The micronucleus test as part of a short-term mutagenicity test program for the prediction of carcinogenicity evaluated by 143 agents tested

1980 ◽  
Vol 75 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Dag Jenssen ◽  
Claes Ramel
Keyword(s):  
Micronucleus Test ◽  
Test Program ◽  
Short Term ◽  
Mutagenicity Test

scholarly journals Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo

2014 ◽  
Vol 50 (2) ◽  
pp. 251-256
Author(s):  
Igor Vivian de Almeida ◽  
Giovana Domingues ◽  
Lilian Capelari Soares ◽  
Elisângela Düsman ◽  
Veronica Elisa Pimenta Vicentini
Keyword(s):  
Rattus Norvegicus ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Term Treatment ◽  
Genotoxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
Chromosomal Aberration Test

Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic. However, considering the adverse effects of using this benzodiazepine, more studies are required.

scholarly journals Antitumor Organometallics. IV. The Mutagenic Potential of Some Diphenylantimony(III) Dithiophosphorus Derivatives

1994 ◽  
Vol 1 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Carmen Socaciu ◽  
Ioan Pasca ◽  
Cristian Silvestru ◽  
Adela Bara ◽  
Ionel Haiduc
Keyword(s):  
Cytogenetic Analysis ◽  
Micronucleus Test ◽  
Ascites Tumor ◽  
Short Term ◽  
Mutagenic Potential ◽  
Tumor Bearing ◽  
Ehrlich Ascites ◽  
Antitumor Properties ◽  
Mutagenic Effects ◽  
Derivatives Of

Two diphenylantimony(III) derivatives of dithiophosphorus ligands, i.e. Ph2SbS2PPh2 and Ph2SbS2P(OPr-i)2, which were previously found to exhibit antitumor properties, have been now investigated for potential mutagenic effects in healthy and Ehrlich ascites tumor-bearing mice. Two short-term tests, i.e. the micronucleus test and the cytogenetic analysis, were used as end-points for mutagenicity. The results are consistent with a mutagenic potential for both organoantimony(III) compounds tested, the effect being higher for the phosphorodithioato derivative.

scholarly journals CL 64, 855, a potent do anti-Trypanosoma cruzi drug, is also mutagenic in the salmonella/microsome assay

1986 ◽  
Vol 81 (1) ◽  
pp. 49-52 ◽  
Author(s):  
R. C. C. Ferreira ◽  
L. C. S. Ferreira
Keyword(s):  
Trypanosoma Cruzi ◽  
Salmonella Typhimurium ◽  
Rat Liver ◽  
Metabolic Activation ◽  
Mutagenic Action ◽  
Short Term ◽  
Mutagenicity Test

The nitroimidazole-tiadiazole derivative CL 64,855 (2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole, a potent anti-trypanosomal drug, was assayed in a short-term bacterial mutagenicity test with Salmonella typhimurium strains TA 98, TA 100 and TA 102. Results indicate that CL 64,855 is a potent frameshift mutagen detected by strains TA 98 and TA 102. CL 64,855 was able to revert the indicators strains at concentrations as low as 0.1 µg/plate. Metabolic activation experiments with rat liver microsomal fractions did not increase the mutagenic action of Cl 64,855.

Application of micronucleus in vitro assay to micropollutants in river water

1997 ◽  
Vol 35 (8) ◽  
pp. 9-13 ◽  
Author(s):  
Youn-Son Chung ◽  
Kazuhiro Ichikawa ◽  
Hideo Utsumi
Keyword(s):  
River Water ◽  
Mammalian Cells ◽  
Micronucleus Test ◽  
In Vitro Test ◽  
Vitro Assay ◽  
Mutagenicity Test ◽  
Vitro Test ◽  
Kanagawa Prefecture ◽  
The Difference

To determine the genotoxicity of river water towards mammalian cells, we applied Micronucleus in vitro test using mammalian cells to the samples taken from river Tamagawa located between Tokyo and Kanagawa prefecture. Water samples were condensed by Sep-pak cartridges and extracted by dichloromethane and methanol. Positive genotoxicity was observed in methanol extracts from sampling stations of Hinobashi and Marukobashi, while no dichloromethane extracts showed genotoxicity, suggesting that polar genotoxic micropollutants may be contained in the water of Tamagawa, at least in its down-stream. Significantly high mutagenicity also detected from Hinobashi and Marukobashi by Ames mutagenicity test using Salmonella Typhimurium, and some difference was obtained in sensitivity between the two methods. This may arise from the difference in species used, that is, mammalian cells in micronucleus test and bacteria in Ames mutagenicity test.

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