Some Complex Kinetic Mechanisms and Treatment of Enzyme Kinetic Data

2019 ◽  
pp. 341-376
Author(s):  
Stephen A. Kuby
Keyword(s):  
Kinetic Data ◽  
Enzyme Kinetic ◽  
Kinetic Mechanisms

scholarly journals Treatment of Enzyme Kinetic Data

1967 ◽  
Vol 242 (18) ◽  
pp. 4045-4052 ◽  
Author(s):  
Carl Frieden
Keyword(s):  
Kinetic Data ◽  
Enzyme Kinetic

scholarly journals The quantitative analysis of ligand binding and initial-rate data for allosteric and other complex enzyme mechanisms

1976 ◽  
Vol 153 (1) ◽  
pp. 101-117 ◽  
Author(s):  
W G Bardsley
Keyword(s):  
Kinetic Data ◽  
Quantitative Data ◽  
Initial Rate ◽  
Graphical Methods ◽  
Enzyme Kinetic ◽  
Rate Data ◽  
Curve Shape ◽  
High Substrate Concentration ◽  
Complex Curve ◽  
Complex Curves

1. The eight methods for plotting enzyme kinetic data are classified and analysed, and it is shown how, in each case, it is only possible to obtain quantitative data on the coefficients of the lowest- and highest-degree terms in the rate equation. 2. The combinations of coefficients that are accessible experimentally from limiting slopes and intercepts at both low and high substrate concentration are stated for all the graphical methods and the precise effects of these on curve shape in different spaces is discussed. 3. Ambiguities arising in the analysis of complex curves and certain special features are also investigated. 4. Four special ordering functions are defined and investigated and it is shown how knowledge of these allows a complete description of all possible complex curve shapes.

Direct Measurement of Iodine Production by Sonic Extracts of Polymorphonuclear Leukocytes

1971 ◽  
Vol 17 (5) ◽  
pp. 392-396 ◽  
Author(s):  
Lawrence R DeChatelet ◽  
Charles E McCall ◽  
M Robert Cooper
Keyword(s):  
Absorption Spectrum ◽  
Kinetic Data ◽  
Polymorphonuclear Leukocytes ◽  
Enzymatic Reaction ◽  
Human Neutrophils ◽  
Enzyme Kinetic ◽  
Ph Optimum ◽  
Beef Liver ◽  
Free Iodine ◽  
High Concentrations

Abstract We describe an enzymatic reaction between iodide ion, H2O2, and neutrophil sonicates, in which free iodine is formed. Some characteristics of the reaction are: (a) it is catalyzed by sonic extracts of human neutrophils, by purified horseradish peroxidase, or purified human myeloperoxidase, but not by sonic extracts of rabbit alveolar macrophages or beef liver catalase; (b) iodine is the product, as shown by its absorption spectrum and the absorption spectrum of the starch adduct; (c) the reaction is proportional to the amount of neutrophil sonicate added, and has a pH optimum near 4.0. Reaction is not linear with respect to time, owing to denaturation of the enzyme. Kinetic data indicate that the enzyme may be allosteric with respect to iodide and is inhibited by high concentrations of H2O2. These represent possible sources of control of the reaction.

scholarly journals An artificial-intelligence technique for qualitatively deriving enzyme kinetic mechanisms from initial-velocity measurements and its application to hexokinase

1989 ◽  
Vol 264 (1) ◽  
pp. 175-184 ◽  
Author(s):  
L Garfinkel ◽  
D M Cohen ◽  
V W Soo ◽  
D Garfinkel ◽  
C A Kulikowski
Keyword(s):  
Artificial Intelligence ◽  
Experimental Data ◽  
Ionic Strength ◽  
Initial Velocity ◽  
Large Data ◽  
Data Sets ◽  
Enzyme Kinetic ◽  
Experimental Conditions ◽  
Enzyme Preparations ◽  
Kinetic Mechanisms

We have developed a computer method based on artificial-intelligence techniques for qualitatively analysing steady-state initial-velocity enzyme kinetic data. We have applied our system to experiments on hexokinase from a variety of sources: yeast, ascites and muscle. Our system accepts qualitative stylized descriptions of experimental data, infers constraints from the observed data behaviour and then compares the experimentally inferred constraints with corresponding theoretical model-based constraints. It is desirable to have large data sets which include the results of a variety of experiments. Human intervention is needed to interpret non-kinetic information, differences in conditions, etc. Different strategies were used by the several experimenters whose data was studied to formulate mechanisms for their enzyme preparations, including different methods (product inhibitors or alternate substrates), different experimental protocols (monitoring enzyme activity differently), or different experimental conditions (temperature, pH or ionic strength). The different ordered and rapid-equilibrium mechanisms proposed by these experimenters were generally consistent with their data. On comparing the constraints derived from the several experimental data sets, they are found to be in much less disagreement than the mechanisms published, and some of the disagreement can be ascribed to different experimental conditions (especially ionic strength).

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