Determination of vaccine-induced and naturally acquired class-specific mumps antibodies by two indirect enzyme-linked immunosorbent assays

1986 ◽  
Vol 13 (2) ◽  
pp. 91-106 ◽  
Author(s):  
Giovanni Nigro ◽  
Fulvio Nanni ◽  
Mario Midulla
Keyword(s):  
Immunosorbent Assays ◽  
Mumps Antibodies

Development of enzyme-linked immunosorbent assays for the determination of N1, N12-diacetylspermine in human urine

2020 ◽  
Author(s):  
Junwei Liu ◽  
Jinfeng Wang ◽  
Jin Li ◽  
Mengyao Yang ◽  
Wei Yang ◽  
...  
Keyword(s):  
Human Urine ◽  
Immunosorbent Assays

Evaluation of Enzyme-Linked Immunosorbent Assays (ELISAs) for the Determination of Microcystins in Cyanobacteria

2012 ◽  
Vol 13 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Fatma Gurbuz ◽  
James S. Metcalf ◽  
Geoffrey A. Codd ◽  
Aynur G. Karahan
Keyword(s):  
Immunosorbent Assays

Determination of synapsin I and synaptophysin in body fluids by two-site enzyme-linked immunosorbent assays

1998 ◽  
Vol 213 (2) ◽  
pp. 191-199 ◽  
Author(s):  
Gerald Schlaf ◽  
Claudia Salje ◽  
Astrid Wetter ◽  
Kristin Stuertz ◽  
Klaus Felgenhauer ◽  
...  
Keyword(s):  
Body Fluids ◽  
Synapsin I ◽  
Immunosorbent Assays

Comparison of Two ELISA Screening Tests with Liquid Chromatography for Determination of Aflatoxins in Raw Peanuts

1989 ◽  
Vol 72 (6) ◽  
pp. 962-964
Author(s):  
Joe W Dorner ◽  
Richard J Cole
Keyword(s):  
Liquid Chromatography ◽  
Detection Threshold ◽  
Screening Tests ◽  
Rapid Screening ◽  
Detection Thresholds ◽  
Immunosorbent Assays ◽  
Card Test

Abstract A study was conducted to evaluate the performance of 2 enzymelinked immunosorbent assays (ELISA) for rapidly screening samples of peanuts for the presence of aflatoxin. The EZ-Screen Quick Card Test and the Afla-10 Cup Test were compared with liquid chromatography in duplicate analyses of common extracts of peanuts contaminated in the range of 0-70 ppb (ng/g). Each assay properly identified 95% of samples containing no detectable aflatoxin as negative and >97% of samples containing >10 ppb aflatoxin as positive. The card test, which had a 20 ppb detection threshold, identified as positive 32 of 34 samples in the 11-20 ppb range. This indicates that the card test might actually have a detection threshold closer to 10 ppb. Most of the errors associated with the assays occurred on samples containing <10 ppb aflatoxin. The cup and card tests identified 76 and 67% of the samples, respectively, as negative, in the range of 4-10 ppb. For samples either negative or contaminated above their detection thresholds for the assays, the methods are well suited for use as rapid screening tests.

scholarly journals Comparative Evaluation of Nine Different Enzyme-Linked Immunosorbent Assays for Determination of Antibodies againstTreponema pallidum in Patients with Primary Syphilis

2000 ◽  
Vol 38 (3) ◽  
pp. 1279-1282 ◽  
Author(s):  
Bruno L. Schmidt ◽  
Marzieh Edjlalipour ◽  
Anton Luger
Keyword(s):  
Venereal Disease ◽  
Treponema Pallidum ◽  
High Sensitivity ◽  
Immunoglobulin M ◽  
Routine Testing ◽  
Primary Syphilis ◽  
Immunosorbent Assays ◽  
Late Stages ◽  
Higher Sensitivity

Nine different enzyme-linked immunosorbent assays (ELISAs) with a sonicate or recombinant proteins of Treponema pallidum as antigen have been evaluated comparatively by testing 52 highly selected sera from patients with primary syphilis, all negative in the microhemagglutination test for T. pallidum (MHA-TP). Eight tests exhibited greater sensitivity (48.5 to 76.9%) than the commonly used Venereal Disease Research Laboratory test (44.2%). Higher sensitivity could be related to (i) the volume and dilution of the serum, (ii) the design of the assay (capture and competitive tests showed higher sensitivity than sandwich-based assays), and (iii) the ability to detected specific immunoglobulin M antibodies. The specificity of the ICE Syphilis and the Enzygnost Syphilis tests was 99.5 and 99.8%, respectively, as determined by routine testing of 2,053 unselected sera in comparison with the MHA-TP test. ELISAs tested offered high sensitivity in patients with primary syphilis; however, recommendations to use these tests as screening assays do need further data on specificity and reactivity in late stages of the disease.

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