Evaluation of potential mutagenic effect of the liquid smoke preparation UTP in vivo: cytogenetic analysis of mouse bone marrow

1994 ◽  
Vol 323 (1-2) ◽  
pp. 89-92 ◽  
Author(s):  
Alena Kažimírová ◽  
Angelika Jablonická
Keyword(s):  
Bone Marrow ◽  
Cytogenetic Analysis ◽  
Mutagenic Effect ◽  
Mouse Bone Marrow ◽  
Liquid Smoke

scholarly journals Assessment of mutagenicity and cytotoxicity of Solanum paniculatum L. extracts using in vivo micronucleus test in mice

2010 ◽  
Vol 70 (3) ◽  
pp. 601-606 ◽  
Author(s):  
PM. Vieira ◽  
SC. Santos ◽  
L. Chen-Chen
Keyword(s):  
Bone Marrow ◽  
Micronucleus Test ◽  
Folk Medicine ◽  
Mutagenic Effect ◽  
Mouse Bone Marrow ◽  
Cytotoxic Effects ◽  
Tropical America ◽  
Higher Doses ◽  
Ethanolic Leaf Extract

Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the Brazilian Savanna region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and gastric dysfunctions, as well as hangovers. Because of the wide use of this plant as a therapeutic resource and food, the present study aimed at evaluating the mutagenic and cytotoxic effects of S. paniculatum ethanolic leaf and fruit extracts using the mouse bone marrow micronucleus test. Our results indicate that neither S. paniculatum ethanolic leaf extract nor its ethanolic fruit extract exhibited mutagenic effect in mice bone marrow; however, at higher doses, both extracts presented cytotoxic activity.

The mutagenic effect of trifluralin-containing herbicide on mouse bone marrow in vivo

1979 ◽  
Vol 3 (4) ◽  
pp. 454-457 ◽  
Author(s):  
M. Nehéz ◽  
A. Páldy ◽  
A. Selypes ◽  
M. Körösfalvi ◽  
I. Lörinczi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mutagenic Effect ◽  
Mouse Bone Marrow

Expression of human adenosine deaminase in murine hematopoietic cells

1988 ◽  
Vol 8 (12) ◽  
pp. 5116-5125
Author(s):  
J W Belmont ◽  
G R MacGregor ◽  
K Wager-Smith ◽  
F A Fletcher ◽  
K A Moore ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adenosine Deaminase ◽  
High Titer ◽  
Virus Production ◽  
Marrow Transplant ◽  
Mouse Bone Marrow ◽  
Regulation Of Expression ◽  
Murine Bone Marrow

Multiple replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model. High-titer virus production was obtained from vectors by using both a retrovirus long terminal repeat promoter and internal transcriptional units with human c-fos and herpes virus thymidine kinase promoters. After infection of primary murine bone marrow with one of these vectors, human adenosine deaminase was detected in 60 to 85% of spleen colony-forming units and in the blood of 14 of 14 syngeneic marrow transplant recipients. This system offers the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.

scholarly journals Early megakaryocyte lineage-committed progenitors in adult mouse bone marrow

2021 ◽  
Author(s):  
Zixian Liu ◽  
Jinhong Wang ◽  
Miner Xie ◽  
Peng Wu ◽  
Yao Ma ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Single Cell ◽  
Pcr Analysis ◽  
Mouse Bone Marrow ◽  
Hematopoietic Stem ◽  
Colony Assay ◽  
Megakaryocyte Progenitors ◽  
Cell Colony

Hematopoietic stem cells (HSCs) have been considered to progressively lose their self-renewal and differentiation potentials prior to the commitment to each blood lineage. However, recent studies have suggested that megakaryocyte progenitors are generated at the level of HSCs. In this study, we newly identified early megakaryocyte lineage-committed progenitors (MgPs) in CD201-CD48- cells and CD48+ cells separated from the CD150+CD34-Kit+Sca-1+Lin- HSC population of the bone marrow in C57BL/6 mice. Single-cell transplantation and single-cell colony assay showed that MgPs, unlike platelet-biased HSCs, had little repopulating potential in vivo, but formed larger megakaryocyte colonies in vitro (on average eight megakaryocytes per colony) than did previously reported megakaryocyte progenitors (MkPs). Single-cell RNA-sequencing supported that these MgPs lie between HSCs and MkPs along the megakaryocyte differentiation pathway. Single-cell colony assay and single-cell RT-PCR analysis suggested the coexpression of CD41 and Pf4 is associated with megakaryocyte colony-forming activity. Single-cell colony assay of a small number of cells generated from single HSCs in culture suggested that MgPs are not direct progeny of HSCs. In this study, we propose a differentiation model in which HSCs give rise to MkPs through MgPs.

scholarly journals O 6-Benzylguanine Potentiates the In Vivo Toxicity and Clastogenicity of Temozolomide and BCNU in Mouse Bone Marrow

Blood ◽  
1997 ◽  
Vol 89 (5) ◽  
pp. 1566-1573 ◽  
Author(s):  
Nachimuthu Chinnasamy ◽  
Joseph A. Rafferty ◽  
Ian Hickson ◽  
John Ashby ◽  
Helen Tinwell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Antitumor Agents ◽  
Alkylating Agents ◽  
Micronucleus Assay ◽  
Hematological Toxicity ◽  
Mouse Bone Marrow ◽  
Macrophage Colony ◽  
Lower Magnitude ◽  
Marrow Cellularity

Abstract The effects of treatment of mice with O6-benzylguanine (O6-BeG) on the levels of O6-alkylguanine-DNA alkyltransferase (ATase) in the hematopoietic compartment and on the in vivo sensitivity of hematopoietic progenitor cells to the toxic and clastogenic effects of the antitumor agents 1,3-bis(2-chloroethyl)-nitrosourea (BCNU) and temozolomide were studied. When the overall effects of BCNU alone or with O6-BeG pretreatment were compared, dose potentiating factors of 4.17 for marrow cellularity, 4.57 for granulocyte macrophage-colony forming cells (GM-CFC) and 8.25 for colony forming unit-spleen (CFU-S) in O6-BeG pretreated versus nonpretreated animals were observed. A similar trend of dose potentiation was observed for temozolomide, although it was of lower magnitude: 1.20 for marrow cellularity, 1.63 for GM-CFC, and 1.68 for CFU-S. When the clastogenic effects of BCNU and temozolomide were examined in the mouse bone marrow micronucleus assay, a significantly (P < .05 to .001) higher frequency of micronuclei formation was observed in mice that received O6-BeG pretreatment compared with mice that received no pretreatment. These data suggest that the use of O6-BeG as a tumor-sensitizing agent before treatment of patients with O6-alkylating agents may lead to more severe hematological toxicity and possibly to an increased incidence of secondary leukemias as a result of elevated mutation frequencies in these patients.

scholarly journals Two classes of murine granulocyte progenitor cells expressed in plasma clot diffusion chamber cultures

Blood ◽  
1982 ◽  
Vol 59 (4) ◽  
pp. 838-843 ◽  
Author(s):  
HN Steinberg ◽  
PL Page ◽  
SH Robinson
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Cytosine Arabinoside ◽  
Diffusion Chamber ◽  
Velocity Sedimentation ◽  
Mouse Bone Marrow ◽  
Plasma Clot ◽  
Residual Concentration

Abstract Two distinct classes of granulocyte progenitor cells present in normal mouse bone marrow are expressed sequentially in the vivo plasma clot diffusion chamber culture system. By several criteria, progenitor cells giving rise to granulocyte colonies on day 4 of culture (CFU-D4) are different from those giving rise to colonies on day 7 (CFU-D7). These differences include: cell cycle activity as measured by in vitro incubation with cytosine arabinoside, residual concentration in the bone marrow after in vivo treatment of donor mice with cytosine arabinoside or methotrexate, resistance to osmotic lysis, size as determined by velocity sedimentation, and the morphology of the granulocyte colonies to which these cells give rise. The CFU-D7 appears to represent an earlier progenitor cell than the CFU-D4 in the differentiation pathway of the granulocyte and is analagous in many respects to the BFU-E in the erythroid pathway.

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