Relationship between TGFα-induced DNA synthesis and prostaglandin synthesis in human HaCaT keratinocytes

Cultured pneumocytes, prepared from fetal rat lung, are growth inhibited and have increased lactate dehydrogenase release and prostaglandin synthesis in response to 50 and 95% O2 exposure. The uptake of cationic liposomes by these fetal cells is more rapid and extensive than is the case with cultured adult pneumocytes. Protection of fetal pneumocytes against the cytotoxic effects of 50 or 95% O2 by liposome-entrapped antioxidant enzymes requires a liposome phospholipid concentration of only 1 nmol/cm2, compared with 45 nmol/cm2 for adult cells, which is a cytotoxic phospholipid concentration for the fetal cells. Despite this capacity of low concentrations of liposomes containing superoxide dismutase and catalase to increase endogenous antioxidant enzyme content, and to protect against cell death, such treatment does not attenuate O2-mediated alterations of cell growth or prostaglandin release. Inhibition of pneumocyte DNA synthesis, by elevated O2 concentrations, cannot be attributed to an autocrine effect of enhanced prostaglandin synthesis, because the addition of 50 microM ibuprofen to inhibit prostaglandin synthesis does not prevent O2-mediated effects on DNA synthesis.

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Intracellular calcium pool emptying induces DNA synthesis in HaCaT keratinocytes

Experimental Dermatology ◽

10.1034/j.1600-0625.2003.00013.x ◽

2003 ◽

Vol 12 (4) ◽

pp. 453-459 ◽

Cited By ~ 8

Author(s):

R. Gniadecki ◽

B. Gajkowska

Keyword(s):

Dna Synthesis ◽

Intracellular Calcium ◽

Hacat Keratinocytes ◽

Calcium Pool

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DNA synthesis in cultured human keratinocytes and HaCaT keratinocytes is reduced by specific inhibition of dipeptidylpeptidase IV (CD26) activity

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)83496-8 ◽

1998 ◽

Vol 16 ◽

pp. S83

Author(s):

R. Vetter ◽

D. Reinhold ◽

F. Bühling ◽

S. Ansorge ◽

H. Gollnick

Keyword(s):

Dna Synthesis ◽

Human Keratinocytes ◽

Specific Inhibition ◽

Hacat Keratinocytes ◽

Dipeptidylpeptidase Iv

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DNA synthesis in HaCaT keratinocytes is reduced by specific inhibitors of dipeptidylpeptidase IV (CD26)

Journal of the European Academy of Dermatology and Venereology ◽

10.1016/s0926-9959(98)95542-8 ◽

1998 ◽

Vol 11 ◽

pp. S255

Author(s):

R VETTER

Keyword(s):

Dna Synthesis ◽

Hacat Keratinocytes ◽

Dipeptidylpeptidase Iv ◽

Specific Inhibitors

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Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002385 ◽

1980 ◽

Vol 38 ◽

pp. 540-541

Author(s):

Dwight Anderson ◽

Charlene Peterson ◽

Gursaran Notani ◽

Bernard Reilly

Keyword(s):

Electron Microscopy ◽

Bacillus Subtilis ◽

Dna Synthesis ◽

Restriction Endonuclease ◽

Protein Complex ◽

Protein Product ◽

Viral Dna ◽

Small Proteins ◽

Antibody Labeling ◽

Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

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