The sensitivity of soybean tissue cultures to the thymidine analogue, 5-bromodeoxyuridine

1979 ◽  
Vol 17 (1) ◽  
pp. 123-128 ◽  
Author(s):  
T.L. Wang
1988 ◽  
Vol 7 (7) ◽  
pp. 477-480 ◽  
Author(s):  
Jonathan M. Greenberg ◽  
John F. Thompson ◽  
James T. Madison

1977 ◽  
Vol 60 (5) ◽  
pp. 754-758 ◽  
Author(s):  
Thomas S. Moore

Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).


Author(s):  
R. D. Sjolund ◽  
C. Y. Shih

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.


1993 ◽  
Vol 88 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Patrick von Aderkas ◽  
Parker Anderson

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