Polymorphic markers of human T-cell receptor alpha and beta genes. Family studies and comparison of frequencies in healthy individuals and patients with multiple sclerosis and myasthenia gravis

A cDNA clone encoding the alpha chain of the human T cell receptor was used in connection with somatic cell human-rodent hybrids to determine that the genes coding for the alpha chain are located on chromosome 14 in humans. In situ hybridization confirms this result and further localizes these genes to 14q11-14q12 on this chromosome. Since this region of chromosome has been shown to be nonrandomly involved in a number of T cell neoplasias, this assignment raises a number of interesting questions as to the possible involvement of the T cell receptor alpha chain genes in tumorigenesis.

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Discordance of the T-cell receptor alpha-chain gene in familial multiple sclerosis

Neurology ◽

10.1212/wnl.42.4.839 ◽

1992 ◽

Vol 42 (4) ◽

pp. 839-839 ◽

Cited By ~ 28

Author(s):

S. G. Lynch ◽

J. W. Rose ◽

J. H. Petajan ◽

M. Leppert

Keyword(s):

Multiple Sclerosis ◽

T Cell ◽

T Cell Receptor ◽

Cell Receptor ◽

Chain Gene ◽

Receptor Alpha ◽

Alpha Chain ◽

Receptor Alpha Chain ◽

Cell Receptor Alpha

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The Human T Cell Receptor Alpha Variable (TRAV) Genes

Experimental and Clinical Immunogenetics ◽

10.1159/000019128 ◽

2000 ◽

Vol 17 (2) ◽

pp. 83-96 ◽

Cited By ~ 30

Author(s):

Dominique Scaviner ◽

Marie-Paule Lefranc

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Receptor ◽

Receptor Alpha ◽

Human T Cell ◽

Cell Receptor Alpha

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Sequences and repertoire of human T cell receptor alpha chain variable region genes in mature T lymphocytes.

Journal of Experimental Medicine ◽

10.1084/jem.164.1.90 ◽

1986 ◽

Vol 164 (1) ◽

pp. 90-103 ◽

Cited By ~ 78

Author(s):

Y Yoshikai ◽

N Kimura ◽

B Toyonaga ◽

T W Mak

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Receptor ◽

Common Mechanism ◽

Receptor Alpha ◽

Alpha Chain ◽

Human T Cell ◽

Alpha Gene ◽

Receptor Alpha Chain ◽

Cell Receptor Alpha

24 human T cell receptor alpha chain messages have been examined by cDNA sequence analysis and Southern blot. The data indicate that there are approximately 40 alpha chain T cell receptor variable gene segments, which can be divided into 12 families. Comparison of the J gene segments from the cDNAs to previously determined germline J alpha sequences places the number of J alpha gene segments over 21, and indicates their number to be approximately 55. Identical nucleotide sequences in independent isolates of V alpha and J alpha gene segments indicate that hypermutation may not be a common mechanism for the expansion of diversity in these genes, and suggest that the major source of diversity within the alpha chain repertoire is a result of recombinational joinings between germline V alpha and J alpha sequences, combined with imprecise junctional joining. Analysis of the V regions of these alpha chain messages reveals the presence of three domains of hypervariability roughly analogous to the CDR1, CDR2, and CDR3 regions of immunoglobulin.

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Sizing of the human T cell receptor alpha locus and detection of a large deletion in the Molt-4 cell line

Blood ◽

10.1182/blood.v71.6.1744.1744 ◽

1988 ◽

Vol 71 (6) ◽

pp. 1744-1747 ◽

Cited By ~ 6

Author(s):

MS Roth ◽

FS Collins ◽

D Ginsburg

Keyword(s):

T Cell ◽

Cell Line ◽

T Cell Receptor ◽

Mononuclear Cells ◽

Cell Receptor ◽

Chain Gene ◽

Normal Peripheral Blood ◽

Receptor Alpha ◽

Human T Cell ◽

Cell Receptor Alpha

Abstract The human T cell receptor alpha (TCR-alpha) chain gene consists of discontinuous DNA segments encoding multiple variable (V), multiple joining (J), and one constant (C) region. Unlike other immunoglobulin or TCR genes, however, the TCR-alpha locus exhibits an unusual dispersal of J segments that occupy a region of greater than 50 kilobases (kb) upstream of the C region, with the exact size still unknown. We report here the study of the TCR-alpha genetic locus by using pulsed-field gel electrophoresis (PFGE), which permits the separation of large DNA fragments. Analysis of DNA prepared from normal peripheral blood mononuclear cells, human endothelial cells, and a B cell line demonstrates that both V and C sequences are contained within a single 400-kb SfiI restriction fragment. PFGE analysis of the T cell line Molt 4 suggests a greater than 600-kb deletion involving the TCR- alpha gene.

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