α-1,4-glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds

1993 ◽  
Vol 1156 (3) ◽  
pp. 313-320 ◽  
Author(s):  
Shukun Yu ◽  
Lennart Kenne ◽  
Marianne Pedersén
Keyword(s):  
Purification And Characterization ◽  
Degrading Enzyme ◽  
Red Seaweeds ◽  
New Class ◽  
Enzyme I

Purification and characterization of a glucoamylase secreted by the plant pathogenSclerotinia sclerotiorum

2002 ◽  
Vol 48 (3) ◽  
pp. 212-218 ◽  
Author(s):  
M B Martel ◽  
C Hervé du Penhoat ◽  
R Létoublon ◽  
M Fèvre
Keyword(s):  
Amino Acids ◽  
Sclerotinia Sclerotiorum ◽  
Reaction Products ◽  
Lytic Enzymes ◽  
Purification And Characterization ◽  
Degrading Enzyme

Among the lytic enzymes secreted by the phytopathogen fungus Sclerotinia sclerotiorum, a starch-degrading enzyme has been isolated and characterized. This glycoprotein of 72 kDa is composed of several isoforms ranging from pI 4.8 to 5.4. The enzymatic parameters have been determined. Specificity studies together with the analysis of the reaction products show that it is an α-1,4-glucanohydrolase. This result is also corroborated by the analysis of the N-terminal and two inner amino acids sequences that are very similar to fungal glucoamylase genes or enzymes so far sequenced.Key words: hydrolase, glucoamylase, Sclerotinia sclerotiorum, starch-degrading enzyme.

Purification and Characterization of a New Class of Insect α-Amylase Inhibitors from Barley

1997 ◽  
Vol 74 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Ningyan Zhang ◽  
Berne L. Jones ◽  
H. Peggy Tao
Keyword(s):  
Purification And Characterization ◽  
New Class ◽  
Amylase Inhibitors
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