ENZEDUC 1.0: a user-friendly software package for computation of hyperbolic enzyme kinetic data in biochemical education

1996 ◽  
Vol 49 (2) ◽  
pp. 131-135
Author(s):  
Aida Marino ◽  
JoséR. Fedriani
Keyword(s):  
Software Package ◽  
Kinetic Data ◽  
Enzyme Kinetic ◽  
User Friendly

scholarly journals Treatment of Enzyme Kinetic Data

1967 ◽  
Vol 242 (18) ◽  
pp. 4045-4052 ◽  
Author(s):  
Carl Frieden
Keyword(s):  
Kinetic Data ◽  
Enzyme Kinetic

A user-friendly general-purpose predictive software package for food safety

2011 ◽  
Vol 104 (2) ◽  
pp. 173-185 ◽  
Author(s):  
Amit Halder ◽  
Ashish Dhall ◽  
Ashim K. Datta ◽  
D. Glenn Black ◽  
P.M. Davidson ◽  
...  
Keyword(s):  
Food Safety ◽  
Software Package ◽  
General Purpose ◽  
User Friendly ◽  
Predictive Software

A General-Purpose Software for Menu Design of CAD Projects

1993 ◽  
Author(s):  
Y. C. Pao
Keyword(s):  
Software Package ◽  
Computer Programs ◽  
General Purpose ◽  
Final Report ◽  
Sample Design ◽  
Main Menu ◽  
Sample Application ◽  
User Friendly ◽  
Four Bar Linkage ◽  
General Purpose Software

Abstract A software package MenuCAD has been developed for the general need of designing menu-driven, user-friendly CAD computer programs. The main menu is formatted similar to the major contents in the final report of the design project including Contents, Analysis, Sample Design Cases, Illustrations and Tables, References, and Program Listings. Sub-menus are further divided into items delineating the steps involved in the design. Screen help messages are provided for design of the main menu and sub-menus interactively and for applying the arrow keys on the keyboard to select a sub-menus and a particular item in the sub-menu in order to execute a desired design step. MenuCAD builds the framework, its user has to supplement with a subroutine ExecItem for describing the special features and for directing how each design step should be executed in the project. A CAD design of four-bar linkage project is presented as a sample application of this package.

scholarly journals The quantitative analysis of ligand binding and initial-rate data for allosteric and other complex enzyme mechanisms

1976 ◽  
Vol 153 (1) ◽  
pp. 101-117 ◽  
Author(s):  
W G Bardsley
Keyword(s):  
Kinetic Data ◽  
Quantitative Data ◽  
Initial Rate ◽  
Graphical Methods ◽  
Enzyme Kinetic ◽  
Rate Data ◽  
Curve Shape ◽  
High Substrate Concentration ◽  
Complex Curve ◽  
Complex Curves

1. The eight methods for plotting enzyme kinetic data are classified and analysed, and it is shown how, in each case, it is only possible to obtain quantitative data on the coefficients of the lowest- and highest-degree terms in the rate equation. 2. The combinations of coefficients that are accessible experimentally from limiting slopes and intercepts at both low and high substrate concentration are stated for all the graphical methods and the precise effects of these on curve shape in different spaces is discussed. 3. Ambiguities arising in the analysis of complex curves and certain special features are also investigated. 4. Four special ordering functions are defined and investigated and it is shown how knowledge of these allows a complete description of all possible complex curve shapes.

Direct Measurement of Iodine Production by Sonic Extracts of Polymorphonuclear Leukocytes

1971 ◽  
Vol 17 (5) ◽  
pp. 392-396 ◽  
Author(s):  
Lawrence R DeChatelet ◽  
Charles E McCall ◽  
M Robert Cooper
Keyword(s):  
Absorption Spectrum ◽  
Kinetic Data ◽  
Polymorphonuclear Leukocytes ◽  
Enzymatic Reaction ◽  
Human Neutrophils ◽  
Enzyme Kinetic ◽  
Ph Optimum ◽  
Beef Liver ◽  
Free Iodine ◽  
High Concentrations

Abstract We describe an enzymatic reaction between iodide ion, H2O2, and neutrophil sonicates, in which free iodine is formed. Some characteristics of the reaction are: (a) it is catalyzed by sonic extracts of human neutrophils, by purified horseradish peroxidase, or purified human myeloperoxidase, but not by sonic extracts of rabbit alveolar macrophages or beef liver catalase; (b) iodine is the product, as shown by its absorption spectrum and the absorption spectrum of the starch adduct; (c) the reaction is proportional to the amount of neutrophil sonicate added, and has a pH optimum near 4.0. Reaction is not linear with respect to time, owing to denaturation of the enzyme. Kinetic data indicate that the enzyme may be allosteric with respect to iodide and is inhibited by high concentrations of H2O2. These represent possible sources of control of the reaction.

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