Culture of Bordetella microorganisms - Bordetella pertussis culture medium for whooping cough (pertussis) vaccine production

Vaccine ◽  
1983 ◽  
Vol 1 (1) ◽  
pp. 49-49
Keyword(s):  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Culture Medium ◽  
Whooping Cough ◽  
Vaccine Production

scholarly journals Diphtheria-tetanus-pertussis vaccine combined with Bordetella parapertussis vaccine

1962 ◽  
Vol 60 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Neda Köhler-Kubelka
Keyword(s):  
Clinical Examination ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Production Test ◽  
Post Vaccination ◽  
Bordetella Parapertussis ◽  
Cross Reactions ◽  
Combined Vaccine

Investigations carried out to ascertain the ability of various strains of Bordetella pertussis and B. parapertussis to produce agglutinins have shown that the agglutinin response is considerably greater with B. parapertussis.Children inoculated with a combined vaccine in which the parapertussis element contained B. parapertussis in only one-twelfth of the concentration of B. pertussis in the pertussis element showed agglutinins in their sera in titres well above 1:300 for both organisms. There were no cross-reactions and the serological responses were specific throughout. The vaccine used was the standard diphtheria-tetanus-pertussis (DTP) prophylactic to which had been added a vaccine prepared from recently isolated strains of B. parapertussis.Agglutinin titres of both whooping cough components with the combined vaccine were somewhat lower in mice than was the case when monovalent vaccines were used, but they were considered to be satisfactory.It is suggested that the agglutination production test in mice could be used for the assessment of protective power of B. parapertussis vaccines against infection.I wish to thank Dr Ikić, director of the Institute of Immunology, Zagreb, who enabled me to perform all these examinations, further to Dr B. Mravunac and Dr Z. Radanov for having carried out vaccination in children and for the clinical examination of post vaccination reactions.

New Thoughts on Pertussis

PEDIATRICS ◽  
1979 ◽  
Vol 63 (6) ◽  
pp. 941-942
Author(s):  
Samuel L. Katz ◽  
Catherine M. Wilfert
Keyword(s):  
United States ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Sharp Decrease ◽  
The United States ◽  
Critical Examination ◽  
Immunization Programs

The success of initial pertussis immunization programs resulted not only in a sharp decrease in the incidence of the disease but also in a diminution of interest in and research on the organism, and complacency with regard to the vaccine itself. Concern and apprehension about both the efficacy and the safety of pertussis vaccine have recently stimulated a renewed investigative interest in the biology of Bordetella pertussis. This occurs at a time. when cases of pertussis reported in the United States are at an all-time low (less than 2,000 in 1978 contrasted with more than 150,000 in 1950). This infrequency of clinical whooping cough contributes to our desire for a more critical examination of the rare serious complications attributed to the immunizing procedure.

scholarly journals CONSTRUCTION OF RECOMBINANT ATTENUATED BORDETELLA PERTUSSIS BACTERIA OF PTXP3 GENOTYPE

2018 ◽  
pp. 33-41 ◽  
Author(s):  
E. G. Semin ◽  
L. N. Sinyashina ◽  
A. Yu. Medkova ◽  
G. I. Karataev
Keyword(s):  
Bordetella Pertussis ◽  
Culture Medium ◽  
Genetically Engineered ◽  
Target Sequence ◽  
Vaccine Production ◽  
Dermonecrotic Toxin ◽  
Modified Dna ◽  
New Genotype ◽  
The Stability ◽  
Stability Of Structure

Aim. The construction of recombinant attenuated Bordetella pertussis bacteria of ptxP3 genotype and its genetic and biological stability characteristics. Materials and methods. During construction of recombinant attenuated bacteria of ptxP3 genotype virulent Bordetella pertussis bacteria of ptxP1 genotype were used as a recipient. PtxP1 genotype bacteria are used for whole cell pertussis (wP) vaccine production in Russia. Mutant bacteria B. pertussis 475 were received by crossing-over between chromosome comprising native copy of target sequence and its mutant copy in recombinant suicide plasmid transferred in recipient bacteria by conjugation. Genetically engineered construction of recombinant plasmids was conducted. The structure of modified chromosome locus of attenuated bacteria was determined by PCR and amplification fragments sequence. The stability of structure and characteristics of attenuated bacteria was defined after 15 passages of strains on culture medium and 5 passages in mice. Results. Isogenic attenuated ptxP1 B. pertussis 4M and ptxP3 B. pertussis 4MKS were constructed. These bacteria produce non-toxic pertussis toxin (PT) and do not produce dermonecrotic toxin (DNT). The promoter region of ptx operon contains mutation, typical for «new» genotype of circulating virulent bacteria and increasing PT production. The structure of modified DNA fragments and characteristics of attenuated bacteria did not change while storing and after passages on culture medium and in mice. Conclusion. Recombinant attenuated bacteria B. pertussis 4MKS of «new» ptxP3 genotype are constructed. Application perspectiveness of genetically engineered modification of isogenic B.pertussis bacteria for pertussis vaccines development is shown.

scholarly journals Complete Genome Sequences of Bordetella pertussis Vaccine Reference Strains 134 and 10536

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Michael R. Weigand ◽  
Yanhui Peng ◽  
Vladimir Loparev ◽  
Dhwani Batra ◽  
Mark Burroughs ◽  
...  
Keyword(s):  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Complete Genome ◽  
Whooping Cough ◽  
Genome Sequences ◽  
Vaccination Programs ◽  
Reference Strains

Vaccine formulations and vaccination programs against whooping cough (pertussis) vary worldwide. Here, we report the complete genome sequences of two divergent Bordetella pertussis reference strains used in the production of pertussis vaccines.

Whooping cough and pertussis vaccine

BMJ ◽  
1983 ◽  
Vol 287 (6398) ◽  
pp. 1068-1068
Author(s):  
A H Griffith
Keyword(s):  
Pertussis Vaccine ◽  
Whooping Cough

scholarly journals Bordetella pertussis Lipid A Glucosamine Modification Confers Resistance to Cationic Antimicrobial Peptides and Increases Resistance to Outer Membrane Perturbation

2014 ◽  
Vol 58 (8) ◽  
pp. 4931-4934 ◽  
Author(s):  
Nita R. Shah ◽  
Robert E. W. Hancock ◽  
Rachel C. Fernandez
Keyword(s):  
Immune System ◽  
Antimicrobial Peptides ◽  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Lipid A ◽  
Whooping Cough ◽  
Human Immune System ◽  
Cationic Antimicrobial Peptides ◽  
Content Type ◽  
Membrane Perturbation

ABSTRACTBordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic antimicrobial peptides (CAMPs). We show modification of the lipid A region of LPS with glucosamine increased resistance to numerous CAMPs, including LL-37. Furthermore, we demonstrate that this glucosamine modification increased resistance to outer membrane perturbation.

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