Control of metallothionein expression by hormones and stressors in cultured fish cell lines

1993 ◽  
Vol 35 (1-2) ◽  
pp. 25-28 ◽  
Author(s):  
David Burgess ◽  
Nick Frerichs ◽  
Stephen George
Keyword(s):  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Cultured Fish

Cytotoxicity evaluation of chemicals using cultured fish cells

2000 ◽  
Vol 42 (7-8) ◽  
pp. 277-282 ◽  
Author(s):  
M. Mori ◽  
M. Wakabayashi
Keyword(s):  
Cell Lines ◽  
Copper Chloride ◽  
Nickel Chloride ◽  
Neutral Red ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Neutral Red Assay ◽  
Cultured Fish ◽  
Fish Cells ◽  
Very High

Development of simple and rapid cytotoxicity assays using fish cell lines was attempted. Cytotoxicity of chemicals was evaluated by neutral red assay using five monolayer-cultured fish cell lines, BB cells, CHSE-214 cells, EPC cells, FHM cells, RTG-2 cells, and one suspension-cultured fish cell line, CHSE-sp cells. The strength of cytotoxicity was observed in an order of cadmium chloride>zinc sulfate>copper chloride>nickel chloride. The concentration of no observable effect on the CHSE-214 cells viability was 2%(v/v) for DMSO and 0.5%(v/v) for DMSO containing 20 mg · ml–1 taurodeoxycholic acid. The correlation between the 24-hour NR50 values of eleven chemicals to the CHSE-sp cells and those to the CHSE-214 cells was very high (r=0.98). The cytotoxicity assay using suspension-cultured fish cells was found to be one of valuable device for screening the toxicity of chemicals for fish.

scholarly journals Isolation, Identification, and Genomic Analysis of a Novel Reovirus from Healthy Grass Carp and Its Dynamic Proliferation In Vitro and In Vivo

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 690
Author(s):  
Ke Zhang ◽  
Wenzhi Liu ◽  
Yiqun Li ◽  
Yong Zhou ◽  
Yan Meng ◽  
...  
Keyword(s):  
Cell Lines ◽  
Grass Carp ◽  
Amino Acid Sequences ◽  
Gibel Carp ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Total Size ◽  
Sequence Comparisons ◽  
Grass Carp Reovirus

A new grass carp reovirus (GCRV), healthy grass carp reovirus (HGCRV), was isolated from grass carp in 2019. Its complete genome sequence was determined and contained 11 dsRNAs with a total size of 23,688 bp and 57.2 mol% G+C content, encoding 12 proteins. All segments had conserved 5' and 3' termini. Sequence comparisons showed that HGCRV was closely related to GCRV-873 (GCRV-I; 69.57–96.71% protein sequence identity) but shared only 22.65–45.85% and 23.37–43.39% identities with GCRV-HZ08 and Hubei grass carp disease reovirus (HGDRV), respectively. RNA-dependent RNA-polymerase (RdRp) protein-based phylogenetic analysis showed that HGCRV clustered with Aquareovirus-C (AqRV-C) prior to joining a branch common with other aquareoviruses. Further analysis using VP6 amino acid sequences from Chinese GCRV strains showed that HGCRV was in the same evolutionary cluster as GCRV-I. Thus, HGCRV could be a new GCRV isolate of GCRV-I but is distantly related to other known GCRVs. Grass carp infected with HGCRV did not exhibit signs of hemorrhage. Interestingly, the isolate induced a typical cytopathic effect in fish cell lines, such as infected cell shrank, apoptosis, and plague-like syncytia. Further analysis showed that HGCRV could proliferate in grass carp liver (L28824), gibel carp brain (GiCB), and other fish cell lines, reaching a titer of up to 7.5 × 104 copies/μL.

Development, characterization, conservation and storage of fish cell lines: a review

2010 ◽  
Vol 37 (1) ◽  
pp. 1-20 ◽  
Author(s):  
W. S. Lakra ◽  
T. Raja Swaminathan ◽  
K. P. Joy
Keyword(s):  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
And Storage

Erratum to “In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines” [Toxicology in Vitro 18 (2004) 365–376]

2004 ◽  
Vol 18 (5) ◽  
pp. 731
Author(s):  
S Nı́ Shúilleabháin ◽  
C Mothersill ◽  
D Sheehan ◽  
N.M O'Brien ◽  
J O'Halloran ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Metal Salts ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Zinc Metal ◽  
Cytotoxicity Testing

scholarly journals The interaction mechanism of marine birnavirus (MABV) in fish cell lines.

Uirusu ◽  
2005 ◽  
Vol 55 (1) ◽  
pp. 133-144 ◽  
Author(s):  
Syunichirou OSHIMA ◽  
Masayuki IMAJOH ◽  
Takeshi HIRAYAMA
Keyword(s):  
Cell Lines ◽  
Interaction Mechanism ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Marine Birnavirus

scholarly journals Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 75 ◽  
Author(s):  
Sebastian Escobar-Aguirre ◽  
Duxan Arancibia ◽  
Amanda Escorza ◽  
Cristián Bravo ◽  
María Andrés ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cell Lines ◽  
Gene Function ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Guide Rna ◽  
Bicistronic Vector ◽  
Expression Platform ◽  
Fish Cells ◽  
U6 Rna

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters to drive the expression of both small guide RNA (sgRNA) and the S. pyogenes Cas9 (spCas9) protein within a single expression platform. Until now, fish cells have been modified using co-transfection of the mRNA of both the sgRNA and the spCas9. In the present study, we describe the optimization of a new vector for the expression of a CRISPR/Cas9 system, designed to edit the genome of fish cell lines, that combines a gene reporter (mCherry), sgRNA, and spCas9 in a single vector, facilitating the study of the efficiency of piscine and non-piscine promoters. A cassette containing the zebrafish U6 RNA III polymerase (U6ZF) promoter was used for the expression of the sgRNA. The new plasmid displayed the expression of spCas9, mCherry, and sgRNA in CHSE/F fish cells. The results demonstrate the functionality of the mammalian promoter and the U6ZF promoter in fish cell lines. This is the first approach aimed at developing a unified genome editing system in fish cells using bicistronic vectors, thus creating a powerful biotechnological platform to study gene function.

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