Gene expression of urea cycle enzymes following two-thirds partial hepatectomy in the rat

1995 ◽  
Vol 22 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Niels Tygstrup ◽  
Søren Bak ◽  
Bjørg Krog ◽  
Antonello Pietrangelo ◽  
David A. Shafritz
Keyword(s):  
Gene Expression ◽  
Partial Hepatectomy ◽  
Urea Cycle ◽  
Urea Cycle Enzymes

scholarly journals Subzero nonfreezing storage of rat hepatocytes using UW solution and 1,4-butanediol. II- functional testing on rewarming and gene expression of urea cycle enzymes

2009 ◽  
Vol 8 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Edgardo E. Guibert ◽  
Luciana L. Almada ◽  
Maria E. Mamprin ◽  
Cristina Bellarosa ◽  
Maria Dolores Pizarro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Urea Cycle ◽  
Rat Hepatocytes ◽  
Functional Testing ◽  
Uw Solution ◽  
Urea Cycle Enzymes

Gene expression and activity of urea cycle enzymes of rat hepatocytes cold stored up to 120h in University of Wisconsin solution

Cryobiology ◽  
2006 ◽  
Vol 52 (3) ◽  
pp. 393-400 ◽  
Author(s):  
Luciana Almada ◽  
Cristina Bellarosa ◽  
Pablo Giraudi ◽  
María Mamprín ◽  
María Mediavilla ◽  
...  
Keyword(s):  
Gene Expression ◽  
Urea Cycle ◽  
Rat Hepatocytes ◽  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Wisconsin Solution ◽  
Expression And Activity ◽  
Urea Cycle Enzymes

scholarly journals The urea cycle of rat white adipose tissue

RSC Advances ◽  
2015 ◽  
Vol 5 (113) ◽  
pp. 93403-93414 ◽  
Author(s):  
Sofía Arriarán ◽  
Silvia Agnelli ◽  
Xavier Remesar ◽  
José-Antonio Fernández-López ◽  
Marià Alemany
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
High Activity ◽  
White Adipose Tissue ◽  
Urea Cycle ◽  
Catalytic Capacity ◽  
Urea Cycle Enzymes

White adipose tissue urea-cycle enzymes showed a high activity and gene expression, second only to liver in catalytic capacity.

scholarly journals Carnitine administration to juvenile visceral steatosis mice corrects the suppressed expression of urea cycle enzymes by normalizing their transcription.

1992 ◽  
Vol 267 (8) ◽  
pp. 5032-5035
Author(s):  
M Horiuchi ◽  
K Kobayashi ◽  
M Tomomura ◽  
M Kuwajima ◽  
Y Imamura ◽  
...  
Keyword(s):  
Urea Cycle ◽  
Urea Cycle Enzymes

Hepatocellular expression of glucose-6-phosphatase is unaltered during hepatic regeneration

1998 ◽  
Vol 275 (4) ◽  
pp. G717-G722 ◽  
Author(s):  
Wisam F. Zakko ◽  
Carl L. Berg ◽  
John L. Gollan ◽  
Richard M. Green
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Protein Expression ◽  
Partial Hepatectomy ◽  
Initial Phase ◽  
Physiological Function ◽  
Liver Homogenate ◽  
Hepatic Regeneration ◽  
Mrna Levels ◽  
Microsomal Enzyme

Gluconeogenesis and glycogenolysis are essential hepatic functions required for glucose homeostasis. During the initial phase of hepatic regeneration, the immediate-early genes (IEG) are rapidly expressed, and the IEG RL-1 encodes for glucose-6-phosphatase (G-6- Pase). G-6- Pase is a microsomal enzyme essential for gluconeogenesis and glycogenolysis. This study employs a partial-hepatectomy model to examine the expression and activity of G-6- Pase. After partial hepatectomy, rat hepatic G-6- Pase gene expression is transcriptionally regulated, and mRNA levels are increased ≈30-fold. However, in contrast to this rapid gene induction, microsomal enzyme activity is unchanged after partial hepatectomy. Western blotting demonstrates that microsomal G-6- Pase protein expression is also unchanged after partial hepatectomy, and similar results are also noted in whole liver homogenate. Thus, despite marked induction in gene expression of the IEG G-6- Pase after partial hepatectomy, protein expression and enzyme activity remain unchanged. These data indicate that, although this hepatocyte IEG is transcriptionally regulated, the physiologically important level of regulation is posttranscriptional. This highlights the importance of correlating gene expression of IEG with protein expression and physiological function.

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