scholarly journals Assessment of mutagenicity and cytotoxicity of Solanum paniculatum L. extracts using in vivo micronucleus test in mice

2010 ◽  
Vol 70 (3) ◽  
pp. 601-606 ◽  
Author(s):  
PM. Vieira ◽  
SC. Santos ◽  
L. Chen-Chen
Keyword(s):  
Bone Marrow ◽  
Micronucleus Test ◽  
Folk Medicine ◽  
Mutagenic Effect ◽  
Mouse Bone Marrow ◽  
Cytotoxic Effects ◽  
Tropical America ◽  
Higher Doses ◽  
Ethanolic Leaf Extract

Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the Brazilian Savanna region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and gastric dysfunctions, as well as hangovers. Because of the wide use of this plant as a therapeutic resource and food, the present study aimed at evaluating the mutagenic and cytotoxic effects of S. paniculatum ethanolic leaf and fruit extracts using the mouse bone marrow micronucleus test. Our results indicate that neither S. paniculatum ethanolic leaf extract nor its ethanolic fruit extract exhibited mutagenic effect in mice bone marrow; however, at higher doses, both extracts presented cytotoxic activity.

scholarly journals Assessment of the mutagenicity of the technical product of N-(1-ethylpropyl)-2,6-dinitro -3,4-xylidinen

2020 ◽  
Vol 99 (4) ◽  
pp. 418-424
Author(s):  
О. В. Егорова ◽  
Наталия Алексеевна Илюшина ◽  
Н. С. Аверьянова ◽  
Г. В. Масальцев ◽  
О. О. Дмитричева
Keyword(s):  
Bone Marrow ◽  
Micronucleus Test ◽  
Fold Increase ◽  
Negative Control ◽  
Mouse Bone Marrow ◽  
Technical Product ◽  
Mutagenic Property ◽  
Mutagenic Effects ◽  
Technical Products

Introduction. Evaluation of genotoxicity of the pesticide technical products is one of the mandatory requirements for their toxicological and hygienic assessment. The data about mutagenic property is ambiguous for some pesticides. This may be due to the use of various active ingredients of technical products of the pesticide for testing, as they may have different profiles of relevant impurities, some of which may be potentially genotoxic. Material and methods. A technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine was tested using the bacterial reverse mutation method with Salmonella typhimurium (Ames test) and the in vivo mammalian micronucleus analysis in mouse bone marrow erythrocytes. Results. Statistically significant dose-dependent mutagenic effects of the technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine were revealed for TA97 (+S9 / -S9); TA100 (+S9 / -S9); TA102 (+S9 / -S9) and TA98 (+S9 / -S9) strains. In all cases, the fold increase of the revertant numbers mediated by the tested substance compared with the concurrent negative control was > 2 except TA98 in the presence of S9. In the micronucleus test, the technical product did not induce a statistically significant increase in the frequency of the micronucleated polychromatophilic erythrocytes in CD-1 mouse bone marrow up to 2000 mg/kg bw. Conclusion. The data suggest all technical products of pesticides entering the market should be tested for the potential genotoxicity. In such a case it is necessary to use at least two methods on different test systems for obtaining reliable results.

scholarly journals Chemoprotective Effects of Broccoli Juice Treated with High Pressure

2011 ◽  
Vol 24 (No. 1) ◽  
pp. 19-25 ◽  
Author(s):  
L. MANDELOVÁ ◽  
J. TOTUŠEK
Keyword(s):  
Bone Marrow ◽  
High Pressure ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Polyphenolic Compounds ◽  
Mouse Bone Marrow ◽  
Single Administration ◽  
Pressure Method ◽  
Mouse Bone Marrow Cells

We investigated chemoprotective effects of broccoli juice, treated with high pressure method, using 500 MPa for a period of 10 minutes. By the use of this method, the conservation of nutritionally important substances occurs (for example vitamins, polyphenolic compounds, glucosinolates and other constituent substances). We followed the inhibition of mutagenicity of the model mutagen, N-nitroso-N-methylurea (MNU), by means of in vivo micronucleus test. After fourteen-day application of broccoli juice (0.2 ml/10 g of body weight of mouse) and after a single administration of MNU mutagen (50 mg/kg), a statistically significant decrease (p < 0.01) occurred of the number of micronuclei induced by the application of MNU. Broccoli juice treated with high pressure therefore seems to have a preventive potential against MNU-induced micronuclei formation in BALB/C mouse bone marrow cells.

Assessment of the mutagenicity of the technical product of N-(1-ethylpropyl)-2,6-dinitro -3,4-xylidinen

2020 ◽  
Vol 99 (4) ◽  
pp. 418-424
Author(s):  
O. V. Egorova ◽  
Nataliya A. Ilyushina ◽  
N. S. Averianova ◽  
G. V. Masaltsev ◽  
O. O. Dmitricheva
Keyword(s):  
Bone Marrow ◽  
Micronucleus Test ◽  
Fold Increase ◽  
Negative Control ◽  
Mouse Bone Marrow ◽  
Technical Product ◽  
Mutagenic Property ◽  
Mutagenic Effects ◽  
Technical Products

Introduction. Evaluation of genotoxicity of the pesticide technical products is one of the mandatory requirements for their toxicological and hygienic assessment. The data about mutagenic property is ambiguous for some pesticides. This may be due to the use of various active ingredients of technical products of the pesticide for testing, as they may have different profiles of relevant impurities, some of which may be potentially genotoxic. Material and methods. A technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine was tested using the bacterial reverse mutation method with Salmonella typhimurium (Ames test) and the in vivo mammalian micronucleus analysis in mouse bone marrow erythrocytes. Results. Statistically significant dose-dependent mutagenic effects of the technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine were revealed for TA97 (+S9 / -S9); TA100 (+S9 / -S9); TA102 (+S9 / -S9) and TA98 (+S9 / -S9) strains. In all cases, the fold increase of the revertant numbers mediated by the tested substance compared with the concurrent negative control was > 2 except TA98 in the presence of S9. In the micronucleus test, the technical product did not induce a statistically significant increase in the frequency of the micronucleated polychromatophilic erythrocytes in CD-1 mouse bone marrow up to 2000 mg/kg bw. Conclusion. The data suggest all technical products of pesticides entering the market should be tested for the potential genotoxicity. In such a case it is necessary to use at least two methods on different test systems for obtaining reliable results.

In vivo genotoxicity of selected herbicides in the mouse bone-marrow micronucleus test

1997 ◽  
Vol 71 (3) ◽  
pp. 193-197 ◽  
Author(s):  
T. Gebel ◽  
S. Kevekordes ◽  
K. Pav ◽  
R. Edenharder ◽  
H. Dunkelberg
Keyword(s):  
Bone Marrow ◽  
Micronucleus Test ◽  
Mouse Bone Marrow ◽  
Bone Marrow Micronucleus

Toxicology of Diethylene Glycol Butyl Ether 3. Genotoxicity Evaluation in an In Vitro Gene Mutation Assay and an In Vivo Cytogenetic Test

1993 ◽  
Vol 12 (2) ◽  
pp. 155-159 ◽  
Author(s):  
B. Bhaskar Gollapudi ◽  
V. A. Linscombe ◽  
M. L. Mcclintock ◽  
A. K. Sinha ◽  
C. R. Stack
Keyword(s):  
Bone Marrow ◽  
Gene Mutation ◽  
Micronucleus Test ◽  
Mouse Bone Marrow ◽  
Butyl Ether ◽  
Polychromatic Erythrocytes ◽  
Mutation Assay ◽  
Gene Mutation Assay

DGBE was evaluated in a forward gene mutation assay at the HGPRT locus of CHO cells in culture and in an in vivo mouse bone marrow micronucleus test for cytogenetic damage. DGBE did not elicit a positive response in the CHO/HGPRT assay when tested up to a maximum concentration of 5000 μg/ml with and without an external metabolic activation system (S-9). In the micronucleus test employing three post-treatment bone marrow sampling times (24, 48, and 72 hr), DGBE was ineffective in increasing the incidence of micronucleated polychromatic erythrocytes (MN-PCE) when tested in both sexes up to a maximum tolerated dose of 3300 mg/kg body weight. Thus, these data and those of others indicate a general lack of genotoxic potential for DGBE in short-term tests.

The mutagenic effect of trifluralin-containing herbicide on mouse bone marrow in vivo

1979 ◽  
Vol 3 (4) ◽  
pp. 454-457 ◽  
Author(s):  
M. Nehéz ◽  
A. Páldy ◽  
A. Selypes ◽  
M. Körösfalvi ◽  
I. Lörinczi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mutagenic Effect ◽  
Mouse Bone Marrow
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