A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells

Cell ◽  
1976 ◽  
Vol 9 (4) ◽  
pp. 573-578 ◽  
Author(s):  
Jwu-Sheng Tung ◽  
Takashi Yoshiki ◽  
Erwin Fleissner
Blood ◽  
1969 ◽  
Vol 34 (4) ◽  
pp. 430-440 ◽  
Author(s):  
HENRY A. BATES ◽  
RUFUS O. BANKOLE ◽  
WILLIAM R. SWAIM

Abstract Plasma obtained from patients with leukemia, was concentrated by high speed centrifugation and used to prepare antisera in rabbits. Unabsorbed, anti-human leukemia plasma and anti-murine leukemia virus (Rauscher) anti-sera showed similar immunofluorescence with normal and leukemia leukocytes. After absorption of these antisera with normal human antigens, normal cells were non-reactive and leukemia cells still reactive. Each antisera was shown to contain antibodies capable of cross reacting with and/or blocking the immunofluorescent reaction of cells from acute myelogenous, stem cell, or lymphatic leukemia cases. Pre-immunization rabbit serum and anti-human normal plasma antisera failed to show these reactions. These and antisera to other antigens (human globulin, Herpesvirus, adenovirus) were also non-reactive with acute phase leukemia cells. Such findings indicated a specificity for human leukemia cells by the described antisera and that human leukemia cells and Rauscher murine leukemia virus may have common antigens. Serial studies on leukemia patients showed that the number and intensity of fluorescing cells varied with the clinical state of the patient. The three antisera gave similar reactions in acute leukemia. Differences were noted in remission. It was postulated that these differences were due to other cellular antigens, masked during the acute phase reaction. Leukocytes from patients with Hodgkin’s disease, lymphoma(s) and multiple myeloma also showed fluorescence when reacted with anti-murine leukemia. (Rauscher) virus anti-serum. Whether this common antigenicity with leukemia cells indicates similar altered cell growth and/or related etiology remains unresolved.


Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.


Author(s):  
Ray A. Weigand ◽  
Gregory C. Varjabedian

We previously described the intracellular localization of murine mammary tumor virus (MuMTV) p28 protein in thin sections (1). In that study, MuMTV containing cells fixed in 3% paraformaldehyde plus 0.05% glutaraldehyde were labelled after thin sectioning using ferritin-antiferritin in an unlabelled antibody technique. We now describe the labelling of murine leukemia virus (MuLV) particles using the unlabelled antibody technique coupled to ferritin-Fab antiferritin. Cultures of R-MuLV in NIH/3T3 cells were grown to 90% confluence (2), fixed with 2% paraformaldehyde plus 0.5% glutaraldehyde in 0.1 M cacodylate at pH 7.2, postfixed with buffered 17 OsO4, dehydrated with a series of etha-nols, and embedded in Epon. Thin sections were collected on nickel grids, incubated in 107 H2O2, rinsed in HEPES buffered saline, and subjected to the immunoferritin labelling procedure. The procedure included preincubation in 27 egg albumin, a four hour incubation in goat antisera against purified gp69/71 of MuLV (3) (primary antibody), incubation in F(ab’)2 fragments of rabbit antisera to goat IgG (secondary antibody), incubation in apoferritin, incubation in ferritin-Fab ferritin, and a brief fixation with 2% glutaraldehyde. The sections were stained with uranyl acetate and examined in a Siemens IA electron microscope at an accelerating voltage of 60 KV.


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