Inhibitory effects of chloramphenicol isomers and other antibiotics on protein synthesis and respiration in procyclic Trypanosoma brucei brucei

1981 ◽  
Vol 2 (3-4) ◽  
pp. 235-255 ◽  
Author(s):  
Terence W. Spithill ◽  
Susan P. Shimer ◽  
George C. Hill
Keyword(s):  
Protein Synthesis ◽  
Trypanosoma Brucei ◽  
Inhibitory Effects ◽  
Trypanosoma Brucei Brucei

scholarly journals Characterization of Trypanosoma brucei bruceiS-adenosyl-l-methionine decarboxylase and its inhibition by Berenil, pentamidine and methylglyoxal bis(guanylhydrazone)

1986 ◽  
Vol 237 (3) ◽  
pp. 685-689 ◽  
Author(s):  
A J Bitonti ◽  
J A Dumont ◽  
P P McCann
Keyword(s):  
Trypanosoma Brucei ◽  
Decarboxylase Activity ◽  
Inhibitory Effects ◽  
Trypanosoma Brucei Brucei ◽  
Curative Effect ◽  
Model Mouse ◽  
Mammalian Enzyme

Trypanosoma brucei brucei S-adenosyl-L-methionine (AdoMet) decarboxylase was found to be relatively insensitive to activation by putrescine as compared with the mammalian enzyme, being stimulated by only 50% over a 10,000-fold range of putrescine concentrations. The enzyme was not stimulated by up to 10 mM-Mg2+. The Km for AdoMet was 30 microM, similar to that of other eukaryotic AdoMet decarboxylases. T.b. brucei AdoMet decarboxylase activity was apparently irreversibly inhibited in vitro by Berenil and reversibly by pentamidine and methylglyoxal bis(guanylhydrazone). Berenil also inhibited trypanosomal AdoMet decarboxylase by 70% within 4 h after administration to infected rats and markedly increased the concentration of putrescine in trypanosomes that were exposed to the drug in vivo. Spermidine and spermine blocked the curative effect of Berenil on model mouse T.b. brucei infections. This effect of the polyamines was probably not due to reversal of Berenil's inhibitory effects on the AdoMet decarboxylase.

scholarly journals Effects of α-difluoromethylornithine on protein synthesis and synthesis of the variant-specific glycoprotein (VSG) in Trypanosoma brucei brucei

1988 ◽  
Vol 250 (1) ◽  
pp. 295-298 ◽  
Author(s):  
A J Bitonti ◽  
D E Cross-Doersen ◽  
P P McCann
Keyword(s):  
Protein Synthesis ◽  
Trypanosoma Brucei ◽  
Mechanism Of Action ◽  
Myristic Acid ◽  
Leucine Incorporation ◽  
Biochemical Mechanism ◽  
Trypanosoma Brucei Brucei ◽  
Apparent Decrease

Protein synthesis in Trypanosoma brucei brucei was rapidly inhibited during polyamine depletion by DL-alpha-difluoromethylornithine (DFMO) in vitro and in vivo. [3H]Leucine incorporation was depressed 30-40% by 24 h and 80-90% by 48 h of DFMO treatment. Concomitantly there was an apparent decrease in the synthesis of the variant-specific glycoprotein (VSG) in DFMO-treated trypanosomes, as measured by decreased incorporation of [3H]myristic acid into VSG. The discovery of decreased protein synthesis in T. b. brucei during DFMO treatment is noteworthy, because it was reported previously that protein synthesis was paradoxically stimulated 2-4-fold during DFMO treatment in these organisms. Decreased protein synthesis probably relates to the biochemical mechanism of action of DFMO on trypanosomes.

Effects of n-hexane and ethylacetate fractions of Terminalia catappa leaf extract in experimental Trypanosoma brucei brucei infection in Wistar rats

2021 ◽  
Author(s):  
Folashade Sarah Ojeleye ◽  
Helen Ileigo Inabo ◽  
Clement Myah Zaman Whong ◽  
Bolanle Olufunke Priscilla Musa ◽  
Ochuko Orakpoghenor
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Trypanosoma Brucei Brucei ◽  
Terminalia Catappa

Modulation of Early Immune Responses and Suppression of Trypanosoma brucei brucei Infections by Surgical Denervation of the Spleen

2000 ◽  
Vol 8 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Yajuan Liu ◽  
Manal Mustafa ◽  
Hu-Lun Li ◽  
Lauri Nuortio ◽  
Amged Mustafa ◽  
...  
Keyword(s):  
Immune Responses ◽  
Trypanosoma Brucei ◽  
Trypanosoma Brucei Brucei
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