Effects of n-hexane and ethylacetate fractions of Terminalia catappa leaf extract in experimental Trypanosoma brucei brucei infection in Wistar rats

2021 ◽  
Author(s):  
Folashade Sarah Ojeleye ◽  
Helen Ileigo Inabo ◽  
Clement Myah Zaman Whong ◽  
Bolanle Olufunke Priscilla Musa ◽  
Ochuko Orakpoghenor
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Trypanosoma Brucei Brucei ◽  
Terminalia Catappa

scholarly journals Effect of Leaf Extract of Senna alata on Biochemical Indices of Wistar Rats Infected with Trypanosoma brucei brucei

2020 ◽  
pp. 10-19
Author(s):  
M. A. Kugama ◽  
T. Tese ◽  
H. Sabo ◽  
T. Andrew ◽  
Y. A. Onaolapo ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Potassium Concentration ◽  
Sodium Concentration ◽  
Chloroform Extract ◽  
Trypanosoma Brucei Brucei ◽  
Infection Treatment ◽  
Biochemical Indices ◽  
Senna Alata

This study was aimed at determining the effect of leaves of Senna alata extract on biochemical indices of Wistar rats infected with Trypanosoma brucei brucei. Phytochemical screening revealed the absence of steroids in all extracts, absence of saponins in chloroform extracts and the presence of free anthraquinones only in chloroform extract. Post-infection treatment of animals stirred the emergence of parasitaemia by Day 3. Only animals receiving 200 mg/kg b.wt. of chloroform extract survived by day 16. A significant (P<0.05) decrease in ALT for groups receiving methanol (400 mg/kg b.wt.), chloroform and aqueous extracts and significant (P<0.05) increase in unconjugated bilirubin in the group receiving methanol extract (200 mg/kg b.wt.) compared to infected not treated rats. Significant (P<0.05) decrease in potassium concentration in groups receiving methanol and chloroform, and a significant (P<0.05) increase in sodium concentration in the group receiving 400 mg/kg b.wt. of aqueous extract compared to the infected not treated rats. These results thereby demonstrate the ameliorative potential of Senna alata leaves against T. brucei brucei.

Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats

2014 ◽  
Vol 96 (1) ◽  
pp. 139-142 ◽  
Author(s):  
Patricia I. Kobo ◽  
Joseph O. Ayo ◽  
Tagang Aluwong ◽  
Abdulkadir U. Zezi ◽  
Victor Maikai ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Osmotic Fragility ◽  
Trypanosoma Brucei Brucei ◽  
Erythrocyte Osmotic Fragility

scholarly journals Trypanocidal Potentials of Azadirachta indica: In Vivo Activity of Leaf Extract against Trypanosoma brucei brucei.

1993 ◽  
Vol 15 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Andrew J. NOK ◽  
King A.N. ESIEVO ◽  
Ishaya LONGDET ◽  
Samuel AROWOSAFE ◽  
Paul C. ONYENEKWE ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Trypanosoma Brucei Brucei

scholarly journals The Role of Serum Alpha-Amylase and Glycogen Synthase in the Anti-Diabetic Potential of Terminalia catappa Aqueous Leaf Extract in Diabetic Wistar Rats

2019 ◽  
pp. 1-11
Author(s):  
Ezekiel E. Ben ◽  
Asuquo E. Asuquo ◽  
D. U. Owu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Glycogen Synthase ◽  
Alpha Amylase ◽  
Distilled Water ◽  
Terminalia Catappa ◽  
Aqueous Leaf Extract

Background: The endocrinal abnormalities in diabetes mellitus as one of the numerous metabolic disorders is associated with derangement in exocrine functions of the pancreas and ultimately influences blood glucose regulation. Aim: The study was aimed at assessing the role of alpha-amylase and glycogen synthase in anti-diabetic potential of Terminalia catappa in diabetic rats.  Materials and Methods: Thirty five (35) Wistar rats were assigned to 5 groups of 7 animals each. Group 1 served as the control administered distilled water at 5ml/kg bodyweight and group 2 was a non diabetic group given orally, 130/kg body weight of aqueous leaf extract of Terminalia catappa.  Groups 3, 4 and 5 received a single dose of 150mg/kg body weight of alloxan solution intraperitoneally to induce diabetes and rats with blood glucose levels ≥200mg/dl after 72 hours were considered diabetic. This was followed by oral administration of 5ml/kg bodyweight of distilled water, 130mg/kg body weight of Terminalia catappa leaf extract orally and subcutaneous administration of insulin, 0.75U/kg body weight to groups 3 (diabetic), 4(diabetic + extract) and 5 (diabetic + insulin) respectively. Results: The results showed significant (P<.05) increase in serum level of alpha-amylase and glycogen synthase in both non-diabetic extract treated and diabetic groups when compared to control. But these enzymes significantly (P<.05) reduced in diabetic extract and insulin treated groups when compared to the diabetic group.  Conclusion: Therefore the hypoglycaemic potential of Terminalia catappa leaf extract could be attributed to its ability to reduce alpha-amylase level while lowered glycogen synthase might be secondary to reduction in blood glucose.

scholarly journals Haematological changes in Wistar rats experimentally infected with Trypanosoma congolense and Trypanosoma brucei brucei obtained from North-west Nigeria

2021 ◽  
Vol 19 (2) ◽  
pp. 73-80
Author(s):  
A.O. Fajinmi ◽  
O.O. Faleke ◽  
A.A. Magaji ◽  
U.M. Chafe ◽  
M.A. Kassim ◽  
...  
Keyword(s):  
Blood Cell ◽  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Trypanosoma Congolense ◽  
Control Group ◽  
Blood Cell Count ◽  
Trypanosoma Brucei Brucei ◽  
Group A ◽  
Haematological Changes ◽  
Group D

This study determined haematological changes in Wistar rats experimentally infected with local strains of Trypanosoma congolense and Trypanosoma brucei brucei. Forty-five Wistar rats between 10 – 12 weeks old weighing between 210 – 240 g were used. The Wistar rats were randomly divided into four groups (A, B, C and D), with the infected groups (B, C and D) having 10 rats each, while the uninfected control group (A) had 15 rats. Group A rats were not infected and served as the control, group B were infected with Trypanosoma congolense, group C were infected with Trypanosoma brucei brucei and group D were co-infected with Trypanosoma congolense and Trypanosoma brucei brucei. Infection was achieved using 0.1mL of blood containing approximately 1 × 103 trypanosomes intraperitoneally into each Wistar rat in the infected groups. Clinical signs were observed. The changes in the blood cells were assayed in the groups post-infection. Duncan’s Least Square Deviation showed significantly (p<0.05) higher parasitaemia in infected groups. However, group D showed a higher significant (p<0.05) difference in parasitaemia when compared to groups B and C. The pattern of mean parasitaemia for the infected groups, revealed a positive correlation with days of post-infection (p<0.05) before the decline. The packed cell volume, total red blood cell count and haemoglobin concentration were significantly (p<0.05) lower in infected groups B, C and D. The total white blood cell count, platelet counts and differential leucocyte count were significantly (p<0.05) lower in infected groups when compared to the uninfected group. These findings suggest that co-infection with Trypanosoma congolense and Trypanosoma brucei brucei obtained from Wurno and Ngaski in Sokoto and Kebbi States respectively produced a more damaging effect on haematological parameters.

In vivo trypanocidal activity of Annona senegalensis Pers. leaf extract against Trypanosoma brucei brucei

2007 ◽  
Vol 112 (1) ◽  
pp. 85-89 ◽  
Author(s):  
Emmanuel O. Ogbadoyi ◽  
Akinsunbo O. Abdulganiy ◽  
Theophilus Z. Adama ◽  
Joseph I. Okogun
Keyword(s):  
Trypanosoma Brucei ◽  
Leaf Extract ◽  
Trypanosoma Brucei Brucei ◽  
Trypanocidal Activity

scholarly journals In vitro assay and in vivo effect of artemisinin in Trypanosoma brucei brucei-infected Wistar rats

2021 ◽  
Vol 1 (3) ◽  
pp. 100061
Author(s):  
Kelvin Olutimilehin Jolayemi ◽  
Mohammed Mamman ◽  
Dahiru Sani ◽  
Magdalene Ogbonneya Okoronkwo ◽  
Abubakar Usman ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
In Vitro Assay ◽  
Trypanosoma Brucei Brucei ◽  
Vitro Assay
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