Kappa light chain predominance in serum and cerebrospinal fluid from human immunodeficiency virus type 1 (HIV-1)-infected patients

1991 ◽  
Vol 32 (3) ◽  
pp. 259-268 ◽  
Author(s):  
Luigi M.E. Grimaldi ◽  
Antonella Castagna ◽  
Davide Maimone ◽  
Gian Vito Martino ◽  
Alberto Dolci ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cerebrospinal Fluid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Light Chain ◽  
Kappa Light Chain ◽  
Immunodeficiency Virus ◽  
Hiv 1

scholarly journals Comparison of Human Immunodeficiency Virus Type 1 RNA Sequence Heterogeneity in Cerebrospinal Fluid and Plasma

2000 ◽  
Vol 38 (12) ◽  
pp. 4637-4639 ◽  
Author(s):  
Yi-Wei Tang ◽  
Joe T.-J. Huong ◽  
Robert M. Lloyd ◽  
Paul Spearman ◽  
David W. Haas
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cerebrospinal Fluid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Plasma Samples ◽  
Sequence Heterogeneity ◽  
Immunodeficiency Virus ◽  
Viral Sequences ◽  
Hiv 1

The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two commercial genotyping methodologies. In individual subjects, the sequences in CSF samples differed significantly from those in plasma. In contrast, the viral sequences in CSF at the baseline did not differ from the sequences in CSF during treatment. Similarly, viral sequences in plasma did not vary over this interval. This study provides evidence that HIV-1 RNA in CSF and plasma arise from distinct compartments.

scholarly journals The clinical significance of human immunodeficiency virus type 1- associated paraproteins

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2471-2475 ◽  
Author(s):  
VL Ng ◽  
KH Chen ◽  
KM Hwang ◽  
H Khayam-Bashi ◽  
MS McGrath
Keyword(s):  
Human Immunodeficiency Virus ◽  
Clinical Significance ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Light Chain ◽  
Viral Antigens ◽  
Sds Page ◽  
Immunodeficiency Virus ◽  
Hiv 1

Abstract We observed and characterized paraproteins present in the serum of seven human immunodeficiency virus type 1 (HIV-1)-infected individuals. Immunoglobulin (Ig) subclass typing performed on these paraproteins identified five as IgG1 kappa, one as an IgG3 lambda, and one as an IgA lambda. The IgG1 kappa paraproteins, purified by high-pressure liquid chromatography, contained the majority of anti-HIV-1 antibody reactivity present in the five serum specimens (ranging from 1:5,000 to 1:500,000) as demonstrated by immunoblot. All five IgG1 paraproteins had at least two light chain species as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the antibodies were reactive with multiple HIV-1 viral antigens. In contrast, the electrophoretically purified IgG3 lambda and IgA lambda paraproteins did not react with HIV-1 antigens and only one light chain species was detected by SDS-PAGE. The subsequent clinical evaluation of these patients following the initial observation of paraproteinemias failed to correlate the presence of paraproteins with the development of lymphoma over a 2 to 3 year period. These data support the hypothesis that IgG1 paraproteins present in the sera of HIV-1 infected individuals reflect a normal albeit exuberant polyclonal immune response to HIV-1 viral antigens. In contrast, the clinical significance of an IgG3 lambda or an IgA lambda paraprotein is unclear at present.

Cerebrospinal fluid human immunodeficiency virus type 1 (HIV-1) p24 antigen levels in HIV-1-related dementia

1994 ◽  
Vol 36 (1) ◽  
pp. 32-39 ◽  
Author(s):  
W. Royal ◽  
O. A. Selnes ◽  
M. Concha ◽  
T. E. Nance-Sproson ◽  
J. C. McArthur
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cerebrospinal Fluid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunodeficiency Virus ◽  
P24 Antigen ◽  
Hiv 1

scholarly journals The clinical significance of human immunodeficiency virus type 1- associated paraproteins

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2471-2475
Author(s):  
VL Ng ◽  
KH Chen ◽  
KM Hwang ◽  
H Khayam-Bashi ◽  
MS McGrath
Keyword(s):  
Human Immunodeficiency Virus ◽  
Clinical Significance ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Light Chain ◽  
Viral Antigens ◽  
Sds Page ◽  
Immunodeficiency Virus ◽  
Hiv 1

We observed and characterized paraproteins present in the serum of seven human immunodeficiency virus type 1 (HIV-1)-infected individuals. Immunoglobulin (Ig) subclass typing performed on these paraproteins identified five as IgG1 kappa, one as an IgG3 lambda, and one as an IgA lambda. The IgG1 kappa paraproteins, purified by high-pressure liquid chromatography, contained the majority of anti-HIV-1 antibody reactivity present in the five serum specimens (ranging from 1:5,000 to 1:500,000) as demonstrated by immunoblot. All five IgG1 paraproteins had at least two light chain species as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the antibodies were reactive with multiple HIV-1 viral antigens. In contrast, the electrophoretically purified IgG3 lambda and IgA lambda paraproteins did not react with HIV-1 antigens and only one light chain species was detected by SDS-PAGE. The subsequent clinical evaluation of these patients following the initial observation of paraproteinemias failed to correlate the presence of paraproteins with the development of lymphoma over a 2 to 3 year period. These data support the hypothesis that IgG1 paraproteins present in the sera of HIV-1 infected individuals reflect a normal albeit exuberant polyclonal immune response to HIV-1 viral antigens. In contrast, the clinical significance of an IgG3 lambda or an IgA lambda paraprotein is unclear at present.

scholarly journals Detection of Human Cytomegalovirus pp67 Late Gene Transcripts in Cerebrospinal Fluid of Human Immunodeficiency Virus Type 1-Infected Patients by Nucleic Acid Sequence-Based Amplification

2000 ◽  
Vol 38 (5) ◽  
pp. 1920-1925 ◽  
Author(s):  
Fan Zhang ◽  
Surya Tetali ◽  
Xue Ping Wang ◽  
Mark H. Kaplan ◽  
Frans V. Cromme ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cerebrospinal Fluid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Human Cytomegalovirus ◽  
Cns Disease ◽  
Immunodeficiency Virus ◽  
Dna Pcr ◽  
Hiv 1

This study examined the clinical correlation between the presence of human cytomegalovirus (HCMV) pp67 mRNA in cerebrospinal fluid (CSF) and active HCMV central nervous system (CNS) disease in patients with human immunodeficiency virus type 1 (HIV-1). In total, 76 CSF specimens collected from 65 HIV-1-positive patients diagnosed with HCMV CNS disease, other non-HCMV-related CNS diseases, or no CNS disease were tested for the presence of HCMV pp67 mRNA using the NucliSens cytomegalovirus (CMV) pp67 assay (Organon Teknika, Durham, N.C.). The results were compared to those of a nested PCR for the detection of HCMV glycoprotein B DNA and to those obtained by viral culture (54 samples). CSF specimens collected from patients without HCMV CNS disease yielded the following results: pp67 assay negative, 62 of 62 specimens; culture negative, 41 of 41 specimens; and PCR negative, 56 of 62 specimens (6 specimens were positive). CSF specimens collected from patients with HCMV CNS disease yielded the following results: pp67 assay positive, 9 of 13 specimens; PCR positive, 13 of 13 specimens; and culture positive, 2 of 13 specimens. After resolution of the discordant results, the following positive and negative predictive values (PPV and NPV, respectively) for the diagnosis of HCMV CNS disease were determined. The PPV for PCR, pp67 assay, and culture were 68.4, 100, and 100%, respectively, and the NPV for PCR, pp67 assay, and culture were 100, 97.0, and 82.7%, respectively. The sensitivities for DNA PCR, pp67 assay, and culture for the detection of HCMV were 100, 84.6, and 18%, respectively, and the clinical specificities were 90.5, 100, and 100%, respectively. This study indicates that the detection of HCMV pp67 mRNA in CSF has good correlation with active HCMV CNS disease, whereas CSF culture is insensitive and qualitative DNA PCR may detect latent nonreplicating virus in CSF from patients without HCMV CNS disease.

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