Singlet oxygen induced single-strand breaks in plasmid pBR322 DNA: the enhancing effect of thiols

1991 ◽  
Vol 1088 (3) ◽  
pp. 409-412 ◽  
Author(s):  
Thomas P.A. Devasagayam ◽  
Paolo Di Mascio ◽  
Stephan Kaiser ◽  
Helmut Sies
Keyword(s):  
Singlet Oxygen ◽  
Single Strand ◽  
Plasmid Pbr322 ◽  
Strand Breaks ◽  
Enhancing Effect ◽  
Single Strand Breaks ◽  
Pbr322 Dna

Determination of action spectrum for sparfloxacin-photosensitized single-strand breaks in plasmid pBR322 DNA

2005 ◽  
Vol 21 (6) ◽  
pp. 287-292 ◽  
Author(s):  
Katsuhide Sayama ◽  
Yuki Kobayashi ◽  
Hitoshi Fujita ◽  
Atsushi Ito ◽  
Yoshiki Tokura ◽  
...  
Keyword(s):  
Action Spectrum ◽  
Single Strand ◽  
Plasmid Pbr322 ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Pbr322 Dna

Protection of plasmid pBR322 DNA by flavonoids against single-strand breaks induced by singlet molecular oxygen

1995 ◽  
Vol 30 (2-3) ◽  
pp. 97-103 ◽  
Author(s):  
Thomas P.A. Devasagayam ◽  
Mayilvahanan Subramanian ◽  
B.B. Singh ◽  
R. Ramanathan ◽  
Nagaratnam P. Das
Keyword(s):  
Molecular Oxygen ◽  
Single Strand ◽  
Plasmid Pbr322 ◽  
Singlet Molecular Oxygen ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Pbr322 Dna

Photocleavage of Plasmid pBR322 DNA by Some Anionic Porphyrins

1998 ◽  
Vol 02 (04) ◽  
pp. 337-343 ◽  
Author(s):  
SHAMPA R. CHATTERJEE ◽  
T. S. SRIVASTAVA ◽  
J. P. KAMAT ◽  
T. P. A. DEVASAGAYAM
Keyword(s):  
Lipoic Acid ◽  
Plasmid Dna ◽  
Dna Cleavage ◽  
Irradiation Time ◽  
Water Soluble ◽  
Plasmid Pbr322 ◽  
Limited Extent ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Pbr322 Dna

The water-soluble porphyrins meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin ( H 2 T4CPP ), meso-tetrakis[3-(carboxymethyleneoxy)phenyl]porphyrin ( H 2 T3CPP ) and meso-tetrakis[3,4-bis(carboxymethyleneoxy)phenyl]porphyrin ( H 2 T3 , 4BCPP ) cleave plasmid pBR322 DNA to single-strand breaks (SSBs) in the presence of molecular oxygen and visible light. These porphyrins induced SSBs in DNA as a function of irradiation time as well as porphyrin concentration. Under similar conditions (10 μM or more), H 2 T3CPP showed more SSBs in DNA than the porphyrins H 2 T 4CPP and H 2 T 3,4 BCPP . The DNA cleavage was more in D 2 O -based buffer than in H 2 O buffer. In addition, this DNA cleavage was inhibited by the presence of sodium azide and lipoic acid, which are potent quenchers of singlet oxygen (1 O 2). These observations suggest the involvement of 1 O 2 in photocleavage of DNA. Further, the DNA cleavage, to a limited extent, was also inhibited by tert-butanol and mannitol, both quenchers of hydroxyl radical (· OH ), suggesting the involvement of · OH in photocleavage of DNA. Thus both 1 O 2 and · OH are involved in photocleavage of plasmid DNA by these porphyrins.

PHOTOSENSITIZATION OF SINGLE-STRAND BREAKS IN pBR322 DNA BY ROSE BENGAL

1989 ◽  
Vol 49 (3) ◽  
pp. 293-298 ◽  
Author(s):  
THOMAS A. CIULLA ◽  
JOHN R. CAMP ◽  
EVAN ROSENFELD ◽  
IRENE E. KOCHEVAR
Keyword(s):  
Rose Bengal ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Pbr322 Dna
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