination of the signal—a climatic trend—is influenced by the random scatter of observed data. Different filter methods are tested upon their quality to obtain realistic smoothed trends of observed time series. A new method is proposed, which is based on a variational principle. It outperforms other conventional methods of smoothing, especially if periodic time series are processed. This new methodology is used to test, how extreme the temperature of 2018 in Vienna actually was. It is shown that the new annual temperature record of 2018 is not too extreme, if we consider the positive trend of the last decades. Also, the daily mean temperatures of 2018 are not found to be really extreme according to the present climate. The real extreme of the temperature record of Vienna—and many other places around the world—is the strongly increased positive temperature trend over the last years.


1985 ◽  
Vol 20 (6) ◽  
pp. 553-563 ◽  
Author(s):  
L. C. Altman ◽  
R. C. Page ◽  
G. E. Vandesteen ◽  
L. I. Dixon ◽  
C. Bradford
Keyword(s):  

Chemotherapy ◽  
1986 ◽  
Vol 32 (4) ◽  
pp. 379-382 ◽  
Author(s):  
A. Eyraud ◽  
J. Descotes ◽  
J.Y. Lombard ◽  
A. Laschi-Loquerie ◽  
P. Tachon ◽  
...  

1993 ◽  
Vol 56 (4) ◽  
pp. 336-337 ◽  
Author(s):  
JOSEP SERRA BONVEHI ◽  
ROSSEND ESCOLÁ JORDÁ

The number of mesophilic aerobic colonies was determined in 72 samples of mono- and multifloral honey from various sources by the plate count and the membrane filter methods. The presence of motile colonies made the plate counts unreliable. The microorganism producing these colonies was identified as Bacillus alvei. Colony counts could only be carried out in 27 of the samples when using the plate count method, while with the membrane filter method the number of colonies was counted in all the samples.


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