[25] Rapid colony hybridization on whatman 541 paper using oligonucleotide probes

1993 ◽  
pp. 340-346 ◽  
Author(s):  
Gary V. Paddock
Keyword(s):  
Oligonucleotide Probes ◽  
Colony Hybridization

Oligonucleotide probes for the detection of TEM-1 and TEM-2 β-lactamase genes and their transposons

1987 ◽  
Vol 33 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Marc Ouellette ◽  
John J. Rossi ◽  
Renée Bazin ◽  
Paul H. Roy
Keyword(s):  
Amino Acid ◽  
Structural Gene ◽  
Restriction Fragment ◽  
Base Pair ◽  
Epidemiological Studies ◽  
Molecular Probes ◽  
Oligonucleotide Probes ◽  
Dot Blot ◽  
Colony Hybridization

We describe the use of molecular probes to detect the TEM-type β-lactamase genes. As a general probe, we prepared a 656 base pair restriction fragment, entirely within the TEM structural gene. This probe was specific for the TEM family, hybridizing only with TEM-1 and TEM-2. The TEM-1 and TEM-2 β-lactamases differ by only one amino acid. We synthesized two oligonucleotides whose central bases correspond to this difference. The use of these oligonucleotides enables us to discriminate between TEM-1 and TEM-2 genes. Using oligonucleotides homologous to parts of Tn3, we also monitored the presence of TnA-like transposons in bacteria harboring different β-lactamase genes. Only the TEM-1 and TEM-2 genes were found to be on transposons with terminal sequences identical to those of Tn3. All hybridization experiments were performed with both dot-blot and colony-hybridization techniques, and the suitability of these two methods for epidemiological studies is compared.

scholarly journals Enumeration of Legionella CFU by Colony Hybridization Using Specific DNA Probes

2002 ◽  
Vol 68 (12) ◽  
pp. 6466-6470 ◽  
Author(s):  
Wakako Satoh ◽  
Mayumi Nakata ◽  
Hiroyuki Yamamoto ◽  
Takayuki Ezaki ◽  
Keiichi Hiramatsu
Keyword(s):  
Cooling Tower ◽  
Dna Probes ◽  
Oligonucleotide Probes ◽  
Colony Hybridization

ABSTRACT Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.

Identification of Bacillus licheniformis by Colony Hybridization with 23S rRNA-targeted Oligonucleotide Probes

1994 ◽  
Vol 17 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Ralf Tatzel ◽  
Wolfgang Ludwig ◽  
Karl Heinz Schleifer ◽  
Peter R. Wallnöfer
Keyword(s):  
Bacillus Licheniformis ◽  
23S Rrna ◽  
Oligonucleotide Probes ◽  
Colony Hybridization

Identification ofBacillusstrains isolated from milk and cream with classical and nucleic acid hybridization methods

1994 ◽  
Vol 61 (4) ◽  
pp. 529-535 ◽  
Author(s):  
Ralf Tatzel ◽  
Wolfgang Ludwig ◽  
Karl Heinz Schleifer ◽  
Peter R. Wallnöfer
Keyword(s):  
Nucleic Acid Hybridization ◽  
23S Rrna ◽  
Dairy Production ◽  
Production Plant ◽  
Oligonucleotide Probes ◽  
Hybridization Method ◽  
Bacterial Strains ◽  
Identification Methods ◽  
Colony Hybridization ◽  
Physiological And Biochemical

SummaryA total of 529 bacterial strains have been isolated from milk and cream sampled at different sites in a dairy production plant under conditions selective for aerobic sporeforming bacteria. Identification with classical methods based on morphological, physiological and biochemical criteria showedBacillus licheniformisto be the most frequently occurringBacillussp. The investigation also revealed 62 unidentified strains. Classical identification methods were time consuming (3–7d), lacked specificity and —because of their dependence on phenotypic gene expression—sometimes produced ambiguous results. Consequently, a colony hybridization method developed for the identification ofB. licheniformisstrains and using non-radioactive labelled 23S rRNA targeted oligonucleotide probes was also used. Identification ofB. licheniformiswith borth classical and hybridization methods revealed diverging identification results for 70 strains.

Oligonucleotide probes for detection of cephalosporinases among Bacteroides strains.

1996 ◽  
Vol 40 (4) ◽  
pp. 1014-1016 ◽  
Author(s):  
P Mastrantonio ◽  
R Cardines ◽  
P Spigaglia
Keyword(s):  
Rapid Method ◽  
Bacteroides Fragilis ◽  
Oligonucleotide Probes ◽  
Beta Lactamase ◽  
Colony Hybridization ◽  
Bacteroides Fragilis Group

Two oligonucleotide probes selected from the sequences of cepA and cfxA genes, respectively, were used to detect beta-lactamase production among strains of the Bacteroides fragilis group. By using these probes, colony hybridization was shown to be a specific and rapid method for identifying the more prevalent beta-lactamase, CepA, and the rarer CfxA enzyme among B. fragilis strains.

Sign in / Sign up

Export Citation Format

Share Document